BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_C01_e291_05.seq (1563 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g24290.2 68418.m02858 integral membrane family protein contai... 35 0.13 At5g24290.1 68418.m02857 integral membrane family protein contai... 35 0.13 At1g59550.1 68414.m06696 UBX domain-containing protein contains ... 31 2.7 At3g18800.1 68416.m02388 expressed protein 30 3.6 At5g39420.1 68418.m04775 protein kinase family protein contains ... 30 4.8 At5g58120.1 68418.m07272 disease resistance protein (TIR-NBS-LRR... 29 6.3 At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 29 8.3 At2g16290.1 68415.m01865 F-box family protein contains Pfam PF00... 29 8.3 At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 29 8.3 >At5g24290.2 68418.m02858 integral membrane family protein contains Pfam domain PF01988: Integral membrane protein Length = 534 Score = 35.1 bits (77), Expect = 0.13 Identities = 27/120 (22%), Positives = 54/120 (45%), Gaps = 7/120 (5%) Frame = +2 Query: 269 EANFKTPIDRGLDESHFRKLFLRRKVVMLRKRDLERSKGAEPATVTVEELRDYNYNNEND 448 E N + + ++S+ KL ++ L D E+++ + + L + EN Sbjct: 134 EENNERSLSDSEEKSNLEKLLGTQENYELGNEDEEKNERSSSDSEEKSNLENLLATQENY 193 Query: 449 ASYCPS-------NMPNNDDWSNEDASAAIDLHEQVKLAEPDEITDMLEPPTKVAKIFIP 607 YCPS N+ + ++++DL + + EPDE +D+ E + V K+++P Sbjct: 194 ELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDIPVVEPDEPSDIEEMESPV-KVYVP 252 >At5g24290.1 68418.m02857 integral membrane family protein contains Pfam domain PF01988: Integral membrane protein Length = 550 Score = 35.1 bits (77), Expect = 0.13 Identities = 27/120 (22%), Positives = 54/120 (45%), Gaps = 7/120 (5%) Frame = +2 Query: 269 EANFKTPIDRGLDESHFRKLFLRRKVVMLRKRDLERSKGAEPATVTVEELRDYNYNNEND 448 E N + + ++S+ KL ++ L D E+++ + + L + EN Sbjct: 150 EENNERSLSDSEEKSNLEKLLGTQENYELGNEDEEKNERSSSDSEEKSNLENLLATQENY 209 Query: 449 ASYCPS-------NMPNNDDWSNEDASAAIDLHEQVKLAEPDEITDMLEPPTKVAKIFIP 607 YCPS N+ + ++++DL + + EPDE +D+ E + V K+++P Sbjct: 210 ELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDIPVVEPDEPSDIEEMESPV-KVYVP 268 >At1g59550.1 68414.m06696 UBX domain-containing protein contains Pfam profile PF00789: UBX domain Length = 307 Score = 30.7 bits (66), Expect = 2.7 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +2 Query: 461 PSNMPNNDDWSNEDASAAIDLHEQVK 538 P N N D WSNED S A++ ++K Sbjct: 107 PCNTFNRDLWSNEDVSQALEFRGEIK 132 >At3g18800.1 68416.m02388 expressed protein Length = 197 Score = 30.3 bits (65), Expect = 3.6 Identities = 10/19 (52%), Positives = 13/19 (68%) Frame = -2 Query: 803 SRAEPAGRSSCRACWTTIC 747 S ++P+GR CR CW IC Sbjct: 67 SLSQPSGRLHCRHCWAPIC 85 >At5g39420.1 68418.m04775 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 644 Score = 29.9 bits (64), Expect = 4.8 Identities = 19/67 (28%), Positives = 28/67 (41%) Frame = +2 Query: 101 DHGMRGDRRGTKRQLDFTGAGLAFDCRAEPAGEYEPTQPAKIMISRKTLATGHTWNEANF 280 D R D R + L +G+ + EY+P AK+ I + TL + NEA+ Sbjct: 411 DAKYRDDMRRKRANLKLRDSGVGRKHKRPHRAEYDPKNYAKLPIRKDTLEVKNIPNEASR 470 Query: 281 KTPIDRG 301 T G Sbjct: 471 ATTTTHG 477 >At5g58120.1 68418.m07272 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1046 Score = 29.5 bits (63), Expect = 6.3 Identities = 22/69 (31%), Positives = 30/69 (43%), Gaps = 4/69 (5%) Frame = +2 Query: 227 MISRKTLATGHTWNE---ANFKTPIDRGLDESHFRKLFLRRKVVMLRKRDLERSKGAEPA 397 M S + + TW NF P R SH RK F + M + +ERS+ PA Sbjct: 1 MASSSSSSRSRTWRYRVFTNFHGPDVRKTFLSHLRKQFSYNGISMFNDQSIERSQTIVPA 60 Query: 398 -TVTVEELR 421 T ++E R Sbjct: 61 LTGAIKESR 69 >At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to 4CL2 [gi:12229665] from Arabidopsis thaliana, 4CL1 [gi:12229631] from Nicotiana tabacum; contains Pfam AMP-binding enzyme domain PF00501 Length = 562 Score = 29.1 bits (62), Expect = 8.3 Identities = 17/58 (29%), Positives = 30/58 (51%) Frame = -1 Query: 828 PQAVLPMAFESRASGTLKLSRMLDDNLLGSREYTLKNEVAATVSFSPAGVFSVNIFHV 655 P A+L F S +G +K + NL+ S + + + V++ G+FS+ +FHV Sbjct: 206 PAAIL---FSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNYDRVGLFSLPLFHV 260 >At2g16290.1 68415.m01865 F-box family protein contains Pfam PF00646: F-box domain; Length = 415 Score = 29.1 bits (62), Expect = 8.3 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = -2 Query: 368 DPSCVTSPLSASRIIYGNATRPVLY 294 +P + SPLS++RIIYG VL+ Sbjct: 111 EPKLLLSPLSSNRIIYGMGINKVLF 135 >At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative similar to GTL1 [Arabidopsis thaliana] GI:2664198 Length = 669 Score = 29.1 bits (62), Expect = 8.3 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 2/30 (6%) Frame = -2 Query: 719 TKSPPQFPFPR--PVFSPSTFSTCNSSTVS 636 T +PP P P P+F+ TFS+ +SST S Sbjct: 200 TPTPPPLPLPSMGPIFTGVTFSSHSSSTAS 229 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,391,878 Number of Sequences: 28952 Number of extensions: 444826 Number of successful extensions: 1555 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1479 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1555 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 4202426112 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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