BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_A12_e377_02.seq (1488 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58160.1 68418.m07280 formin homology 2 domain-containing pro... 33 0.48 At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family... 31 1.5 At2g25460.1 68415.m03049 expressed protein 31 2.6 At2g03260.1 68415.m00279 EXS family protein / ERD1/XPR1/SYG1 fam... 30 3.4 At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 30 4.5 At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH) domain... 24 5.1 At2g46700.1 68415.m05827 calcium-dependent protein kinase, putat... 29 5.9 At1g59910.1 68414.m06749 formin homology 2 domain-containing pro... 29 5.9 At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 29 7.8 At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 29 7.8 At4g19200.1 68417.m02833 proline-rich family protein contains pr... 29 7.8 At4g08230.1 68417.m01358 glycine-rich protein 29 7.8 At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 24 8.4 >At5g58160.1 68418.m07280 formin homology 2 domain-containing protein / FH2 domain-containing protein low similarity to SP|Q05858 Formin (Limb deformity protein) {Gallus gallus}; contains Pfam profile PF02181: Formin Homology 2(FH2) Domain Length = 1307 Score = 33.1 bits (72), Expect = 0.48 Identities = 16/47 (34%), Positives = 17/47 (36%) Frame = -3 Query: 1456 PPPXPXPXPGXPQXXGAXXXGLXFXXPPGFLRXPRXXXNPXQXPLPP 1316 PPP P P P PQ G PP R P +P PP Sbjct: 731 PPPPPPPAPPTPQSNGISAMKSSPPAPPAPPRLPTHSASPPPPTAPP 777 >At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family protein Common family members: At4g18570, At4g04980, At5g61090 [Arabidopsis thaliana]; identical to cDNA CHUP1 for actin binding protein GI:28071264 Length = 1004 Score = 31.5 bits (68), Expect = 1.5 Identities = 16/33 (48%), Positives = 16/33 (48%) Frame = -2 Query: 1472 PRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374 P GG P P P P G P GG P P P PP Sbjct: 675 PGGGPPPPPPPPGGGPPPPPGGGPPPP--PPPP 705 >At2g25460.1 68415.m03049 expressed protein Length = 423 Score = 30.7 bits (66), Expect = 2.6 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -2 Query: 1199 KXPVLXXFLFSXPERDGFSPGRXPGWNW 1116 + PV+ LFS P+ + PG+ G+NW Sbjct: 209 RSPVVETGLFSSPDENQSEPGQKAGFNW 236 >At2g03260.1 68415.m00279 EXS family protein / ERD1/XPR1/SYG1 family protein similar to PHO1 protein [Arabidopsis thaliana] GI:20069032; contains Pfam profiles PF03105: SPX domain, PF03124: EXS family Length = 807 Score = 30.3 bits (65), Expect = 3.4 Identities = 22/85 (25%), Positives = 37/85 (43%), Gaps = 2/85 (2%) Frame = -3 Query: 709 LIMLWLLYKMIFIDFYLPKFYRHDSIRLFSLE--LYCLVYYFYTISVSLIMLGPELNWLC 536 L+ L+LL + F+ +P + + S R F L L+CL Y +++ LG +L Sbjct: 523 LLPLFLLVAL-FVVLIMPFHFLYRSTRFFFLTCLLHCLAAPLYKVTLPDFFLGDQLTSQV 581 Query: 535 GRYYDVKIPICIKXTFSILKKKNWC 461 + IC K++N C Sbjct: 582 QALRSINFYICYYGWGDFKKRQNTC 606 >At1g20130.1 68414.m02518 family II extracellular lipase, putative contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566) Length = 1006 Score = 29.9 bits (64), Expect = 4.5 Identities = 15/39 (38%), Positives = 17/39 (43%), Gaps = 4/39 (10%) Frame = -2 Query: 1475 PPRGGXPXPXPXPXAGXXPK-XGGPXPGPX---XPXPPR 1371 PP+ P P P P G PK P P P P PP+ Sbjct: 101 PPKPPAPTPKPVPPHGPPPKPAPAPTPAPSPKPAPSPPK 139 Score = 29.1 bits (62), Expect = 7.8 Identities = 13/31 (41%), Positives = 13/31 (41%) Frame = -2 Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXP 1383 PP G P P P P PK P P P P Sbjct: 113 PPHGPPPKPAPAPTPAPSPK---PAPSPPKP 140 >At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related Aux22d, Vigna radiata, PID:D1021691; contains Pfam PF01417: ENTH domain. ENTH (Epsin N-terminal homology) domain; similar to clathrin assembly protein AP180 (GI:6492344) [Xenopus laevis] Length = 635 Score = 24.2 bits (50), Expect(2) = 5.1 Identities = 8/15 (53%), Positives = 8/15 (53%) Frame = -3 Query: 1474 PPXGGXPPPXPXPXP 1430 PP PPP P P P Sbjct: 392 PPENHTPPPPPAPEP 406 Score = 23.8 bits (49), Expect(2) = 5.1 Identities = 8/13 (61%), Positives = 8/13 (61%) Frame = -3 Query: 1456 PPPXPXPXPGXPQ 1418 PPP P P P PQ Sbjct: 400 PPPAPEPKPQQPQ 412 >At2g46700.1 68415.m05827 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium/calmodulin-dependent protein kinase homolog MCK1 [Zea mays] gi|1839597|gb|AAB47181 Length = 595 Score = 29.5 bits (63), Expect = 5.9 Identities = 13/35 (37%), Positives = 14/35 (40%), Gaps = 1/35 (2%) Frame = -2 Query: 1457 PXPXPXPXAGXXPKXGGPXPGPX-XPXPPRFFKXP 1356 P P P P P G P P P RFF+ P Sbjct: 56 PWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRP 90 >At1g59910.1 68414.m06749 formin homology 2 domain-containing protein / FH2 domain-containing protein contains formin homology 2 domain, Pfam:PF02128 Length = 929 Score = 29.5 bits (63), Expect = 5.9 Identities = 13/34 (38%), Positives = 13/34 (38%) Frame = -2 Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374 P P P P P A P P GP P PP Sbjct: 378 PANQTSPPPPPPPSAAAPPPPPPPKKGPAAPPPP 411 >At5g54650.2 68418.m06805 formin homology 2 domain-containing protein / FH2 domain-containing protein contains formin homology 2 domain, Pfam:PF02181 Length = 900 Score = 29.1 bits (62), Expect = 7.8 Identities = 17/34 (50%), Positives = 18/34 (52%) Frame = -2 Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374 PPR P P P P +G PK P PGP P PP Sbjct: 385 PPRP--PPPAPPPGSGG-PKPPPP-PGPKGPRPP 414 >At5g54650.1 68418.m06804 formin homology 2 domain-containing protein / FH2 domain-containing protein contains formin homology 2 domain, Pfam:PF02181 Length = 900 Score = 29.1 bits (62), Expect = 7.8 Identities = 17/34 (50%), Positives = 18/34 (52%) Frame = -2 Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374 PPR P P P P +G PK P PGP P PP Sbjct: 385 PPRP--PPPAPPPGSGG-PKPPPP-PGPKGPRPP 414 >At4g19200.1 68417.m02833 proline-rich family protein contains proline rich extensin domains, INTERPRO:IPR002965 Length = 179 Score = 29.1 bits (62), Expect = 7.8 Identities = 14/37 (37%), Positives = 16/37 (43%) Frame = +1 Query: 1366 KNRGGXGXXGPGXGPPXFGXXPAXGXGXGXGXPPRGG 1476 + +G G G G PP G P G G PP GG Sbjct: 12 QEKGFHGFPGGGHYPPAQGGYPPQGYPPQQGYPPAGG 48 >At4g08230.1 68417.m01358 glycine-rich protein Length = 113 Score = 29.1 bits (62), Expect = 7.8 Identities = 14/45 (31%), Positives = 17/45 (37%) Frame = -2 Query: 1175 LFSXPERDGFSPGRXPGWNWAXXXXXPCXXGGXSXRXGGGVGXSP 1041 +FS + D + G P W GG S GGG G P Sbjct: 39 MFSMEKSDAYRKGSKPNKKWGGGMGGGGGGGGGSGGGGGGRGGGP 83 >At4g13340.1 68417.m02084 leucine-rich repeat family protein / extensin family protein similar to extensin-like protein [Lycopersicon esculentum] gi|5917664|gb|AAD55979; contains leucine-rich repeats, Pfam:PF00560; contains proline rich extensin domains, INTERPRO:IPR002965 Length = 760 Score = 24.2 bits (50), Expect(2) = 8.4 Identities = 8/15 (53%), Positives = 8/15 (53%) Frame = -3 Query: 1474 PPXGGXPPPXPXPXP 1430 PP PPP P P P Sbjct: 515 PPVYSSPPPPPSPAP 529 Score = 23.0 bits (47), Expect(2) = 8.4 Identities = 14/47 (29%), Positives = 15/47 (31%) Frame = -3 Query: 1456 PPPXPXPXPGXPQXXGAXXXGLXFXXPPGFLRXPRXXXNPXQXPLPP 1316 PPP P P PQ + PP P P P PP Sbjct: 537 PPPPPPHSPPPPQFSPPPPEPYYYSSPP-----PPHSSPPPHSPPPP 578 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 24,550,628 Number of Sequences: 28952 Number of extensions: 478520 Number of successful extensions: 1695 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 979 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1502 length of database: 12,070,560 effective HSP length: 84 effective length of database: 9,638,592 effective search space used: 3961461312 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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