Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_C04_e315_06.seq
         (1570 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi...   135   9e-32
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)           133   3e-31
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)           128   1e-29
At5g63900.1 68418.m08023 PHD finger family protein contains Pfam...    30   3.6  
At1g32140.1 68414.m03954 F-box family protein contains F-box dom...    29   6.3  
At4g34630.1 68417.m04918 expressed protein                             29   8.4  
At1g66070.1 68414.m07499 translation initiation factor-related s...    29   8.4  

>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
           to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
           tuberosum]
          Length = 135

 Score =  135 bits (326), Expect = 9e-32
 Identities = 58/120 (48%), Positives = 85/120 (70%), Gaps = 1/120 (0%)
 Frame = +1

Query: 145 GRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSKIKPFVKGV 324
           GRYAG+KA+++K++D+GTSD+ YGH  VAG+ +YP KV ++    K  K+S++K F+K V
Sbjct: 16  GRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLV 75

Query: 325 NYNHLMPTRYSVDXSFEKFSAKD-LKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 501
           NY HLMPTRY++D   ++ +  D LK   K+       + + EER+K+GKN+WFF KLRF
Sbjct: 76  NYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFKTGKNRWFFTKLRF 135


>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
          Length = 135

 Score =  133 bits (321), Expect = 3e-31
 Identities = 56/120 (46%), Positives = 84/120 (70%), Gaps = 1/120 (0%)
 Frame = +1

Query: 145 GRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSKIKPFVKGV 324
           GRYAG+KA+++K++D+G  D+PYGH  VAG+ +YP KV ++    K  K+S++K F+K V
Sbjct: 16  GRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLV 75

Query: 325 NYNHLMPTRYSVDXSFEKFSAKD-LKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 501
           NY HLMPTRY++D   ++ +  D L+   K+       + + EER+K+GKN+WFF KLRF
Sbjct: 76  NYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135


>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 
          Length = 135

 Score =  128 bits (308), Expect = 1e-29
 Identities = 55/120 (45%), Positives = 81/120 (67%), Gaps = 1/120 (0%)
 Frame = +1

Query: 145 GRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSKIKPFVKGV 324
           GRY G+KA++VK++D+GT +K YGH  VAG+ +YP KV ++    K  K+S++K F K +
Sbjct: 16  GRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFFKVI 75

Query: 325 NYNHLMPTRYSVDXSFEK-FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 501
           NY H+MPTRY++D   +   SA  +    K+       + +FEER+K+GKN+WFF KLRF
Sbjct: 76  NYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNRWFFTKLRF 135


>At5g63900.1 68418.m08023 PHD finger family protein contains Pfam
           domain, PF00628: PHD-finger
          Length = 557

 Score = 30.3 bits (65), Expect = 3.6
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = +1

Query: 427 FNTRVRFEERYKSGKNKWFF 486
           +N R + E+RYKS K KWF+
Sbjct: 58  YNKRNKKEQRYKSPKGKWFY 77


>At1g32140.1 68414.m03954 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 591

 Score = 29.5 bits (63), Expect = 6.3
 Identities = 14/35 (40%), Positives = 21/35 (60%)
 Frame = -2

Query: 300 FRPLVNFIFSHPFMNLARVSVDAGDESMSVWFVRC 196
           F PL++  FSH F+   R+S   G E ++V + RC
Sbjct: 227 FGPLLSLSFSHDFIETGRLSCVKG-EKLAVLYQRC 260


>At4g34630.1 68417.m04918 expressed protein
          Length = 199

 Score = 29.1 bits (62), Expect = 8.4
 Identities = 11/38 (28%), Positives = 21/38 (55%)
 Frame = +1

Query: 187 DEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 300
           D+G  D+ Y  ++    +  PR V K++G+ ++ K  K
Sbjct: 130 DDGEEDREYDDSYDLDEELVPRSVSKKVGRQRMRKLGK 167


>At1g66070.1 68414.m07499 translation initiation factor-related
           similar to Eukaryotic translation initiation factor 3
           subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
           (Swiss-Prot:O75822) [Homo sapiens]
          Length = 226

 Score = 29.1 bits (62), Expect = 8.4
 Identities = 18/60 (30%), Positives = 29/60 (48%)
 Frame = +1

Query: 298 KIKPFVKGVNYNHLMPTRYSVDXSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 477
           +IKP+ K  +Y  L+ T   +  S     A D+KD A       N +++ E+   +GK K
Sbjct: 139 RIKPYEKSYHYIALLKTIMRL--SLTNMKAADVKDVASSITTIANEKLKAEKEAAAGKKK 196


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,732,157
Number of Sequences: 28952
Number of extensions: 432789
Number of successful extensions: 976
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 972
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4221703296
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)