Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_H02_e112_16.seq
(1494 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g11890.1 68414.m01371 vesicle transport protein SEC22, putati... 130 2e-30
At5g52270.1 68418.m06487 vesicle transport protein-related simil... 76 7e-14
At5g22360.1 68418.m02609 synaptobrevin family protein similar to... 47 4e-05
At4g32150.1 68417.m04573 synaptobrevin family protein similar to... 43 5e-04
At2g25340.1 68415.m03031 synaptobrevin family protein similar to... 42 0.001
At4g15780.1 68417.m02402 synaptobrevin-related family protein si... 40 0.004
At2g32670.1 68415.m03994 synaptobrevin family protein similar to... 38 0.013
At1g04750.1 68414.m00470 synaptobrevin family protein similar to... 38 0.013
At2g33120.1 68415.m04060 synaptobrevin-related protein / vesicl... 38 0.017
At1g04760.1 68414.m00472 synaptobrevin family protein similar to... 38 0.023
At5g11150.1 68418.m01303 synaptobrevin / vesicle-associated memb... 37 0.030
At3g54300.1 68416.m06001 synaptobrevin family protein similar to... 37 0.039
At2g33110.1 68415.m04059 synaptobrevin family protein similar to... 36 0.069
At5g25590.1 68418.m03045 expressed protein contains Pfam profile... 32 1.1
At3g03750.2 68416.m00381 SET domain-containing protein low simil... 31 2.6
At3g03750.1 68416.m00380 SET domain-containing protein low simil... 31 2.6
At5g58180.1 68418.m07282 SNARE protein-related similar to SNARE ... 30 3.4
>At1g11890.1 68414.m01371 vesicle transport protein SEC22, putative
identified as SEC22 by Raikhel, NV, et al. in Plant
Physiol. 124: 1558-69 (2000); similar to vesicle
trafficking protein gb|U91538 from Mus musculus; ESTs
gb|F15494 and gb|F14097 come from this gene
Length = 218
Score = 130 bits (314), Expect = 2e-30
Identities = 60/124 (48%), Positives = 83/124 (66%), Gaps = 2/124 (1%)
Frame = +3
Query: 216 MVLMTMIARVVDGLPLAATMQEDEQSGCNVLEYQNQAKMLFRKL--GPQSPMRCSIETGP 389
MV MT+IARV DGLPLA + + + + Y+ Q K LF+ L G R S+ETGP
Sbjct: 1 MVKMTLIARVTDGLPLAEGLDDGRDLPDSDM-YKQQVKALFKNLSRGQNDASRMSVETGP 59
Query: 390 YLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFYQQYGKRVNTVTRPYTFIEFDTW 569
Y+FHY+IE +CYL +C+R+Y K+LAF YLE++ EF + G + T RPY FI+FDT+
Sbjct: 60 YVFHYIIEGRVCYLTMCDRSYPKKLAFQYLEDLKNEFERVNGPNIETAARPYAFIKFDTF 119
Query: 570 MQRT 581
+Q+T
Sbjct: 120 IQKT 123
>At5g52270.1 68418.m06487 vesicle transport protein-related similar
to vesicle trafficking protein sec22b [Mus musculus]
GI:1907386
Length = 214
Score = 75.8 bits (178), Expect = 7e-14
Identities = 38/119 (31%), Positives = 72/119 (60%), Gaps = 4/119 (3%)
Frame = +3
Query: 216 MVLMTMIARVVDGLPLAATMQE-DEQSGCNVLEYQNQAKMLFRKLGPQSPM--RCSIETG 386
MV +T++ RV DGLPLA +++ + L Y+ QA+ L +++ S + + +I
Sbjct: 1 MVKLTIVGRVEDGLPLAQDQTYVNQEDNTSFLLYKQQAEFLLKQVSKDSLLHPKMTILLD 60
Query: 387 PYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFYQQYGKR-VNTVTRPYTFIEF 560
+ FH+L+E +ICY+ L + +Y ++L F+YL+ + +E + K + +++PY+FI F
Sbjct: 61 HHSFHFLVEKKICYIALSDSSYPRKLLFNYLQNLNKELDKLDEKALIQKISKPYSFIRF 119
>At5g22360.1 68418.m02609 synaptobrevin family protein similar to
Synaptobrevin-like protein 1 (SP:P51809) [Homo sapiens]
Length = 221
Score = 46.8 bits (106), Expect = 4e-05
Identities = 22/65 (33%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Frame = +3
Query: 327 KMLFRKLGPQ-SPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
+ + KL P+ S R Y+FH L + + +L + + +R+ FSYLEEI F
Sbjct: 30 RRILEKLSPEISDERLCFSQDRYIFHILRSDGLTFLCMANDTFGRRVPFSYLEEIHMRFM 89
Query: 504 QQYGK 518
+ YGK
Sbjct: 90 KNYGK 94
Score = 29.1 bits (62), Expect = 7.9
Identities = 15/61 (24%), Positives = 32/61 (52%), Gaps = 6/61 (9%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKD------ATYLNTKSMLVKAT 819
+++ +M++NI+ +++RG + L KT + S ++K A ++ +LV T
Sbjct: 137 EIRSVMVENIEKIMERGDRIELLVDKTATMQDSSFHFRKQSKRLRRALWMKNAKLLVLLT 196
Query: 820 C 822
C
Sbjct: 197 C 197
>At4g32150.1 68417.m04573 synaptobrevin family protein similar to
Synaptobrevin-like protein 1 (SP:P51809) [Homo sapiens]
Length = 219
Score = 43.2 bits (97), Expect = 5e-04
Identities = 20/69 (28%), Positives = 35/69 (50%)
Frame = +3
Query: 324 AKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
AK + K+ + S Y+FH + + L + E +R+ F++LE+I Q F
Sbjct: 29 AKQILEKVPGDNDSNVSYSQDRYVFHVKRTDGLTVLCMAEETAGRRIPFAFLEDIHQRFV 88
Query: 504 QQYGKRVNT 530
+ YG+ V+T
Sbjct: 89 RTYGRAVHT 97
>At2g25340.1 68415.m03031 synaptobrevin family protein similar to
Synaptobrevin-like protein 1 (SP:P51809) [Homo sapiens]
Length = 219
Score = 41.5 bits (93), Expect = 0.001
Identities = 18/69 (26%), Positives = 35/69 (50%)
Frame = +3
Query: 324 AKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
AK + K+ S Y+FH + + L + + + +R+ FS+LE+I Q F
Sbjct: 29 AKQILEKIPGNGDSHVSYSQDRYVFHVKRTDGLTVLCMADEDAGRRIPFSFLEDIHQRFV 88
Query: 504 QQYGKRVNT 530
+ YG+ +++
Sbjct: 89 RTYGRAIHS 97
>At4g15780.1 68417.m02402 synaptobrevin-related family protein
similar to Vesicle-associated membrane protein 722
(AtVAMP722) Synaptobrevin-related protein 1 (SP:P47192)
{Arabidopsis thaliana}
Length = 194
Score = 39.9 bits (89), Expect = 0.004
Identities = 19/54 (35%), Positives = 32/54 (59%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNTKSMLVKAT 819
+V+ IM++NID + RG L+ L KT+NL +++YKK + + +V T
Sbjct: 141 EVKSIMLENIDKAIDRGENLTVLTDKTENLRSQAREYKKTKGHRWRRKFVVTRT 194
Score = 38.7 bits (86), Expect = 0.010
Identities = 16/64 (25%), Positives = 36/64 (56%)
Frame = +3
Query: 324 AKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
A +KL S + + + F++L+E+ Y V+ + + SK+++ ++LE + +F
Sbjct: 32 AAQCLQKLPSSSNSKFTYNCDHHTFNFLVEDAYAYCVVAKDSLSKQISIAFLERVKADFK 91
Query: 504 QQYG 515
++YG
Sbjct: 92 KRYG 95
>At2g32670.1 68415.m03994 synaptobrevin family protein similar to
vesicle-associated membrane protein 7 [Rattus
norvegicus] GI:9502258, SP|P51809 Synaptobrevin-like
protein 1 {Homo sapiens}; contains Pfam profile PF00957:
Synaptobrevin
Length = 285
Score = 38.3 bits (85), Expect = 0.013
Identities = 17/64 (26%), Positives = 35/64 (54%)
Frame = +3
Query: 324 AKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
A +KL P S + + + F+YL+EN Y V+ + +++ ++LE + ++F
Sbjct: 97 AAQCLQKL-PSSNNKFTYNCDGHTFNYLVENGFTYCVVAVESVGRQIPMAFLERVKEDFN 155
Query: 504 QQYG 515
++YG
Sbjct: 156 KRYG 159
Score = 37.5 bits (83), Expect = 0.023
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNTK 798
+V+ +MM+NI+ VL RG + L KT+NL +Q ++ T + K
Sbjct: 205 EVKGVMMENIEKVLDRGEKIELLVDKTENLRSQAQDFRTQGTKIRRK 251
>At1g04750.1 68414.m00470 synaptobrevin family protein similar to
Vesicle-associated membrane protein 722 (AtVAMP722)
Synaptobrevin-related protein 1 (SP:P47192) {Arabidopsis
thaliana};
Length = 219
Score = 38.3 bits (85), Expect = 0.013
Identities = 18/50 (36%), Positives = 29/50 (58%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNTKSML 807
+V+ +MM+NI+ VL RG + L KT+NL +Q ++ T + K L
Sbjct: 140 EVKGVMMENIEKVLDRGEKIELLVDKTENLRSQAQDFRTTGTQMRRKMWL 189
Score = 36.3 bits (80), Expect = 0.052
Identities = 17/64 (26%), Positives = 35/64 (54%)
Frame = +3
Query: 324 AKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
A +KL P S + + + F+YL+E+ Y V+ + +++ S+LE + ++F
Sbjct: 32 AAQCLQKL-PSSNNKFTYNCDGHTFNYLVEDGFTYCVVAVDSAGRQIPMSFLERVKEDFN 90
Query: 504 QQYG 515
++YG
Sbjct: 91 KRYG 94
>At2g33120.1 68415.m04060 synaptobrevin-related protein /
vesicle-associated membrane protein 722 (VAMP722) (SAR1)
identical to r to Vesicle-associated membrane protein
722 (AtVAMP722) Synaptobrevin-related protein 1
(SP:P47192) {Arabidopsis thaliana}; identical to cDNA
synaptobrevin-related protein (SAR1) GI:600709
Length = 221
Score = 37.9 bits (84), Expect = 0.017
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNTK 798
+V+ +MM+NI+ VL RG + L KT+NL +Q ++ T + K
Sbjct: 140 EVKGVMMENIEKVLDRGEKIELLVDKTENLRSQAQDFRTQGTQMRRK 186
Score = 37.1 bits (82), Expect = 0.030
Identities = 17/64 (26%), Positives = 35/64 (54%)
Frame = +3
Query: 324 AKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFY 503
A +KL P S + + + F+YL+EN Y V+ + +++ ++LE + ++F
Sbjct: 32 AAQCLQKL-PSSNNKFTYNCDGHTFNYLVENGFTYCVVAVDSAGRQIPMAFLERVKEDFN 90
Query: 504 QQYG 515
++YG
Sbjct: 91 KRYG 94
>At1g04760.1 68414.m00472 synaptobrevin family protein similar to
Vesicle-associated membrane protein 722 (AtVAMP722)
Synaptobrevin-related protein 1 (SP:P47192) {Arabidopsis
thaliana}
Length = 220
Score = 37.5 bits (83), Expect = 0.023
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNTK 798
+V+ +MM+NI+ VL RG + L KT+NL +Q ++ T + K
Sbjct: 140 EVKGVMMENIEKVLDRGEKIELLVDKTENLRSQAQDFRTQGTKMKRK 186
Score = 34.7 bits (76), Expect = 0.16
Identities = 16/68 (23%), Positives = 36/68 (52%)
Frame = +3
Query: 312 YQNQAKMLFRKLGPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIA 491
+ + A +KL P S + + + F+YL +N Y V+ + +++ ++LE +
Sbjct: 28 FTSVAAQCLQKL-PSSNNKFTYNCDGHTFNYLADNGFTYCVVVIESAGRQIPMAFLERVK 86
Query: 492 QEFYQQYG 515
++F ++YG
Sbjct: 87 EDFNKRYG 94
>At5g11150.1 68418.m01303 synaptobrevin / vesicle-associated
membrane protein 713 (VAMP713) identified as AtVAMP713
by Sanderfoot, A.A., et al. in Plant Physiol. 124:
1558-69 (2000); similar to Vesicle-associated membrane
protein 722 (AtVAMP722) Synaptobrevin-related protein 1
(SP:P47192) {Arabidopsis thaliana}; synaptobrevin-like
protein Sybl1, Mus musculus, EMBL:MMU133536
Length = 221
Score = 37.1 bits (82), Expect = 0.030
Identities = 17/70 (24%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
Frame = +3
Query: 324 AKMLFRKL-GPQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEF 500
+K + KL G S S Y+FH + + L + + + + F++L++I Q F
Sbjct: 29 SKQILEKLPGNDSDSHMSYSQDRYIFHVKRTDGLTVLCMADETAGRNIPFAFLDDIHQRF 88
Query: 501 YQQYGKRVNT 530
+ YG+ +++
Sbjct: 89 VKTYGRAIHS 98
Score = 35.1 bits (77), Expect = 0.12
Identities = 17/45 (37%), Positives = 27/45 (60%)
Frame = +1
Query: 661 VQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNT 795
V+ +M++NID VL RG L L KT+N+ + +++K A T
Sbjct: 138 VRNVMIENIDKVLDRGERLELLVDKTENMQGNTFRFRKQARRYRT 182
>At3g54300.1 68416.m06001 synaptobrevin family protein similar to
vesicle-associated membrane protein 7B (At VAMP7B),
Arabidopsis thaliana, EMBL:AF025333
Length = 240
Score = 36.7 bits (81), Expect = 0.039
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = +1
Query: 658 DVQRIMMQNIDDVLQRGAILSELDTKTQNLSMMSQKYKKDATYLNTKSML 807
+V+ IMM NI+ VL RG + L KT+NL + +++ L K L
Sbjct: 159 EVKGIMMDNIEKVLDRGEKIELLVDKTENLQFQADSFQRQGRQLRRKMWL 208
>At2g33110.1 68415.m04059 synaptobrevin family protein similar to
Vesicle-associated membrane protein 722 (AtVAMP722)
Synaptobrevin-related protein 1 (SP:P47192) {Arabidopsis
thaliana}
Length = 217
Score = 35.9 bits (79), Expect = 0.069
Identities = 14/55 (25%), Positives = 30/55 (54%)
Frame = +3
Query: 351 PQSPMRCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFYQQYG 515
P S + + + F+ L+EN Y V+ + + + ++LE + ++FY++YG
Sbjct: 40 PSSNNKFTYNCDGHTFNDLVENGFTYCVVAVDSAGREIPMAFLERVKEDFYKRYG 94
>At5g25590.1 68418.m03045 expressed protein contains Pfam profile
PF04783: Protein of unknown function (DUF630)
Length = 775
Score = 31.9 bits (69), Expect = 1.1
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = -2
Query: 344 FSKQHFSLILILQNITAALLVFLHGRSQRQAVDD 243
F+ HF+ + L+N AAL + HG S ++A+DD
Sbjct: 34 FAAGHFAYAIALKNTGAALSDYGHGESDQKALDD 67
>At3g03750.2 68416.m00381 SET domain-containing protein low
similarity to G9a [Homo sapiens] GI:287865; contains
Pfam profiles PF00856: SET domain, PF05033: Pre-SET
motif
Length = 354
Score = 30.7 bits (66), Expect = 2.6
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +3
Query: 405 LIENEICYLVLCERNYSKRLAFSYLEEIAQE 497
L++ C CE Y K LAF+ +EEIA E
Sbjct: 139 LVDESGCECERCEEGYCKCLAFAGMEEIANE 169
>At3g03750.1 68416.m00380 SET domain-containing protein low
similarity to G9a [Homo sapiens] GI:287865; contains
Pfam profiles PF00856: SET domain, PF05033: Pre-SET
motif
Length = 338
Score = 30.7 bits (66), Expect = 2.6
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +3
Query: 405 LIENEICYLVLCERNYSKRLAFSYLEEIAQE 497
L++ C CE Y K LAF+ +EEIA E
Sbjct: 139 LVDESGCECERCEEGYCKCLAFAGMEEIANE 169
>At5g58180.1 68418.m07282 SNARE protein-related similar to SNARE
protein Ykt6 [Homo sapiens] GI:2507637
Length = 199
Score = 30.3 bits (65), Expect = 3.4
Identities = 16/66 (24%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
Frame = +3
Query: 366 RCSIETGPYLFHYLIENEICYLVLCERNYSKRLAFSYLEEIAQEFYQQYGK--RVNTVTR 539
R S++ Y H N +C + + +Y R AFS L ++ + + YG R ++
Sbjct: 60 RQSVKHEEYKVHAYNINGLCAVGFMDDHYPVRSAFSLLNQVLDVYQKDYGDTWRFENSSQ 119
Query: 540 PYTFIE 557
P+ +++
Sbjct: 120 PWPYLK 125
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,038,202
Number of Sequences: 28952
Number of extensions: 347601
Number of successful extensions: 748
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 746
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3980738496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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