Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_A12_e377_02.seq
(1488 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58160.1 68418.m07280 formin homology 2 domain-containing pro... 33 0.48
At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family... 31 1.5
At2g25460.1 68415.m03049 expressed protein 31 2.6
At2g03260.1 68415.m00279 EXS family protein / ERD1/XPR1/SYG1 fam... 30 3.4
At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 30 4.5
At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH) domain... 24 5.1
At2g46700.1 68415.m05827 calcium-dependent protein kinase, putat... 29 5.9
At1g59910.1 68414.m06749 formin homology 2 domain-containing pro... 29 5.9
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 29 7.8
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 29 7.8
At4g19200.1 68417.m02833 proline-rich family protein contains pr... 29 7.8
At4g08230.1 68417.m01358 glycine-rich protein 29 7.8
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 24 8.4
>At5g58160.1 68418.m07280 formin homology 2 domain-containing protein
/ FH2 domain-containing protein low similarity to
SP|Q05858 Formin (Limb deformity protein) {Gallus
gallus}; contains Pfam profile PF02181: Formin Homology
2(FH2) Domain
Length = 1307
Score = 33.1 bits (72), Expect = 0.48
Identities = 16/47 (34%), Positives = 17/47 (36%)
Frame = -3
Query: 1456 PPPXPXPXPGXPQXXGAXXXGLXFXXPPGFLRXPRXXXNPXQXPLPP 1316
PPP P P P PQ G PP R P +P PP
Sbjct: 731 PPPPPPPAPPTPQSNGISAMKSSPPAPPAPPRLPTHSASPPPPTAPP 777
>At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family
protein Common family members: At4g18570, At4g04980,
At5g61090 [Arabidopsis thaliana]; identical to cDNA
CHUP1 for actin binding protein GI:28071264
Length = 1004
Score = 31.5 bits (68), Expect = 1.5
Identities = 16/33 (48%), Positives = 16/33 (48%)
Frame = -2
Query: 1472 PRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
P GG P P P P G P GG P P P PP
Sbjct: 675 PGGGPPPPPPPPGGGPPPPPGGGPPPP--PPPP 705
>At2g25460.1 68415.m03049 expressed protein
Length = 423
Score = 30.7 bits (66), Expect = 2.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 1199 KXPVLXXFLFSXPERDGFSPGRXPGWNW 1116
+ PV+ LFS P+ + PG+ G+NW
Sbjct: 209 RSPVVETGLFSSPDENQSEPGQKAGFNW 236
>At2g03260.1 68415.m00279 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 807
Score = 30.3 bits (65), Expect = 3.4
Identities = 22/85 (25%), Positives = 37/85 (43%), Gaps = 2/85 (2%)
Frame = -3
Query: 709 LIMLWLLYKMIFIDFYLPKFYRHDSIRLFSLE--LYCLVYYFYTISVSLIMLGPELNWLC 536
L+ L+LL + F+ +P + + S R F L L+CL Y +++ LG +L
Sbjct: 523 LLPLFLLVAL-FVVLIMPFHFLYRSTRFFFLTCLLHCLAAPLYKVTLPDFFLGDQLTSQV 581
Query: 535 GRYYDVKIPICIKXTFSILKKKNWC 461
+ IC K++N C
Sbjct: 582 QALRSINFYICYYGWGDFKKRQNTC 606
>At1g20130.1 68414.m02518 family II extracellular lipase, putative
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566)
Length = 1006
Score = 29.9 bits (64), Expect = 4.5
Identities = 15/39 (38%), Positives = 17/39 (43%), Gaps = 4/39 (10%)
Frame = -2
Query: 1475 PPRGGXPXPXPXPXAGXXPK-XGGPXPGPX---XPXPPR 1371
PP+ P P P P G PK P P P P PP+
Sbjct: 101 PPKPPAPTPKPVPPHGPPPKPAPAPTPAPSPKPAPSPPK 139
Score = 29.1 bits (62), Expect = 7.8
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = -2
Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXP 1383
PP G P P P P PK P P P P
Sbjct: 113 PPHGPPPKPAPAPTPAPSPK---PAPSPPKP 140
>At4g32285.1 68417.m04593 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related Aux22d, Vigna radiata, PID:D1021691;
contains Pfam PF01417: ENTH domain. ENTH (Epsin
N-terminal homology) domain; similar to clathrin
assembly protein AP180 (GI:6492344) [Xenopus laevis]
Length = 635
Score = 24.2 bits (50), Expect(2) = 5.1
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = -3
Query: 1474 PPXGGXPPPXPXPXP 1430
PP PPP P P P
Sbjct: 392 PPENHTPPPPPAPEP 406
Score = 23.8 bits (49), Expect(2) = 5.1
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = -3
Query: 1456 PPPXPXPXPGXPQ 1418
PPP P P P PQ
Sbjct: 400 PPPAPEPKPQQPQ 412
>At2g46700.1 68415.m05827 calcium-dependent protein kinase, putative /
CDPK, putative similar to calcium/calmodulin-dependent
protein kinase homolog MCK1 [Zea mays]
gi|1839597|gb|AAB47181
Length = 595
Score = 29.5 bits (63), Expect = 5.9
Identities = 13/35 (37%), Positives = 14/35 (40%), Gaps = 1/35 (2%)
Frame = -2
Query: 1457 PXPXPXPXAGXXPKXGGPXPGPX-XPXPPRFFKXP 1356
P P P P P G P P P RFF+ P
Sbjct: 56 PWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRP 90
>At1g59910.1 68414.m06749 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02128
Length = 929
Score = 29.5 bits (63), Expect = 5.9
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = -2
Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
P P P P P A P P GP P PP
Sbjct: 378 PANQTSPPPPPPPSAAAPPPPPPPKKGPAAPPPP 411
>At5g54650.2 68418.m06805 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02181
Length = 900
Score = 29.1 bits (62), Expect = 7.8
Identities = 17/34 (50%), Positives = 18/34 (52%)
Frame = -2
Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
PPR P P P P +G PK P PGP P PP
Sbjct: 385 PPRP--PPPAPPPGSGG-PKPPPP-PGPKGPRPP 414
>At5g54650.1 68418.m06804 formin homology 2 domain-containing protein
/ FH2 domain-containing protein contains formin homology
2 domain, Pfam:PF02181
Length = 900
Score = 29.1 bits (62), Expect = 7.8
Identities = 17/34 (50%), Positives = 18/34 (52%)
Frame = -2
Query: 1475 PPRGGXPXPXPXPXAGXXPKXGGPXPGPXXPXPP 1374
PPR P P P P +G PK P PGP P PP
Sbjct: 385 PPRP--PPPAPPPGSGG-PKPPPP-PGPKGPRPP 414
>At4g19200.1 68417.m02833 proline-rich family protein contains proline
rich extensin domains, INTERPRO:IPR002965
Length = 179
Score = 29.1 bits (62), Expect = 7.8
Identities = 14/37 (37%), Positives = 16/37 (43%)
Frame = +1
Query: 1366 KNRGGXGXXGPGXGPPXFGXXPAXGXGXGXGXPPRGG 1476
+ +G G G G PP G P G G PP GG
Sbjct: 12 QEKGFHGFPGGGHYPPAQGGYPPQGYPPQQGYPPAGG 48
>At4g08230.1 68417.m01358 glycine-rich protein
Length = 113
Score = 29.1 bits (62), Expect = 7.8
Identities = 14/45 (31%), Positives = 17/45 (37%)
Frame = -2
Query: 1175 LFSXPERDGFSPGRXPGWNWAXXXXXPCXXGGXSXRXGGGVGXSP 1041
+FS + D + G P W GG S GGG G P
Sbjct: 39 MFSMEKSDAYRKGSKPNKKWGGGMGGGGGGGGGSGGGGGGRGGGP 83
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 24.2 bits (50), Expect(2) = 8.4
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = -3
Query: 1474 PPXGGXPPPXPXPXP 1430
PP PPP P P P
Sbjct: 515 PPVYSSPPPPPSPAP 529
Score = 23.0 bits (47), Expect(2) = 8.4
Identities = 14/47 (29%), Positives = 15/47 (31%)
Frame = -3
Query: 1456 PPPXPXPXPGXPQXXGAXXXGLXFXXPPGFLRXPRXXXNPXQXPLPP 1316
PPP P P PQ + PP P P P PP
Sbjct: 537 PPPPPPHSPPPPQFSPPPPEPYYYSSPP-----PPHSSPPPHSPPPP 578
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,550,628
Number of Sequences: 28952
Number of extensions: 478520
Number of successful extensions: 1695
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 979
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1502
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3961461312
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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