Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_C05_e323_05.seq
(1523 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 68 6e-10
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 68 6e-10
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 68 6e-10
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 68 6e-10
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 66 2e-09
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:... 51 9e-05
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba... 49 3e-04
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ... 47 0.002
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp... 44 0.011
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ... 37 1.2
UniRef50_Q0D4Y8 Cluster: Os07g0596300 protein; n=7; Eukaryota|Re... 36 2.1
UniRef50_A5FB10 Cluster: Fatty acid desaturase; n=1; Flavobacter... 36 3.7
UniRef50_A4C0I3 Cluster: DNA binding protein; n=4; Flavobacteria... 36 3.7
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la... 36 3.7
UniRef50_Q60R78 Cluster: Putative uncharacterized protein CBG214... 35 4.9
UniRef50_A7SCY0 Cluster: Predicted protein; n=2; Nematostella ve... 34 8.6
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 68.1 bits (159), Expect = 6e-10
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = +2
Query: 755 LAVVLHRRDWENPGVTHLNRLAAHPPFASWR 847
LAVVL RRDWENPGVT LNRLAAHPPFASWR
Sbjct: 68 LAVVLQRRDWENPGVTQLNRLAAHPPFASWR 98
Score = 44.8 bits (101), Expect = 0.006
Identities = 20/28 (71%), Positives = 21/28 (75%)
Frame = +1
Query: 829 PFRQLAYSEEARTDRPSQQLRSLXGEWQ 912
PF SEEARTDRPSQQLR L GEW+
Sbjct: 93 PFASWRNSEEARTDRPSQQLRXLNGEWR 120
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 68.1 bits (159), Expect = 6e-10
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = +2
Query: 755 LAVVLHRRDWENPGVTHLNRLAAHPPFASWR 847
LAVVL RRDWENPGVT LNRLAAHPPFASWR
Sbjct: 22 LAVVLQRRDWENPGVTQLNRLAAHPPFASWR 52
Score = 37.9 bits (84), Expect = 0.69
Identities = 18/23 (78%), Positives = 18/23 (78%)
Frame = +1
Query: 829 PFRQLAYSEEARTDRPSQQLRSL 897
PF SEEARTDRPSQQLRSL
Sbjct: 47 PFASWRNSEEARTDRPSQQLRSL 69
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 68.1 bits (159), Expect = 6e-10
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = +2
Query: 755 LAVVLHRRDWENPGVTHLNRLAAHPPFASWR 847
LAVVL RRDWENPGVT LNRLAAHPPFASWR
Sbjct: 26 LAVVLQRRDWENPGVTQLNRLAAHPPFASWR 56
Score = 46.8 bits (106), Expect = 0.002
Identities = 21/28 (75%), Positives = 22/28 (78%)
Frame = +1
Query: 829 PFRQLAYSEEARTDRPSQQLRSLXGEWQ 912
PF SEEARTDRPSQQLRSL GEW+
Sbjct: 51 PFASWRNSEEARTDRPSQQLRSLNGEWR 78
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 68.1 bits (159), Expect = 6e-10
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = +2
Query: 755 LAVVLHRRDWENPGVTHLNRLAAHPPFASWR 847
LAVVL RRDWENPGVT LNRLAAHPPFASWR
Sbjct: 8 LAVVLQRRDWENPGVTQLNRLAAHPPFASWR 38
Score = 46.8 bits (106), Expect = 0.002
Identities = 21/28 (75%), Positives = 22/28 (78%)
Frame = +1
Query: 829 PFRQLAYSEEARTDRPSQQLRSLXGEWQ 912
PF SEEARTDRPSQQLRSL GEW+
Sbjct: 33 PFASWRNSEEARTDRPSQQLRSLNGEWR 60
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba
histolytica HM-1:IMSS
Length = 86
Score = 66.5 bits (155), Expect = 2e-09
Identities = 29/32 (90%), Positives = 29/32 (90%)
Frame = +3
Query: 753 HWPSFYTVXTGKTLALPTLIALQHIPLSPAGV 848
HWPSFY V TGKTLALP LIALQHIPLSPAGV
Sbjct: 5 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGV 36
Score = 35.1 bits (77), Expect = 4.9
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +1
Query: 850 SEEARTDRPSQQLRSL 897
SEEARTDRPSQQLRSL
Sbjct: 38 SEEARTDRPSQQLRSL 53
>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
Beta-galactosidase - Yersinia pseudotuberculosis
Length = 1066
Score = 50.8 bits (116), Expect = 9e-05
Identities = 20/31 (64%), Positives = 23/31 (74%)
Frame = +2
Query: 755 LAVVLHRRDWENPGVTHLNRLAAHPPFASWR 847
L +L RRDWENP +T +RL AHPPF SWR
Sbjct: 15 LPQILSRRDWENPQITQYHRLEAHPPFHSWR 45
>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
pneumoniae
Length = 1034
Score = 49.2 bits (112), Expect = 3e-04
Identities = 20/28 (71%), Positives = 21/28 (75%)
Frame = +2
Query: 764 VLHRRDWENPGVTHLNRLAAHPPFASWR 847
VL R DW N +THLNRL AHP FASWR
Sbjct: 17 VLAREDWHNQTITHLNRLPAHPVFASWR 44
>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 1043
Score = 46.8 bits (106), Expect = 0.002
Identities = 18/35 (51%), Positives = 23/35 (65%)
Frame = +2
Query: 755 LAVVLHRRDWENPGVTHLNRLAAHPPFASWRIAKR 859
LA +L R DW+NP +T +NRL +H P WR A R
Sbjct: 18 LATILARNDWQNPAITSVNRLPSHTPLHGWRDADR 52
>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
spumigena CCY 9414
Length = 72
Score = 44.0 bits (99), Expect = 0.011
Identities = 19/21 (90%), Positives = 20/21 (95%)
Frame = +1
Query: 850 SEEARTDRPSQQLRSLXGEWQ 912
SEEARTDRPSQQLRSL GEW+
Sbjct: 50 SEEARTDRPSQQLRSLNGEWR 70
>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein - Plasmodium berghei
Length = 275
Score = 37.1 bits (82), Expect = 1.2
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +3
Query: 702 RGGARYPIRPIVSRIT 749
RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275
>UniRef50_Q0D4Y8 Cluster: Os07g0596300 protein; n=7; Eukaryota|Rep:
Os07g0596300 protein - Oryza sativa subsp. japonica
(Rice)
Length = 754
Score = 36.3 bits (80), Expect = 2.1
Identities = 37/128 (28%), Positives = 39/128 (30%)
Frame = +1
Query: 1039 SPXKTGPPQGXNRXXRGPSDPPXSFGAGXXXNGPKXPXXPXGNPNXQKXXNXGGLA*XXX 1218
SP PP G GP PP GA P P P G P+ GG A
Sbjct: 178 SPPPPPPPPGARP---GPPPPPPPPGA--RPGPPPPPPPPGGRPSAPPLPPPGGRA--SA 230
Query: 1219 XPXPPP*AXGXGLXKAXXVFNPXPRXPSX*XXGPXXXLGXXXPPXKXXGXXPXXXXPXXX 1398
P PPP + G R P P LG PP G P P
Sbjct: 231 PPPPPPPSTRLGAPPPPPPPGAGGRAPPP-PPAPGGRLGGPPPPPPPGGRAP----PPPR 285
Query: 1399 XXXXPXPP 1422
P PP
Sbjct: 286 GPGAPPPP 293
>UniRef50_A5FB10 Cluster: Fatty acid desaturase; n=1; Flavobacterium
johnsoniae UW101|Rep: Fatty acid desaturase -
Flavobacterium johnsoniae UW101
Length = 386
Score = 35.5 bits (78), Expect = 3.7
Identities = 20/49 (40%), Positives = 29/49 (59%), Gaps = 3/49 (6%)
Frame = +2
Query: 185 YYINMLSARPHLYFFCFGKSRILLFKSHVTLLFIFF---GFTSKVIFIN 322
Y+ N++ + L F S IL F S+ +LLF+F+ GFT V+FIN
Sbjct: 55 YHFNVIKSLGLLVLFFLFYSCILFFGSNTSLLFLFYILCGFTMIVLFIN 103
>UniRef50_A4C0I3 Cluster: DNA binding protein; n=4;
Flavobacteriaceae|Rep: DNA binding protein -
Polaribacter irgensii 23-P
Length = 377
Score = 35.5 bits (78), Expect = 3.7
Identities = 16/46 (34%), Positives = 30/46 (65%)
Frame = +2
Query: 107 MLADIYIQVVIQPASILNSLNYCSFDYYINMLSARPHLYFFCFGKS 244
M+A I++Q+++ +IL SL+ +FD+Y+ + + L+FF F S
Sbjct: 183 MIAIIFLQIIVFITTILGSLDSGAFDFYLKIETLVYTLFFFIFAFS 228
>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
lactis|Rep: Beta-galactosidase - Lactococcus lactis
subsp. lactis (Streptococcus lactis)
Length = 998
Score = 35.5 bits (78), Expect = 3.7
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +2
Query: 764 VLHRRDWENPGVTHLNRLAAHPP 832
VL R+DWENP V++ NRL H P
Sbjct: 9 VLERKDWENPVVSNWNRLPMHTP 31
>UniRef50_Q60R78 Cluster: Putative uncharacterized protein CBG21491;
n=1; Caenorhabditis briggsae|Rep: Putative
uncharacterized protein CBG21491 - Caenorhabditis
briggsae
Length = 429
Score = 35.1 bits (77), Expect = 4.9
Identities = 31/130 (23%), Positives = 38/130 (29%)
Frame = +1
Query: 1033 GQSPXKTGPPQGXNRXXRGPSDPPXSFGAGXXXNGPKXPXXPXGNPNXQKXXNXGGLA*X 1212
G+ P PP G R + + GA + P P G+P G
Sbjct: 270 GRGPRTGSPPTGSPPTGRPMREKRQAPGAPPTGSPPPRPSGAPGSPPPPPP---SGAPPT 326
Query: 1213 XXXPXPPP*AXGXGLXKAXXVFNPXPRXPSX*XXGPXXXLGXXXPPXKXXGXXPXXXXPX 1392
P PPP + G A +P PR G G PP G P
Sbjct: 327 GSPPPPPPQSGGSPPPGAPPSGSPPPRPSGAPPAGGSPPTGSPPPPSPQGGAGPRGKRQV 386
Query: 1393 XXXXXXPXPP 1422
P PP
Sbjct: 387 SGVQPPPPPP 396
>UniRef50_A7SCY0 Cluster: Predicted protein; n=2; Nematostella
vectensis|Rep: Predicted protein - Nematostella vectensis
Length = 906
Score = 34.3 bits (75), Expect = 8.6
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +2
Query: 1226 PPPPRPXGXGXGRPPXFLTQ 1285
PPPPRP G G+PP F++Q
Sbjct: 846 PPPPRPFPMGAGQPPSFMSQ 865
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,088,760,268
Number of Sequences: 1657284
Number of extensions: 19985865
Number of successful extensions: 44995
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 40672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 44603
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 162521628425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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