Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_H09_e168_15.seq
(1513 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 228 2e-60
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 202 1e-52
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 99 8e-22
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 34 0.059
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.96
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 29 1.3
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 28 2.9
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 3.9
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 5.1
SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 27 5.1
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 6.8
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 228 bits (557), Expect = 2e-60
Identities = 114/156 (73%), Positives = 133/156 (85%)
Frame = +2
Query: 236 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 415
P+Y PFFGVMG +AI+F+S GAAYGTAK+G GI+AM V+RP+ I+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 416 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 595
YGLVV+VLI+G+L Q +LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQ
Sbjct: 67 YGLVVSVLISGNLKQI---LSLYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQ 123
Query: 596 PRLFVGMILILIFAEVLGLYGLIVAIYLYTKQ*TNL 703
PRLFV MILILIFAEVLGLYGLIVA+ L T+ N+
Sbjct: 124 PRLFVAMILILIFAEVLGLYGLIVALLLNTRATDNV 159
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 202 bits (492), Expect = 1e-52
Identities = 95/151 (62%), Positives = 120/151 (79%)
Frame = +2
Query: 236 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 415
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE +MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 416 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 595
YGLV++VLIAG + P N+Y+L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q
Sbjct: 67 YGLVMSVLIAGDMS-PDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQ 125
Query: 596 PRLFVGMILILIFAEVLGLYGLIVAIYLYTK 688
R+FV M+LILIFAEVLGLYGLIV + L TK
Sbjct: 126 DRIFVSMVLILIFAEVLGLYGLIVGLILQTK 156
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 99 bits (238), Expect = 8e-22
Identities = 51/147 (34%), Positives = 81/147 (55%), Gaps = 5/147 (3%)
Frame = +2
Query: 254 FGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIYGLVVA 433
+G++G AS + F +GAA+G GT I AV P K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 434 VLIAGSLDQ--PSNNYT---LYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 598
++ + ++ P+ YT Y GF G+ VG L G +GI G + AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 599 RLFVGMILILIFAEVLGLYGLIVAIYL 679
LFV ++++ IF VLGL+GLIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
Score = 41.1 bits (92), Expect = 4e-04
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +2
Query: 500 LGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYL 679
LG V F + A + I I G + + G + PR+ ++ +IF EV+ +Y LI+AI
Sbjct: 48 LGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIAIVF 107
Query: 680 YTK 688
K
Sbjct: 108 SAK 110
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 33.9 bits (74), Expect = 0.059
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = -2
Query: 657 PYRPNTSAKMRIRIIPTNNLGCWAVPRTPASPTMPMAKPAARPEKPTARPAPK*MNP 487
P RP ++A ++ PT +VP P++P MP P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 29.9 bits (64), Expect = 0.96
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = -2
Query: 660 SPYRPNTSAKMRIRIIPTNNLGCWAVPRTPASPTMPMAKPAARPEKPTAR 511
S Y+P++S+K+ + + G A+P+ P P P +P R T +
Sbjct: 401 SSYKPSSSSKVSAKSVSRKPTGAPAIPKLP--PKHPSRQPTVRATPSTGK 448
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 29.5 bits (63), Expect = 1.3
Identities = 21/69 (30%), Positives = 30/69 (43%)
Frame = +2
Query: 380 IIPVVMAGIIAIYGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVGFSGLAAGFAIGI 559
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVI-------AAMVGILYFGHWLVYKILILAFGFKIVT 245
Query: 560 VGDAGVRGT 586
GD V T
Sbjct: 246 SGDVCVSNT 254
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 28.3 bits (60), Expect = 2.9
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -3
Query: 239 LDFRPFFLNVGVFTGPKNSDDYLHTL 162
+DF P V TGP N+DDY+H +
Sbjct: 357 MDF-PNITQVVQITGPSNTDDYIHRI 381
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 3.9
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = -2
Query: 588 AVPRTPAS--PTMPMAKPAARPEKPTARPAP 502
A P P S P++PM PAA P P+A AP
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.5 bits (58), Expect = 5.1
Identities = 16/80 (20%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
Frame = +2
Query: 251 FFG-VMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMR-PEQIMKSIIPVVMAGIIAIYGL 424
F+G ++G + F+ + + ++ G + I+ S+ P+V++ IIA +
Sbjct: 1439 FYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMSVAPLVLSAIIAFFFF 1498
Query: 425 VVAVLIAGSLDQPSNNYTLY 484
+ +++ L S Y +Y
Sbjct: 1499 CLGIMLRPILGDRSKTYGVY 1518
>SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 579
Score = 27.5 bits (58), Expect = 5.1
Identities = 28/90 (31%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
Frame = +2
Query: 422 LVVAVLIAGSLDQPSNNYTLYK--GFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQ 595
L+ + ++ + +N +Y+ G + +A+G +GLA G IG+ G G A
Sbjct: 141 LITSSMLLDTFQTMESNQEMYELSGTRKMRRRIAMGLAGLAGGALIGLTG-----GLAAP 195
Query: 596 PRLFVGMILILIFAEVLGLYGLIVAIYLYT 685
FV L +FA LGL +I A YL T
Sbjct: 196 ---FVAAGLGTLFAG-LGLGTMIGATYLGT 221
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.1 bits (57), Expect = 6.8
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = +2
Query: 239 IYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIY 418
+YGPF+ A+ FS+ Y +G G + ++ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 419 GLVVAVLIAGSL 454
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,065,902
Number of Sequences: 5004
Number of extensions: 104945
Number of successful extensions: 267
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 846388298
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -