BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_D01_e292_07.seq
(1545 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 31 0.32
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 9.2
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 9.2
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 31.5 bits (68), Expect = 0.32
Identities = 14/64 (21%), Positives = 34/64 (53%)
Frame = +2
Query: 359 FQLLVGSMTFFYMINYGKMKHHRNYKYH*RSNSTINLFVHENIIIQDSTGLSHVYISYCN 538
F+ + G Y+I++GK+K + K+ + S ++ ++H+N ++ + ++ IS
Sbjct: 220 FEFVDGGQMLDYIISHGKLKEKQARKFVRQIGSALS-YLHQNSVVHRDLKIENILISKTG 278
Query: 539 KISV 550
I +
Sbjct: 279 DIKI 282
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 26.6 bits (56), Expect = 9.2
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
Frame = +2
Query: 593 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 757
P P+ S ++ H + + +L N I+L ++PLSP A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231
Query: 758 SCA 766
S A
Sbjct: 232 SDA 234
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 26.6 bits (56), Expect = 9.2
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 700 PPFASWRNSEEARTDRPSQQLRSLN 774
PP AS RN E + PS+Q S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,343,096
Number of Sequences: 5004
Number of extensions: 86757
Number of successful extensions: 160
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 868192212
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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