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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_A12_e377_02.seq
         (1488 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g58160.1 68418.m07280 formin homology 2 domain-containing pro...    33   0.48 
At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family...    31   1.5  
At2g25460.1 68415.m03049 expressed protein                             31   2.6  
At2g03260.1 68415.m00279 EXS family protein / ERD1/XPR1/SYG1 fam...    30   3.4  
At1g20130.1 68414.m02518 family II extracellular lipase, putativ...    30   4.5  
At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH) domain...    24   5.1  
At2g46700.1 68415.m05827 calcium-dependent protein kinase, putat...    29   5.9  
At1g59910.1 68414.m06749 formin homology 2 domain-containing pro...    29   5.9  
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro...    29   7.8  
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro...    29   7.8  
At4g19200.1 68417.m02833 proline-rich family protein contains pr...    29   7.8  
At4g08230.1 68417.m01358 glycine-rich protein                          29   7.8  
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex...    24   8.4  

>At5g58160.1 68418.m07280 formin homology 2 domain-containing protein
            / FH2 domain-containing protein low similarity to
            SP|Q05858 Formin (Limb deformity protein) {Gallus
            gallus}; contains Pfam profile PF02181: Formin Homology
            2(FH2) Domain
          Length = 1307

 Score = 33.1 bits (72), Expect = 0.48
 Identities = 16/47 (34%), Positives = 17/47 (36%)
 Frame = -3

Query: 1456 PPPXPXPXPGXPQXXGAXXXGLXFXXPPGFLRXPRXXXNPXQXPLPP 1316
            PPP P P P  PQ  G          PP   R P    +P     PP
Sbjct: 731  PPPPPPPAPPTPQSNGISAMKSSPPAPPAPPRLPTHSASPPPPTAPP 777


>At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family
            protein Common family members: At4g18570, At4g04980,
            At5g61090 [Arabidopsis thaliana];  identical to cDNA
            CHUP1 for actin binding protein GI:28071264
          Length = 1004

 Score = 31.5 bits (68), Expect = 1.5
 Identities = 16/33 (48%), Positives = 16/33 (48%)
 Frame = -2

Query: 1472 PRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
            P GG P P P P  G  P  GG  P P  P PP
Sbjct: 675  PGGGPPPPPPPPGGGPPPPPGGGPPPP--PPPP 705


>At2g25460.1 68415.m03049 expressed protein
          Length = 423

 Score = 30.7 bits (66), Expect = 2.6
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = -2

Query: 1199 KXPVLXXFLFSXPERDGFSPGRXPGWNW 1116
            + PV+   LFS P+ +   PG+  G+NW
Sbjct: 209  RSPVVETGLFSSPDENQSEPGQKAGFNW 236


>At2g03260.1 68415.m00279 EXS family protein / ERD1/XPR1/SYG1 family
           protein similar to PHO1 protein [Arabidopsis thaliana]
           GI:20069032; contains Pfam profiles PF03105: SPX domain,
           PF03124: EXS family
          Length = 807

 Score = 30.3 bits (65), Expect = 3.4
 Identities = 22/85 (25%), Positives = 37/85 (43%), Gaps = 2/85 (2%)
 Frame = -3

Query: 709 LIMLWLLYKMIFIDFYLPKFYRHDSIRLFSLE--LYCLVYYFYTISVSLIMLGPELNWLC 536
           L+ L+LL  + F+   +P  + + S R F L   L+CL    Y +++    LG +L    
Sbjct: 523 LLPLFLLVAL-FVVLIMPFHFLYRSTRFFFLTCLLHCLAAPLYKVTLPDFFLGDQLTSQV 581

Query: 535 GRYYDVKIPICIKXTFSILKKKNWC 461
                +   IC        K++N C
Sbjct: 582 QALRSINFYICYYGWGDFKKRQNTC 606


>At1g20130.1 68414.m02518 family II extracellular lipase, putative
            contains Pfam profile PF00657: GDSL-like
            Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566)
          Length = 1006

 Score = 29.9 bits (64), Expect = 4.5
 Identities = 15/39 (38%), Positives = 17/39 (43%), Gaps = 4/39 (10%)
 Frame = -2

Query: 1475 PPRGGXPXPXPXPXAGXXPK-XGGPXPGPX---XPXPPR 1371
            PP+   P P P P  G  PK    P P P     P PP+
Sbjct: 101  PPKPPAPTPKPVPPHGPPPKPAPAPTPAPSPKPAPSPPK 139



 Score = 29.1 bits (62), Expect = 7.8
 Identities = 13/31 (41%), Positives = 13/31 (41%)
 Frame = -2

Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXP 1383
            PP G  P P P P     PK   P P P  P
Sbjct: 113  PPHGPPPKPAPAPTPAPSPK---PAPSPPKP 140


>At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH)
            domain-containing protein / clathrin assembly
            protein-related Aux22d, Vigna radiata, PID:D1021691;
            contains Pfam PF01417: ENTH domain. ENTH (Epsin
            N-terminal homology) domain;  similar to clathrin
            assembly protein AP180 (GI:6492344) [Xenopus laevis]
          Length = 635

 Score = 24.2 bits (50), Expect(2) = 5.1
 Identities = 8/15 (53%), Positives = 8/15 (53%)
 Frame = -3

Query: 1474 PPXGGXPPPXPXPXP 1430
            PP    PPP P P P
Sbjct: 392  PPENHTPPPPPAPEP 406



 Score = 23.8 bits (49), Expect(2) = 5.1
 Identities = 8/13 (61%), Positives = 8/13 (61%)
 Frame = -3

Query: 1456 PPPXPXPXPGXPQ 1418
            PPP P P P  PQ
Sbjct: 400  PPPAPEPKPQQPQ 412


>At2g46700.1 68415.m05827 calcium-dependent protein kinase, putative /
            CDPK, putative similar to calcium/calmodulin-dependent
            protein kinase homolog MCK1 [Zea mays]
            gi|1839597|gb|AAB47181
          Length = 595

 Score = 29.5 bits (63), Expect = 5.9
 Identities = 13/35 (37%), Positives = 14/35 (40%), Gaps = 1/35 (2%)
 Frame = -2

Query: 1457 PXPXPXPXAGXXPKXGGPXPGPX-XPXPPRFFKXP 1356
            P P P P     P   G  P P     P RFF+ P
Sbjct: 56   PWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRP 90


>At1g59910.1 68414.m06749 formin homology 2 domain-containing protein
            / FH2 domain-containing protein contains formin homology
            2 domain, Pfam:PF02128
          Length = 929

 Score = 29.5 bits (63), Expect = 5.9
 Identities = 13/34 (38%), Positives = 13/34 (38%)
 Frame = -2

Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
            P     P P P P A   P    P  GP  P PP
Sbjct: 378  PANQTSPPPPPPPSAAAPPPPPPPKKGPAAPPPP 411


>At5g54650.2 68418.m06805 formin homology 2 domain-containing protein
            / FH2 domain-containing protein contains formin homology
            2 domain, Pfam:PF02181
          Length = 900

 Score = 29.1 bits (62), Expect = 7.8
 Identities = 17/34 (50%), Positives = 18/34 (52%)
 Frame = -2

Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
            PPR   P P P P +G  PK   P PGP  P PP
Sbjct: 385  PPRP--PPPAPPPGSGG-PKPPPP-PGPKGPRPP 414


>At5g54650.1 68418.m06804 formin homology 2 domain-containing protein
            / FH2 domain-containing protein contains formin homology
            2 domain, Pfam:PF02181
          Length = 900

 Score = 29.1 bits (62), Expect = 7.8
 Identities = 17/34 (50%), Positives = 18/34 (52%)
 Frame = -2

Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
            PPR   P P P P +G  PK   P PGP  P PP
Sbjct: 385  PPRP--PPPAPPPGSGG-PKPPPP-PGPKGPRPP 414


>At4g19200.1 68417.m02833 proline-rich family protein contains proline
            rich extensin domains, INTERPRO:IPR002965
          Length = 179

 Score = 29.1 bits (62), Expect = 7.8
 Identities = 14/37 (37%), Positives = 16/37 (43%)
 Frame = +1

Query: 1366 KNRGGXGXXGPGXGPPXFGXXPAXGXGXGXGXPPRGG 1476
            + +G  G  G G  PP  G  P  G     G PP GG
Sbjct: 12   QEKGFHGFPGGGHYPPAQGGYPPQGYPPQQGYPPAGG 48


>At4g08230.1 68417.m01358 glycine-rich protein
          Length = 113

 Score = 29.1 bits (62), Expect = 7.8
 Identities = 14/45 (31%), Positives = 17/45 (37%)
 Frame = -2

Query: 1175 LFSXPERDGFSPGRXPGWNWAXXXXXPCXXGGXSXRXGGGVGXSP 1041
            +FS  + D +  G  P   W          GG S   GGG G  P
Sbjct: 39   MFSMEKSDAYRKGSKPNKKWGGGMGGGGGGGGGSGGGGGGRGGGP 83


>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
            extensin family protein similar to extensin-like protein
            [Lycopersicon esculentum] gi|5917664|gb|AAD55979;
            contains leucine-rich repeats, Pfam:PF00560; contains
            proline rich extensin domains, INTERPRO:IPR002965
          Length = 760

 Score = 24.2 bits (50), Expect(2) = 8.4
 Identities = 8/15 (53%), Positives = 8/15 (53%)
 Frame = -3

Query: 1474 PPXGGXPPPXPXPXP 1430
            PP    PPP P P P
Sbjct: 515  PPVYSSPPPPPSPAP 529



 Score = 23.0 bits (47), Expect(2) = 8.4
 Identities = 14/47 (29%), Positives = 15/47 (31%)
 Frame = -3

Query: 1456 PPPXPXPXPGXPQXXGAXXXGLXFXXPPGFLRXPRXXXNPXQXPLPP 1316
            PPP P   P  PQ          +  PP     P     P   P PP
Sbjct: 537  PPPPPPHSPPPPQFSPPPPEPYYYSSPP-----PPHSSPPPHSPPPP 578


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,550,628
Number of Sequences: 28952
Number of extensions: 478520
Number of successful extensions: 1695
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 979
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1502
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3961461312
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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