BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_B10_e362_04.seq (1480 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Sc... 31 0.53 SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 29 1.2 SPAC11E3.06 |map1||MADS-box transcription factor Map1|Schizosacc... 29 1.6 SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr... 29 2.2 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 29 2.2 SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 29 2.2 SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 27 5.0 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 27 5.0 SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 27 5.0 SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 27 5.0 SPAC4F10.08 |mug126||sequence orphan|Schizosaccharomyces pombe|c... 27 6.6 SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 27 8.7 >SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 776 Score = 30.7 bits (66), Expect = 0.53 Identities = 23/88 (26%), Positives = 38/88 (43%), Gaps = 4/88 (4%) Frame = +3 Query: 699 HTVEVIKEQNGSAYKIKTNINFEDIPNKIILGKTTNGKRLVAKVVKSRKP----MTFLKD 866 H + ++E A K + + NKII + N K + S + +T K Sbjct: 386 HLLRELEELRHLATSAKEDFVKVEKLNKIIKDENENLKEYIRNNSLSFQATAGELTQCKQ 445 Query: 867 YPKPKLSGTDLSNSQFIGVLNTXYVLMG 950 P+P+++G D NS + G N L+G Sbjct: 446 LPQPRITGNDQENSHYTGAGNNCLELVG 473 >SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 29.5 bits (63), Expect = 1.2 Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 7/79 (8%) Frame = +3 Query: 651 QDKVNLNASSNDVS-MDHTVEVIKEQNGSAYK-IKTNINFEDIP-NKIILGKTTNGK--- 812 QD + +N + D + + V Q Y +K N + P +K+ +G TN + Sbjct: 307 QDVIPINGNIPDPAHVKDNVRAASIQRSLEYMGLKPNTSIVSYPIDKVFIGSCTNSRIED 366 Query: 813 -RLVAKVVKSRKPMTFLKD 866 RL A VVK RK +KD Sbjct: 367 LRLAAAVVKGRKVAANVKD 385 >SPAC11E3.06 |map1||MADS-box transcription factor Map1|Schizosaccharomyces pombe|chr 1|||Manual Length = 398 Score = 29.1 bits (62), Expect = 1.6 Identities = 24/95 (25%), Positives = 42/95 (44%) Frame = +3 Query: 564 ENLKIEMDNITDATENHAECCEKQEIVFQQDKVNLNASSNDVSMDHTVEVIKEQNGSAYK 743 E K+ +++ +AT + E Q +Q L+ S + +DH I E+NG ++ Sbjct: 87 EGQKLITESLINATTDQNESQASQA---KQSSAQLSDSESGYPLDHEEMRISEENGPSHI 143 Query: 744 IKTNINFEDIPNKIILGKTTNGKRLVAKVVKSRKP 848 N F DI N T+ + + +K+ S P Sbjct: 144 ENLNF-FSDIDN----FSKTSAEEIASKLFSSVSP 173 >SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 568 Score = 28.7 bits (61), Expect = 2.2 Identities = 31/126 (24%), Positives = 57/126 (45%), Gaps = 8/126 (6%) Frame = +3 Query: 540 DTTFDNGNENLKIEMD-------NITDATENHAECCEKQEIVFQQDKVNLNASSNDVSMD 698 +TT NG E++KI+ D + +A + ++ E++ + + + SN++ D Sbjct: 40 ETTSSNGAEDIKIQRDVGDDKDLDDKEANDKSSKGLEEESDSKDRKTIETDQPSNNIG-D 98 Query: 699 HTVEVIKEQNGSAYK-IKTNINFEDIPNKIILGKTTNGKRLVAKVVKSRKPMTFLKDYPK 875 + ++ NG+A K K + P +L K + + VV K MT + K Sbjct: 99 IKSQKSEKSNGNARKETKQSERVNYKPGMRVLTKMSGFPWWPSMVVTESK-MTSVARKSK 157 Query: 876 PKLSGT 893 PK +GT Sbjct: 158 PKRAGT 163 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 28.7 bits (61), Expect = 2.2 Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Frame = -1 Query: 373 FSFDSKSIQPFLSKVLYWSSLIVTSAQSPDCLLLSLTPVTLFISEFSGV-NSYIATSSIL 197 F F S S Q V+ SS I + C L TP++ F++ +S V +SY+ T+ L Sbjct: 984 FKFKSLSYQREKMGVVELSSNISLLSVENICKQLQTTPLSFFLTAWSTVLSSYLKTNDFL 1043 Query: 196 SSCVL 182 V+ Sbjct: 1044 VGTVV 1048 >SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 28.7 bits (61), Expect = 2.2 Identities = 18/55 (32%), Positives = 25/55 (45%), Gaps = 2/55 (3%) Frame = +3 Query: 408 ITTNDKLIQKL--RNESNNFTEIDANSIGRAETVPNINHVEYYNQYDTTFDNGNE 566 ITTN+K + NES N T I+ G V N+N+ + D +N E Sbjct: 52 ITTNEKQSSPVDSHNESPNDTTINKGEDGNENEVDNVNNNDKKEDEDNVEENEEE 106 >SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 27.5 bits (58), Expect = 5.0 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = -1 Query: 319 SSLIVTSAQSPDCLLLSLTPVTLFISEFSGVNSYIATSSILS 194 SSL S+ S D L S+TP T+ S S V++ A+++I S Sbjct: 167 SSLASLSSASTDNLAASVTPTTVAASVASSVDTDSASATISS 208 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 27.5 bits (58), Expect = 5.0 Identities = 26/100 (26%), Positives = 43/100 (43%) Frame = +3 Query: 171 DVNTNTQEERIEDVAIYELTPENSLINNVTGVNESNKQSGDCAEVTISDDQYKTFERNGW 350 DV + T E D IYE+ + ++G+ + S D A V + D FER Sbjct: 244 DVASTTFES---DKKIYEIGDAPGHRDFISGMI-AGASSADFA-VLVVDSSQNNFERGFL 298 Query: 351 ILLESNEKIFLLNTLGLHDITTNDKLIQKLRNESNNFTEI 470 ++ E +LL LG+ +I + + + + F EI Sbjct: 299 ENGQTREHAYLLRALGISEIVVSVNKLDLMSWSEDRFQEI 338 >SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 766 Score = 27.5 bits (58), Expect = 5.0 Identities = 13/39 (33%), Positives = 21/39 (53%) Frame = +3 Query: 60 LQXIRTRLYGNLFQFQXEYILPSTDSNVYTNSYLLESDV 176 LQ + +YGN ++ + E++L S V T + SDV Sbjct: 152 LQIVMFNIYGNQYESREEHLLLSLFQMVLTTEFEATSDV 190 >SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit Bgs4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1955 Score = 27.5 bits (58), Expect = 5.0 Identities = 13/41 (31%), Positives = 24/41 (58%) Frame = +3 Query: 105 QXEYILPSTDSNVYTNSYLLESDVNTNTQEERIEDVAIYEL 227 + EYI +TD ++ + Y S + + TQ ER + +A++ L Sbjct: 334 EDEYIDVNTDDSLESAEYRWRSHMRSMTQFERAQQIALWLL 374 >SPAC4F10.08 |mug126||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 436 Score = 27.1 bits (57), Expect = 6.6 Identities = 25/98 (25%), Positives = 45/98 (45%), Gaps = 10/98 (10%) Frame = +3 Query: 111 EYILPSTDSNVYTNSYLLESDVNTNTQEERIEDVA----IYELTPENSLINNVTGVN--E 272 E + +T S ++ Y+LE++ N QEE ED + I + E+ NV N E Sbjct: 3 EMLNENTSSTSLSSFYVLENETIFNVQEEMEEDGSESGNIQKTLLEDPCPENVNDENEDE 62 Query: 273 SNKQSGDCAEVTISDDQYKTFER----NGWILLESNEK 374 N+ S + + + +++ + +IL +S EK Sbjct: 63 QNRSSRSSSPLVKHEQRHRMYNLKEVIKSFILTKSKEK 100 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 26.6 bits (56), Expect = 8.7 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = +2 Query: 803 EWEKISRKGGKIKETNDISERLSXTQIKWDR 895 E EK +K K+KET +E L+ T+ W R Sbjct: 244 ESEKKEKKTKKVKETTTETEELNKTKPIWTR 274 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,831,306 Number of Sequences: 5004 Number of extensions: 93094 Number of successful extensions: 287 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 266 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 287 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 824584384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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