Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_B10_e362_04.seq
(1480 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g50380.1 68416.m05511 expressed protein 31 1.9
At3g07040.1 68416.m00836 disease resistance protein RPM1 (CC-NBS... 31 1.9
At1g72360.1 68414.m08370 ethylene-responsive element-binding pro... 31 2.6
At5g62550.1 68418.m07850 expressed protein 30 3.4
At5g62110.1 68418.m07796 hypothetical protein 30 3.4
At5g55380.1 68418.m06900 membrane bound O-acyl transferase (MBOA... 30 4.5
At5g22800.1 68418.m02666 aminoacyl-tRNA synthetase family protei... 30 4.5
At5g07980.1 68418.m00928 dentin sialophosphoprotein-related cont... 29 5.9
At2g20320.1 68415.m02373 DENN (AEX-3) domain-containing protein ... 29 5.9
At3g20660.1 68416.m02615 organic cation transporter family prote... 29 7.8
At2g38770.1 68415.m04760 expressed protein 29 7.8
At1g49900.1 68414.m05596 zinc finger (C2H2 type) family protein ... 29 7.8
>At3g50380.1 68416.m05511 expressed protein
Length = 3071
Score = 31.1 bits (67), Expect = 1.9
Identities = 17/67 (25%), Positives = 37/67 (55%)
Frame = +3
Query: 351 ILLESNEKIFLLNTLGLHDITTNDKLIQKLRNESNNFTEIDANSIGRAETVPNINHVEYY 530
I L ++IFLL T+ + + +++ I L E++ T ++ +SIG+ T+ + +++Y
Sbjct: 2036 IALREQKEIFLLPTVQVSNFLSSEAAI--LLTETDQNTSMERHSIGKHATIQSGKTIDFY 2093
Query: 531 NQYDTTF 551
D +
Sbjct: 2094 VNPDMIY 2100
>At3g07040.1 68416.m00836 disease resistance protein RPM1 (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein. Identical to
RPM1 (gi:1361985)
Length = 926
Score = 31.1 bits (67), Expect = 1.9
Identities = 29/117 (24%), Positives = 52/117 (44%), Gaps = 7/117 (5%)
Frame = +3
Query: 312 SDDQYKTFERNGWILLESNEKIFLLNTLGLHDITTNDKLIQKLRNESNNFTEIDANSIGR 491
S D+ + E + I S EK+FL L + + +Q L +++ N+I
Sbjct: 741 SIDEEEPLEIDDLIATASIEKLFLAGKL--ERVPSWFNTLQNLTYLGLRGSQLQENAILS 798
Query: 492 AETVPNINHVEYYNQY---DTTFDNGNENLKI----EMDNITDATENHAECCEKQEI 641
+T+P + + +YN Y F G +NLKI +M ++T+ E Q++
Sbjct: 799 IQTLPRLVWLSFYNAYMGPRLRFAQGFQNLKILEIVQMKHLTEVVIEDGAMFELQKL 855
>At1g72360.1 68414.m08370 ethylene-responsive element-binding
protein, putative contains Pfam profile: PF00847 AP2
domain; similar to ethylene responsive element binding
protein (GI:18496063)[Fagus sylvatica]
Length = 211
Score = 30.7 bits (66), Expect = 2.6
Identities = 21/80 (26%), Positives = 36/80 (45%)
Frame = +3
Query: 360 ESNEKIFLLNTLGLHDITTNDKLIQKLRNESNNFTEIDANSIGRAETVPNINHVEYYNQY 539
E+N L++T L DI D + N+S +DA+ + E + N EY++Q
Sbjct: 126 ENNPDTLLIDTQWLEDIIMGDANKKHEPNDSEEANNVDASLLSE-ELLAFENQTEYFSQ- 183
Query: 540 DTTFDNGNENLKIEMDNITD 599
F GN + + ++ D
Sbjct: 184 -MPFTEGNCDSSTSLSSLFD 202
>At5g62550.1 68418.m07850 expressed protein
Length = 487
Score = 30.3 bits (65), Expect = 3.4
Identities = 30/155 (19%), Positives = 57/155 (36%), Gaps = 3/155 (1%)
Frame = +3
Query: 387 NTLGLHDITTNDKLIQKLRNESNNFTEIDANSIGRAETVPNINHVEYYNQYDTTFDN-GN 563
N GL D + + ESN + +S+ ET ++N+ N ++F GN
Sbjct: 194 NMDGLADAMKELSELYNISEESNQVQVSETSSVVAEETAMSLNN---DNDVQSSFSTPGN 250
Query: 564 ENLKIEMDNITDATENHAECCEKQEIVFQQDKVNLNASSNDVSMDHTVEVIKEQNGSAY- 740
N+ E+ ++ K++ Q V D D +V ++ G+
Sbjct: 251 SNITSEITKDDALQDSTVTKTTKEKDALQDSSVTETTKEKDALQDSSVTETSKEEGALQD 310
Query: 741 -KIKTNINFEDIPNKIILGKTTNGKRLVAKVVKSR 842
+ ED + +TT ++ + V + R
Sbjct: 311 SSVTETTKEEDALQDSSVTETTKEEQALETVTQGR 345
>At5g62110.1 68418.m07796 hypothetical protein
Length = 691
Score = 30.3 bits (65), Expect = 3.4
Identities = 21/82 (25%), Positives = 36/82 (43%)
Frame = +3
Query: 246 INNVTGVNESNKQSGDCAEVTISDDQYKTFERNGWILLESNEKIFLLNTLGLHDITTNDK 425
I ++ ++ +N Q + V FERN + S E I L+ ++D + +
Sbjct: 489 IEDINNLSINNHQGQVLSGVNFEFANDNQFERNAILAGYSIEDINNLSINNVNDNSNEET 548
Query: 426 LIQKLRNESNNFTEIDANSIGR 491
L N NFT +D N+I +
Sbjct: 549 LNSIAANNEMNFTILDVNTINQ 570
>At5g55380.1 68418.m06900 membrane bound O-acyl transferase (MBOAT)
family protein / wax synthase-related similar to wax
synthase [gi:5020219] from Simmondsia chinensis
Length = 341
Score = 29.9 bits (64), Expect = 4.5
Identities = 22/78 (28%), Positives = 41/78 (52%), Gaps = 3/78 (3%)
Frame = -1
Query: 511 MLGTVSALPILFASISVKLLLSFLNFCINLSLVVIS*RPNVFNRNIFSFDSKSIQPF--- 341
+L +S LP+ + + L LS ++FC +S++ +S N F +F+FD + P
Sbjct: 33 VLRLLSILPVCILFLVLPLFLSCVHFCA-ISVLFLSWLAN-FKLLLFAFDEGPLFPLPPK 90
Query: 340 LSKVLYWSSLIVTSAQSP 287
LS+ + ++ L + Q P
Sbjct: 91 LSRFICFACLPIKIRQDP 108
>At5g22800.1 68418.m02666 aminoacyl-tRNA synthetase family protein
contains Pfam profiles: PF01411 tRNA synthetases class
II (A), PF02272 DHHA1 domain
Length = 978
Score = 29.9 bits (64), Expect = 4.5
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +3
Query: 474 ANSIGRAETVPNINHVEYYNQYDTTFDNGNENLKIEMDNITDATENHAE 620
A+ I R E V +EY N D+ LK++ +++T+ EN E
Sbjct: 781 ASGIRRIEAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLE 829
>At5g07980.1 68418.m00928 dentin sialophosphoprotein-related
contains weak similarity to Swiss-Prot:Q9NZW4 dentin
sialophosphoprotein precursor [Homo sapiens]
Length = 1501
Score = 29.5 bits (63), Expect = 5.9
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +3
Query: 183 NTQEERIEDVAIYELTPENSLINNVTGVNESN 278
N+ R+E + + +PENS NVTG++E+N
Sbjct: 780 NSASSRLELLHKVDPSPENSSETNVTGIHEAN 811
>At2g20320.1 68415.m02373 DENN (AEX-3) domain-containing protein
contains Pfam domain PF02141: DENN (AEX-3) domain
Length = 976
Score = 29.5 bits (63), Expect = 5.9
Identities = 20/94 (21%), Positives = 39/94 (41%), Gaps = 4/94 (4%)
Frame = +3
Query: 513 NHVEYYNQYDTTFDNGNENLKIEMDNITDATENHAECCEK----QEIVFQQDKVNLNASS 680
NH++ + ++DNG+ + ++ + E CE V D L+ S
Sbjct: 657 NHLDTPERTSQSYDNGHTSPEVTCSDPRTQPIERNESCESVFSSARSVLSDDVDELSNSE 716
Query: 681 NDVSMDHTVEVIKEQNGSAYKIKTNINFEDIPNK 782
ND D +E K+ N + ++ + IP++
Sbjct: 717 NDFGDDLILEWAKDHNNDSLQLVCGYHSLAIPSR 750
>At3g20660.1 68416.m02615 organic cation transporter family protein
similar to organic cation transporter OCT3 [Rattus
norvegicus] GI:3273722, organic cation transporter 3
[Mus musculus] GI:4454795; contains Pfam profile
PF00083: major facilitator superfamily protein
Length = 534
Score = 29.1 bits (62), Expect = 7.8
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = -3
Query: 638 FLFFTAFCMILSSISYIIHFYLEIFI-TIVKCCIILVIIFDMIN 510
F FF+A +LS I+Y+ + E+FI + + + L+I+ I+
Sbjct: 226 FYFFSAGIAVLSGIAYVFRSWRELFIVSSLPSLLFLLIVIPFIS 269
>At2g38770.1 68415.m04760 expressed protein
Length = 1509
Score = 29.1 bits (62), Expect = 7.8
Identities = 15/36 (41%), Positives = 21/36 (58%)
Frame = +3
Query: 279 KQSGDCAEVTISDDQYKTFERNGWILLESNEKIFLL 386
K S AEVT S Y+T R+ W L+ ++ +FLL
Sbjct: 576 KPSSVTAEVTFSIKSYRTQIRSEWNSLKEHDVLFLL 611
>At1g49900.1 68414.m05596 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 917
Score = 29.1 bits (62), Expect = 7.8
Identities = 17/82 (20%), Positives = 42/82 (51%)
Frame = +3
Query: 366 NEKIFLLNTLGLHDITTNDKLIQKLRNESNNFTEIDANSIGRAETVPNINHVEYYNQYDT 545
+++I L + L + +K + N+ N+F E+ I ++++ P YY+ D
Sbjct: 588 SDQISFLESNRLEEEFEEEKEAARFSND-NDFVELLFQGIEQSKSKPKPKSCSYYDDDDD 646
Query: 546 TFDNGNENLKIEMDNITDATEN 611
D+G+E+ + D++ ++++
Sbjct: 647 DDDDGDEDDDDDDDDVDPSSKD 668
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,552,811
Number of Sequences: 28952
Number of extensions: 478494
Number of successful extensions: 1199
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1198
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3932545536
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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