Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_B09_e354_03.seq
(1496 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 268 1e-72
SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|... 266 4e-72
SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom... 230 3e-61
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 31 0.41
SPAC1805.06c |hem2||porphobilinogen synthase Hem2 |Schizosacchar... 29 1.3
SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces... 29 2.2
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 28 2.9
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 5.1
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 27 5.1
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 27 6.7
>SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 268 bits (657), Expect = 1e-72
Identities = 123/242 (50%), Positives = 169/242 (69%), Gaps = 1/242 (0%)
Frame = +3
Query: 174 SKKLPAVPESVXXXXXXXXXXXXXXXQITLKRRSASIKKRKEIFKRAEQYVKEYRIKERD 353
SK+ PES+ ++++A KKR+ I KRAE Y EYR ER+
Sbjct: 10 SKEQIFAPESLLKKKKTQEQSREQRVAAAAEKKAAQQKKRELIAKRAESYDAEYRKAERE 69
Query: 354 EIRLARQARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNK 533
+I L R+AR GNYYVP E KL FVIRIRG+N + PK RK++QL RL QINNGVFV+ NK
Sbjct: 70 QIELGRKARAEGNYYVPDETKLVFVIRIRGINNIPPKARKIMQLLRLIQINNGVFVKFNK 129
Query: 534 ATVNMLRIAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICV 713
AT ML++ EPY+ +G PNLK+VREL+YKRGF K+N +R+ ++ N++IE L K +I+ +
Sbjct: 130 ATKEMLQVVEPYVTYGIPNLKTVRELLYKRGFGKVNKQRIALSDNAIIEAALGKYSILSI 189
Query: 714 EDLIHEIFTVGEKFKYASNFLWPFXLNNPTGGWR-KKTIHYVDGGDFGNREDKVXELLXR 890
EDLIHEI+TVG FK A+NF+WPF L++P GGWR +K H+++GGD G R++ + L+ +
Sbjct: 190 EDLIHEIYTVGPNFKQAANFIWPFQLSSPLGGWRDRKFKHFIEGGDAGKRDEHINSLVRK 249
Query: 891 MV 896
M+
Sbjct: 250 ML 251
>SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 250
Score = 266 bits (653), Expect = 4e-72
Identities = 121/235 (51%), Positives = 166/235 (70%), Gaps = 1/235 (0%)
Frame = +3
Query: 195 PESVXXXXXXXXXXXXXXXQITLKRRSASIKKRKEIFKRAEQYVKEYRIKERDEIRLARQ 374
PES+ +++SA KKR+ I KRAE Y EYR ER++I LAR+
Sbjct: 16 PESLLKKTKAQKQSREQIVAAAAEKKSARQKKRELIAKRAEAYEAEYRAAEREQIELARK 75
Query: 375 ARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLR 554
AR GNY+VP E KL FV+RIRG+N + PK RK++QL RL QINNG+FV+ NKA ML+
Sbjct: 76 ARAEGNYFVPHEPKLIFVVRIRGINNIPPKARKIMQLLRLLQINNGIFVKFNKAIKEMLQ 135
Query: 555 IAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEI 734
+ EPY+ +G PN K+VREL+YKRGF K+N +R+P++ N++IE L K +I+ VEDLIHEI
Sbjct: 136 VVEPYVTYGIPNHKTVRELIYKRGFGKVNKQRIPLSDNAIIEAALGKYSILSVEDLIHEI 195
Query: 735 FTVGEKFKYASNFLWPFXLNNPTGGWR-KKTIHYVDGGDFGNREDKVXELLXRMV 896
+TVG FK A+NFLWPF L++P GGWR +K H+++GGD G R++ + L+ +M+
Sbjct: 196 YTVGPNFKQAANFLWPFKLSSPLGGWRERKFKHFIEGGDAGKRDEHINGLVQKML 250
>SPAC664.06 |rpl703|rpl7|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 249
Score = 230 bits (563), Expect = 3e-61
Identities = 108/213 (50%), Positives = 146/213 (68%), Gaps = 1/213 (0%)
Frame = +3
Query: 261 LKRRSASIKKRKEIFKRAEQYVKEYRIKERDEIRLARQARNRGNYYVPGEAKLAFVIRIR 440
+ ++ A K RKE FKRAE ++ YR +ER+ IRL R A+N+G+ +VP E KL FVIRI
Sbjct: 37 IAKKEAQKKNRKETFKRAETFINNYRQRERERIRLNRSAKNKGDIFVPDETKLLFVIRIA 96
Query: 441 GVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNLKSVRELVYK 620
GV + PK+RKVL+L RL +INN VFVR NKA MLRI EPY+ +G PNL SVREL+YK
Sbjct: 97 GVKNMPPKIRKVLRLLRLSRINNAVFVRNNKAVAQMLRIVEPYVMYGIPNLHSVRELIYK 156
Query: 621 RGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEIFTVGEKFKYASNFLWPFXLNNP 800
RGF K+NG+R+ ++ N+LIE+ L K ++I +ED+IHEI+ VG FK + FLWPF L
Sbjct: 157 RGFGKINGQRIALSDNALIEEALGKYDVISIEDIIHEIYNVGSHFKEVTKFLWPFTLTPV 216
Query: 801 TGG-WRKKTIHYVDGGDFGNREDKVXELLXRMV 896
KK H+ +G G +++ EL+ + V
Sbjct: 217 KHSLMEKKVKHFNEGRKAGYCGEEINELIKKQV 249
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 31.1 bits (67), Expect = 0.41
Identities = 15/35 (42%), Positives = 25/35 (71%)
Frame = +1
Query: 670 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTS 774
HSS + L+N+ SSAL+++ + S +SSS+Q +S
Sbjct: 90 HSSSQKLSNKVSSALKLTIPKRWRSSKSSSSQCSS 124
>SPAC1805.06c |hem2||porphobilinogen synthase Hem2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 329
Score = 29.5 bits (63), Expect = 1.3
Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 2/68 (2%)
Frame = +3
Query: 588 NLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLS--KQNIICVEDLIHEIFTVGEKFKY 761
N KSV + G L G ++ I+ + ++ R+ KQ ++ +E L H++ + K+
Sbjct: 143 NAKSVERIAEVSGNYALAGAQI-ISPSDCMDGRVKAIKQKLVELE-LSHKVCVISYSAKF 200
Query: 762 ASNFLWPF 785
AS F PF
Sbjct: 201 ASGFFGPF 208
>SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 224
Score = 28.7 bits (61), Expect = 2.2
Identities = 11/38 (28%), Positives = 24/38 (63%)
Frame = +3
Query: 279 SIKKRKEIFKRAEQYVKEYRIKERDEIRLARQARNRGN 392
S+K+ +EI ++ E+ +R+K ++ ++ + A N GN
Sbjct: 152 SLKRNREIIEKEERSSFHFRVKPKNLDKVPKLAENEGN 189
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 28.3 bits (60), Expect = 2.9
Identities = 29/100 (29%), Positives = 48/100 (48%)
Frame = +1
Query: 475 YCSCSGCVRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVS 654
Y + +G +++T + S VS+ + S SP T T S S +++ S + + S S
Sbjct: 116 YLTGNGVLQTTVSSSSVSSTTSSSS--SSSPSSSSTTTTTSPSSSSSSSSSSSS-SSSSS 172
Query: 655 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTS 774
S + SS ++ +SS+ SS S SSS +TS
Sbjct: 173 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITS 212
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.5 bits (58), Expect = 5.1
Identities = 32/108 (29%), Positives = 50/108 (46%), Gaps = 7/108 (6%)
Frame = +1
Query: 496 VRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TG-------SVS 654
V STTA S ST P T S + +TP V+ + T+A S T SVS
Sbjct: 460 VNSTTATSASST-PL-TSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVS 517
Query: 655 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSX*TT 798
+ P++++ L + S+ +SS + +SST +TS + T+
Sbjct: 518 STAPSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATS 565
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 27.5 bits (58), Expect = 5.1
Identities = 16/40 (40%), Positives = 24/40 (60%)
Frame = +3
Query: 375 ARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRL 494
+ N+G YY G +AFV I GV + SP++ LQL ++
Sbjct: 1133 SENKGMYY--GLLGIAFVA-IAGVTEFSPELNAKLQLVKM 1169
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 27.1 bits (57), Expect = 6.7
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +3
Query: 585 PNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNII 707
P LK+ REL+ K+ + V SN ++EK L +II
Sbjct: 423 PKLKAERELLSKQESMEKQSMHVDEESNQILEKFLDVYDII 463
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,524,162
Number of Sequences: 5004
Number of extensions: 81839
Number of successful extensions: 238
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 836477428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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