Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_D04_e316_08.seq
         (1542 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC4G3.04c |coq5||C-methytransferase |Schizosaccharomyces pombe...   149   8e-37
SPAC56F8.09 |rrp8||rRNA methyltransferase Rrp8 |Schizosaccharomy...    31   0.56 
SPAC25B8.09 |||trans-aconitate 3-methyltransferase |Schizosaccha...    28   3.0  
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom...    27   6.9  
SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch...    27   9.1  

>SPCC4G3.04c |coq5||C-methytransferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 305

 Score =  149 bits (362), Expect = 8e-37
 Identities = 70/126 (55%), Positives = 91/126 (72%), Gaps = 3/126 (2%)
 Frame = +1

Query: 109 LPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEFSHLPNNTLQWMYDQYSF 288
           +PD S   YTIAFGIRNCTHI KVLE+AYRVL PGG F CLEFS +    L  +Y QYSF
Sbjct: 180 IPDNSIDMYTIAFGIRNCTHIPKVLEQAYRVLKPGGVFSCLEFSKVYPAPLAELYRQYSF 239

Query: 289 QVIPVMGQLVAGQWKPYQYLVESIRQFPNQEKFKGMIEEAGFR---QVTYENLTFGTVAI 459
           +++P++G ++AG  + Y+YLVESI +FP+ + F  MIE+AGF    +  YE L+FG  AI
Sbjct: 240 KILPLLGTIIAGDSQSYEYLVESIERFPDAKTFAKMIEDAGFTLAGETGYETLSFGIAAI 299

Query: 460 HSGFKI 477
           H+G K+
Sbjct: 300 HTGIKL 305


>SPAC56F8.09 |rrp8||rRNA methyltransferase Rrp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 318

 Score = 30.7 bits (66), Expect = 0.56
 Identities = 15/45 (33%), Positives = 23/45 (51%)
 Frame = +1

Query: 82  VCADAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGG 216
           V  D  N+P+ DE+         +   T+ +  L+EAYR+L  GG
Sbjct: 206 VACDIANVPMADETVDIAVFCLSLMG-TNWQSFLKEAYRILKVGG 249


>SPAC25B8.09 |||trans-aconitate 3-methyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 251

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 20/59 (33%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
 Frame = +1

Query: 103 LPLPDESYSAYTIAFGIRNCTHI---EKVLEEAYRVLVPGGRFMCLEFSHLPNNTLQWM 270
           +PL DES     +A G   C H    E+ L+E YRVL P G+   +   ++ +N++ W+
Sbjct: 92  IPLEDESVDL--VACG--QCFHWFANEEALKEIYRVLKPNGKLALI--WNIRDNSVPWV 144


>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 27.1 bits (57), Expect = 6.9
 Identities = 13/47 (27%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
 Frame = +1

Query: 115 DESYSAYTIAFGIRN--CTHIEKVLEEAYRVLVPGGRFMCLEFSHLP 249
           +E  + +++ F  RN   +++   L+     L+ G RF+ L F H P
Sbjct: 435 EEFRNGHSVVFADRNNHISNMRSTLQTDILALIDGVRFVALPFKHTP 481


>SPBC16E9.05 |erg6||delta-sterol C-methyltransferase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 26.6 bits (56), Expect = 9.1
 Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
 Frame = +1

Query: 79  WVCADAENLPLPDESYS-AYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEF 237
           +V  D  ++P  D ++   Y I   + +   +E V  E +RVL PGG F   E+
Sbjct: 178 FVKGDFMHMPFEDNTFDYVYAIEATV-HAPSLEGVYGEIFRVLKPGGVFGVYEW 230


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,798,000
Number of Sequences: 5004
Number of extensions: 67738
Number of successful extensions: 142
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 866210038
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)