Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_D04_e316_08.seq
(1542 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57300.1 68418.m07158 UbiE/COQ5 methyltransferase family prot... 170 2e-42
At1g23360.3 68414.m02922 UbiE/COQ5 methyltransferase family prot... 62 9e-10
At1g23360.2 68414.m02921 UbiE/COQ5 methyltransferase family prot... 62 9e-10
At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family prot... 62 9e-10
At3g63410.1 68416.m07139 chloroplast inner envelope membrane pro... 33 0.38
At1g69523.1 68414.m07991 UbiE/COQ5 methyltransferase family prot... 33 0.66
At1g20330.1 68414.m02537 S-adenosyl-methionine-sterol-C-methyltr... 32 0.88
At3g01660.1 68416.m00097 expressed protein similar to putative p... 31 1.5
At2g31740.1 68415.m03876 expressed protein 31 2.0
At1g69520.1 68414.m07990 methyltransferase-related weak similari... 30 4.7
At5g57230.1 68418.m07150 expressed protein 29 6.2
At4g29590.1 68417.m04218 expressed protein 29 8.2
>At5g57300.1 68418.m07158 UbiE/COQ5 methyltransferase family protein
similar to ubiquinone biosynthesis methyltransferase
COQ5 [Saccharomyces cerevisiae][SP|P49017],
ubiquinone/menaquinone biosynthesis methyltransferase
ubiE [Escherichia coli][SP|P27851]; contains Pfam
profile PF01209: methlytransferase, UbiE/COQ5 family
Length = 288
Score = 170 bits (413), Expect = 2e-42
Identities = 81/133 (60%), Positives = 95/133 (71%)
Frame = +1
Query: 79 WVCADAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEFSHLPNNT 258
WV DAE L D S YTIAFGIRN THIEK L EAYRVL GGRF+CLE SH+
Sbjct: 156 WVEGDAEALSFDDNSMDGYTIAFGIRNVTHIEKALAEAYRVLKRGGRFLCLELSHVEIPV 215
Query: 259 LQWMYDQYSFQVIPVMGQLVAGQWKPYQYLVESIRQFPNQEKFKGMIEEAGFRQVTYENL 438
+ +YD YSFQVIP +G+L+AG + YQYLVES+R+FP QE+F MI +AGF +V YENL
Sbjct: 216 FKNLYDLYSFQVIPNLGELIAGDRESYQYLVESVRRFPPQERFASMIADAGFEKVEYENL 275
Query: 439 TFGTVAIHSGFKI 477
G VAIHS K+
Sbjct: 276 VGGVVAIHSAIKL 288
>At1g23360.3 68414.m02922 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 160
Score = 62.1 bits (144), Expect = 9e-10
Identities = 36/123 (29%), Positives = 59/123 (47%)
Frame = +1
Query: 79 WVCADAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEFSHLPNNT 258
W+ DA +LP D + A T+ +G+RN + ++E YRVL PG R L+F+ N +
Sbjct: 30 WIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRLRAMKEMYRVLKPGSRVSILDFNK-SNQS 88
Query: 259 LQWMYDQYSFQVIPVMGQLVAGQWKPYQYLVESIRQFPNQEKFKGMIEEAGFRQVTYENL 438
+ + + V V K Y+YL SI + E+ + + EAGF + +
Sbjct: 89 VTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETLALEAGFSSACHYEI 148
Query: 439 TFG 447
+ G
Sbjct: 149 SGG 151
>At1g23360.2 68414.m02921 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 160
Score = 62.1 bits (144), Expect = 9e-10
Identities = 36/123 (29%), Positives = 59/123 (47%)
Frame = +1
Query: 79 WVCADAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEFSHLPNNT 258
W+ DA +LP D + A T+ +G+RN + ++E YRVL PG R L+F+ N +
Sbjct: 30 WIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRLRAMKEMYRVLKPGSRVSILDFNK-SNQS 88
Query: 259 LQWMYDQYSFQVIPVMGQLVAGQWKPYQYLVESIRQFPNQEKFKGMIEEAGFRQVTYENL 438
+ + + V V K Y+YL SI + E+ + + EAGF + +
Sbjct: 89 VTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETLALEAGFSSACHYEI 148
Query: 439 TFG 447
+ G
Sbjct: 149 SGG 151
>At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 198
Score = 62.1 bits (144), Expect = 9e-10
Identities = 36/123 (29%), Positives = 59/123 (47%)
Frame = +1
Query: 79 WVCADAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEFSHLPNNT 258
W+ DA +LP D + A T+ +G+RN + ++E YRVL PG R L+F+ N +
Sbjct: 68 WIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRLRAMKEMYRVLKPGSRVSILDFNK-SNQS 126
Query: 259 LQWMYDQYSFQVIPVMGQLVAGQWKPYQYLVESIRQFPNQEKFKGMIEEAGFRQVTYENL 438
+ + + V V K Y+YL SI + E+ + + EAGF + +
Sbjct: 127 VTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETLALEAGFSSACHYEI 186
Query: 439 TFG 447
+ G
Sbjct: 187 SGG 189
>At3g63410.1 68416.m07139 chloroplast inner envelope membrane
protein, putative (APG1) similar to SP|P23525 37 kDa
inner envelope membrane protein, chloroplast precursor
(E37) {Spinacia oleracea}; contains Pfam profile
PF01209: methlytransferase, UbiE/COQ5 family
Length = 338
Score = 33.5 bits (73), Expect = 0.38
Identities = 19/47 (40%), Positives = 25/47 (53%)
Frame = +1
Query: 91 DAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCL 231
DAE+LP P + Y A I ++ + EAYRVL GG+ CL
Sbjct: 165 DAEDLPFPTDYADRYVSAGSIEYWPDPQRGIREAYRVLKIGGK-ACL 210
>At1g69523.1 68414.m07991 UbiE/COQ5 methyltransferase family protein
low similarity to SP|Q05197 Phosphatidylethanolamine
N-methyltransferase (EC 2.1.1.17) [Rhodopseudomonas
sphaeroides] {Rhodobacter sphaeroides}; contains Pfam
profile PF01209: methlytransferase, UbiE/COQ5 family
Length = 300
Score = 32.7 bits (71), Expect = 0.66
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +1
Query: 79 WVCADAENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLE 234
+V A E++PL D S A + + T + + L+E R+L PGG ++ +E
Sbjct: 179 FVHALGESIPLEDASVDAVVGTLVLCSVTDVTQTLKEIKRILRPGGIYIFIE 230
>At1g20330.1 68414.m02537
S-adenosyl-methionine-sterol-C-methyltransferase
identical to sterol-C-methyltransferase GI:1061040 from
[Arabidopsis thaliana]
Length = 361
Score = 32.3 bits (70), Expect = 0.88
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +1
Query: 82 VCADAENLPLPDESYS-AYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEF 237
VC + +P D S+ AY+I + +E+V E YRVL PG ++ E+
Sbjct: 178 VCGNFLQMPFDDNSFDGAYSIE-ATCHAPKLEEVYAEIYRVLKPGSMYVSYEW 229
>At3g01660.1 68416.m00097 expressed protein similar to putative
protein GB:CAB45319 [Arabidopsis thaliana]
Length = 273
Score = 31.5 bits (68), Expect = 1.5
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +1
Query: 115 DESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLEFSH 243
D+ + A + G++ EKV E YRVL PGG + + FS+
Sbjct: 152 DKYFDAVLCSVGVQYLQQPEKVFAEVYRVLKPGG-VLIVSFSN 193
>At2g31740.1 68415.m03876 expressed protein
Length = 760
Score = 31.1 bits (67), Expect = 2.0
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +1
Query: 181 LEEAYRVLVPGGRFMCLEFSHLPNNTLQWMYDQYSF 288
L EA RVL PGG+F+CL + ++ L ++ ++ F
Sbjct: 161 LSEAKRVLKPGGKFICLTLAE--SHVLALLFSRFRF 194
>At1g69520.1 68414.m07990 methyltransferase-related weak similarity
to SP|Q05197 Phosphatidylethanolamine
N-methyltransferase (EC 2.1.1.17) {Rhodobacter
sphaeroides}; contains domain similarity with PF01209:
methyltransferase, UbiE/COQ5 family
Length = 262
Score = 29.9 bits (64), Expect = 4.7
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +1
Query: 97 ENLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGGRFMCLE 234
E +PL D S A + + + + + L+E RVL GG F+ LE
Sbjct: 146 EAIPLKDNSVDAVVATLVLCSVSDVTQTLKEIKRVLRQGGVFIFLE 191
>At5g57230.1 68418.m07150 expressed protein
Length = 160
Score = 29.5 bits (63), Expect = 6.2
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
Frame = +1
Query: 88 ADAENLPLPDESYSAYTIAFGIR-NCT-HIEKVLEEAYRVLVPGGRFMCLEFSHLPNNTL 261
++ E + L + Y +AF IR N T H++++LEEA P +FM +E P +
Sbjct: 38 SEREFVQLLHQGYPV-VVAFTIRSNYTQHLDRMLEEAAAEFYPNIKFMRVECPKYPGFCI 96
Query: 262 QWMYDQYSF 288
++Y F
Sbjct: 97 TRQKNEYPF 105
>At4g29590.1 68417.m04218 expressed protein
Length = 317
Score = 29.1 bits (62), Expect = 8.2
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +1
Query: 100 NLPLPDESYSAYTIAFGIRNCTHIEKVLEEAYRVLVPGG 216
NLP D S+ T + T +V +E R+L PGG
Sbjct: 209 NLPFEDNSFQVITNVVSVDYLTKPLEVFKEMNRILKPGG 247
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,649,102
Number of Sequences: 28952
Number of extensions: 374171
Number of successful extensions: 754
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 751
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4134955968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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