Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_D02_e300_08.seq
         (1493 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) ...    30   3.4  
At2g26030.2 68415.m03125 F-box family protein contains F-box dom...    30   4.5  
At2g26030.1 68415.m03126 F-box family protein contains F-box dom...    30   4.5  

>At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) /
           ferredoxin-dependent glutamate synthase (Fd-GOGAT 2)
           identical to SP|Q9T0P4 Ferredoxin-dependent glutamate
           synthase 2, chloroplast precursor (EC 1.4.7.1) (Fd-GOGAT
           2) {Arabidopsis thaliana}
          Length = 1629

 Score = 30.3 bits (65), Expect = 3.4
 Identities = 29/109 (26%), Positives = 48/109 (44%), Gaps = 1/109 (0%)
 Frame = +2

Query: 350 LFIDFKAI-KSKAKALIGNEWVFRATASVA*LYYCMTRSEVSGSICYPTINFFLLSIPES 526
           L+I  K I ++ A     +E  F + ++   +Y  M RSEV G + YP +   L   P  
Sbjct: 255 LYICRKLIERAVASESWASELYFSSLSNQTIVYKGMLRSEVLG-LFYPDLQNDLYKSP-- 311

Query: 527 GQVIWDFSTQYHPGVWNLCPIWRQARPLSHPGRN*ALRNVGGVVHYFTS 673
                 F+  +     N  P W  A+P+   G N  +  + G +++ TS
Sbjct: 312 ------FAIYHRRFSTNTSPRWHLAQPMRFLGHNGEINTIQGNLNWMTS 354


>At2g26030.2 68415.m03125 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 369

 Score = 29.9 bits (64), Expect = 4.5
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = +3

Query: 639 EMWVV*FTISPLPNPSGITGVSLCLFQS*NVVPVREVV 752
           E  ++ FT+S  P   G+T V  CL  S   V +RE++
Sbjct: 273 ENLILDFTVSTEPEQDGLTYVPQCLLSSLECVEIRELI 310


>At2g26030.1 68415.m03126 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 442

 Score = 29.9 bits (64), Expect = 4.5
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = +3

Query: 639 EMWVV*FTISPLPNPSGITGVSLCLFQS*NVVPVREVV 752
           E  ++ FT+S  P   G+T V  CL  S   V +RE++
Sbjct: 346 ENLILDFTVSTEPEQDGLTYVPQCLLSSLECVEIRELI 383


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,337,539
Number of Sequences: 28952
Number of extensions: 448449
Number of successful extensions: 897
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 867
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 897
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3980738496
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)