Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_D06_e332_08.seq
(1553 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 209 3e-54
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 209 4e-54
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 189 4e-48
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 109 5e-24
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 91 1e-18
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 79 6e-15
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 2.0
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 3.6
At5g08430.1 68418.m00993 SWIB complex BAF60b domain-containing p... 29 8.3
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 209 bits (511), Expect = 3e-54
Identities = 117/288 (40%), Positives = 153/288 (53%), Gaps = 2/288 (0%)
Frame = +3
Query: 135 NCEVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFY 308
+ EV FEEKF +D WE WV S+ + G++K TAG + DA DKG+QTSED RFY
Sbjct: 21 SAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDAN-DKGIQTSEDYRFY 78
Query: 309 ALSRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPD 488
A+S +F FSN+ K LV QFSVKHEQ +DCGGGY+K+ +DQ G+TPY IMFGPD
Sbjct: 79 AISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGPD 138
Query: 489 ICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEKV*IW* 668
ICG TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD TY +L DN +
Sbjct: 139 ICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILIDNVEKQTGS 198
Query: 669 IRSRLGLPTXEEDQGSXSKXNLKIWDHXXXXXXXXXXXXXXDWDKPLSXISDP*XP*ALX 848
+ S L ++ + +K + WD D P I D
Sbjct: 199 LYSDWDLLPAKKIKDPSAK-KPEDWDDKEYIPDPEDTKPAGYDDIP-KEIPDTDAK-KPE 255
Query: 849 IWMMRXXXXWNHPMXXTPDYXGXWAXXKLXPLLXRXXGXTPXXXXPEY 992
W W P P+Y G W K+ + P PE+
Sbjct: 256 DWDDEEDGEWTAPTIPNPEYNGEWKPKKIKNPAYKGKWKAPMIDNPEF 303
Score = 39.1 bits (87), Expect = 0.008
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +1
Query: 658 ESGELEADWDFLPXKKIKDPXAK 726
++G L +DWD LP KKIKDP AK
Sbjct: 195 QTGSLYSDWDLLPAKKIKDPSAK 217
Score = 35.9 bits (79), Expect = 0.072
Identities = 24/80 (30%), Positives = 29/80 (36%)
Frame = +2
Query: 731 PEDLGPQTHXSPDPEDNETRXLGQTLEXHFGPLMXVSPEXLDDEXXXXXEPPYDXXPXLX 910
PED + + PDPED + + PE DDE P P
Sbjct: 219 PEDWDDKEYI-PDPEDTKPAGYDD-IPKEIPDTDAKKPEDWDDEEDGEWTAPTIPNPEYN 276
Query: 911 GRLGPXQIXTPAXKGPWXHP 970
G P +I PA KG W P
Sbjct: 277 GEWKPKKIKNPAYKGKWKAP 296
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 209 bits (510), Expect = 4e-54
Identities = 99/170 (58%), Positives = 121/170 (71%), Gaps = 2/170 (1%)
Frame = +3
Query: 144 VFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYALS 317
V FEE+F DD WE+ WV SE + G++K TAG + DA DKG+QTSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDAN-DKGIQTSEDYRFYAIS 81
Query: 318 RKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPDICG 497
+F FSN+ K LV QFSVKHEQ +DCGGGY+K+ +DQK G+TPY IMFGPDICG
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICG 141
Query: 498 PGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDN 647
TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD TY +L DN
Sbjct: 142 YSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILIDN 191
Score = 38.3 bits (85), Expect = 0.014
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +1
Query: 658 ESGELEADWDFLPXKKIKDPXAK 726
++G L +DWD LP KKIKDP AK
Sbjct: 195 QTGSLYSDWDLLPPKKIKDPSAK 217
Score = 35.1 bits (77), Expect = 0.13
Identities = 25/80 (31%), Positives = 28/80 (35%)
Frame = +2
Query: 731 PEDLGPQTHXSPDPEDNETRXLGQTLEXHFGPLMXVSPEXLDDEXXXXXEPPYDXXPXLX 910
PED Q + S DPED + + PE DDE P P
Sbjct: 219 PEDWDEQEYIS-DPEDKKPDGYDD-IPKEIPDTDSKKPEDWDDEEDGEWTAPTIPNPEYM 276
Query: 911 GRLGPXQIXTPAXKGPWXHP 970
G P QI P KG W P
Sbjct: 277 GEWKPKQIKNPNYKGKWEAP 296
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 189 bits (461), Expect = 4e-48
Identities = 89/173 (51%), Positives = 121/173 (69%), Gaps = 2/173 (1%)
Frame = +3
Query: 141 EVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 314
E+F EE F + W+S WV S+ E G FK TAGK+ D + +KG+QT DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPD-NKGIQTYNDAKHYAI 86
Query: 315 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPDIC 494
S K FSN+ + LVVQ+SVK EQDI+CGG Y+K+ ++QK G+TPY +MFGPDIC
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDIC 146
Query: 495 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEK 653
G TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD +Y VL DN++
Sbjct: 147 GTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKE 199
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 109 bits (262), Expect = 5e-24
Identities = 54/114 (47%), Positives = 75/114 (65%), Gaps = 2/114 (1%)
Frame = +3
Query: 141 EVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 314
E+F EE F + W+S WV S+ E G FK TAGK+ D + +KG+QT DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPD-NKGIQTYNDAKHYAI 86
Query: 315 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIM 476
S K FSN+ + LVVQ+SVK EQDI+CGG Y+K+ ++QK G+TPY ++
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 91.5 bits (217), Expect = 1e-18
Identities = 56/172 (32%), Positives = 89/172 (51%), Gaps = 10/172 (5%)
Frame = +3
Query: 168 DDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYALSRKF-KPFSNE 344
D+ ++ W+ S++ E G +K ++ D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 345 GKPLVVQFSVKHEQDIDCGGGYLKVF---DCRLDQKDMHGETPYEIMFGPDICGPGTKKV 515
+V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG GT KV
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG-GTNKV 149
Query: 516 HVIFSYKGK------NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEK 653
H I +K H +K D +H+YT I+KPDN +L D E+
Sbjct: 150 HFILKHKNPKSGEYVEHHLKFPPSVPYDKLSHVYTAILKPDNEVRILVDGEE 201
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 79.4 bits (187), Expect = 6e-15
Identities = 54/175 (30%), Positives = 89/175 (50%), Gaps = 13/175 (7%)
Frame = +3
Query: 168 DDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYALSRKF---KPFS 338
D+ ++ WV SE + G +K + ++D GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEKSEGHDDY----GLLVSEKAKKYGIVKELDVDEPLN 90
Query: 339 -NEGKPLVVQFSVKHEQDIDCGGGYLKVF---DCRLDQKDMHGETPYEIMFGPDICGPGT 506
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-AT 148
Query: 507 KKVHVIFSYKGK------NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEK 653
KVH I +K H +K D+ +H+YT ++K DN +L D E+
Sbjct: 149 NKVHFILKHKNPKSGEFVEHHLKFPPSVPFDMLSHVYTAVLKSDNEVRILVDGEE 203
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 2.0
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 360 VQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEI 473
++F+ KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 3.6
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +3
Query: 360 VQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYE 470
++F+ KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At5g08430.1 68418.m00993 SWIB complex BAF60b domain-containing
protein / plus-3 domain-containing protein / GYF
domain-containing protein similar to CPRF interacting
protein [Petroselinum crispum] GI:9588690; contains Pfam
profiles PF02201: BAF60b domain of the SWIB complex,
PF03126: Plus-3 domain, PF02213: GYF domain; contains
non-conensus AT-AC splice sites at intron 5
Length = 553
Score = 29.1 bits (62), Expect = 8.3
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +3
Query: 165 SDDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAE 266
S D + NW+Y + G G F LT K ++DAE
Sbjct: 492 SYDKEKLNWLYKDPQGLVQGPFSLTQLKAWSDAE 525
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,755,130
Number of Sequences: 28952
Number of extensions: 418396
Number of successful extensions: 914
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 906
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4173510336
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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