BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_A06_e329_02.seq (1516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 28 2.9 SPBC1711.16 |||WD repeat protein Pwp1 |Schizosaccharomyces pombe... 28 2.9 SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 5.1 SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity fac... 27 5.1 SPAC1F7.03 |pkd2||TRP-like ion channel |Schizosaccharomyces pomb... 27 6.8 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 28.3 bits (60), Expect = 2.9 Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 8/57 (14%) Frame = +2 Query: 530 KLKKN--EHMCLLMILYMPSPSISNLSANQ*LQFRLED---LPERTDP---LNPCYT 676 +++KN MCLL I Y P IS+ + + RLED + E+T +NP +T Sbjct: 1429 RIQKNFSNQMCLLSIQYRMHPDISHFPSKKFYDSRLEDGDNMAEKTQQVWHVNPKFT 1485 >SPBC1711.16 |||WD repeat protein Pwp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 516 Score = 28.3 bits (60), Expect = 2.9 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +1 Query: 829 PPSYANEGPYSYDISKPNYTKVPSISQSAKPDS 927 P YA+E P SYDI + Y ++ +S+ D+ Sbjct: 11 PRGYASEFPKSYDIDEIEYERINQLSKLKLEDA 43 >SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1098 Score = 27.5 bits (58), Expect = 5.1 Identities = 11/21 (52%), Positives = 16/21 (76%) Frame = -1 Query: 265 LVCLEEVSNHRPKTIQLIQPL 203 L L +S+H PKT+QL++PL Sbjct: 403 LKSLLSISSHLPKTLQLMEPL 423 >SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity factor complex subunit, Fip1 homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 27.5 bits (58), Expect = 5.1 Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 1/61 (1%) Frame = +1 Query: 736 GFSDKYGV-SNSNYQFSQSGGIYGGEVNYXTKPPSYANEGPYSYDISKPNYTKVPSISQS 912 G+S + V ++SNY + + G GG + P +Y N P S + P+I+ S Sbjct: 218 GYSRNFPVHASSNYNTTHTSG--GGVHSGAATPNAYVNNNPSSSRRESESPANSPNITSS 275 Query: 913 A 915 A Sbjct: 276 A 276 >SPAC1F7.03 |pkd2||TRP-like ion channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 710 Score = 27.1 bits (57), Expect = 6.8 Identities = 10/17 (58%), Positives = 14/17 (82%) Frame = -2 Query: 732 SSISAITYSFWRNFLWS 682 +S+ AI YS+ RNF+WS Sbjct: 223 TSLPAIIYSWGRNFMWS 239 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,656,987 Number of Sequences: 5004 Number of extensions: 83625 Number of successful extensions: 191 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 848370472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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