BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_A12_e377_02.seq (1488 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 30 0.94 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 2.2 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 3.8 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 6.6 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 8.8 >SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1|||Manual Length = 881 Score = 29.9 bits (64), Expect = 0.94 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%) Frame = -1 Query: 372 SNLISIIC--XLQFGFFFLCHLIVFLLSVQKPPNIILYRRLC 253 SN+I +IC F F+C++ +LS P+ I+Y +LC Sbjct: 197 SNVIWLICFGAPLFLVIFICYIFFTVLSFFNVPDAIVYSKLC 238 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 28.7 bits (61), Expect = 2.2 Identities = 9/18 (50%), Positives = 9/18 (50%) Frame = -3 Query: 1474 PPXGGXPPPXPXPXPGXP 1421 PP PPP P P P P Sbjct: 470 PPAAPAPPPAPAPAPAAP 487 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.9 bits (59), Expect = 3.8 Identities = 13/36 (36%), Positives = 15/36 (41%), Gaps = 2/36 (5%) Frame = -2 Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPG--PXXPXPP 1374 PP+ P P P P + P P P P P PP Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.1 bits (57), Expect = 6.6 Identities = 14/47 (29%), Positives = 18/47 (38%) Frame = +3 Query: 1017 PXXPQGXARGXTXXPPXSXAXSAXXTGXAXPXGPIPPGXPXGXKPIP 1157 P P+ + G PP A S+ P G P P G P+P Sbjct: 1050 PPVPKSSS-GAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVP 1095 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 26.6 bits (56), Expect = 8.8 Identities = 11/25 (44%), Positives = 11/25 (44%) Frame = -2 Query: 1457 PXPXPXPXAGXXPKXGGPXPGPXXP 1383 P P P P P GGP P P P Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPP 768 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,560,366 Number of Sequences: 5004 Number of extensions: 81014 Number of successful extensions: 148 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 125 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 830530906 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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