Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_G04_e127_14.seq
(1506 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 38 0.013
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 38 0.013
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 37 0.030
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 32 1.1
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 30 4.6
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 30 4.6
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 29 6.0
At3g02060.1 68416.m00169 DEAD/DEAH box helicase, putative simila... 29 8.0
At1g79580.3 68414.m09279 no apical meristem (NAM) family protein... 29 8.0
At1g79580.2 68414.m09278 no apical meristem (NAM) family protein... 29 8.0
At1g79580.1 68414.m09277 no apical meristem (NAM) family protein... 29 8.0
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 38.3 bits (85), Expect = 0.013
Identities = 17/65 (26%), Positives = 35/65 (53%)
Frame = -1
Query: 678 LDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKPLLHCIQVAKAKAIVYS 499
L +++GDV+ + + N E+V++++G + + AVS N + L ++ + AK I+
Sbjct: 95 LGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITH 154
Query: 498 DHLAD 484
D
Sbjct: 155 SQYVD 159
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 38.3 bits (85), Expect = 0.013
Identities = 17/65 (26%), Positives = 35/65 (53%)
Frame = -1
Query: 678 LDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKPLLHCIQVAKAKAIVYS 499
L +++GDV+ + + N E+V++++G + + AVS N + L ++ + AK I+
Sbjct: 95 LGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITH 154
Query: 498 DHLAD 484
D
Sbjct: 155 SQYVD 159
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 37.1 bits (82), Expect = 0.030
Identities = 20/65 (30%), Positives = 32/65 (49%)
Frame = -1
Query: 678 LDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKPLLHCIQVAKAKAIVYS 499
L ++ GDVV + +PN E+ ++L +A L AVS N + + + AK I+
Sbjct: 93 LGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASAAKMIITK 152
Query: 498 DHLAD 484
L D
Sbjct: 153 KCLVD 157
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 31.9 bits (69), Expect = 1.1
Identities = 18/66 (27%), Positives = 31/66 (46%)
Frame = -1
Query: 681 HLDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKPLLHCIQVAKAKAIVY 502
+L +K+ DVV + +PN E V T+L + + A++ N + + + AK IV
Sbjct: 82 NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141
Query: 501 SDHLAD 484
D
Sbjct: 142 QSRYVD 147
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 29.9 bits (64), Expect = 4.6
Identities = 18/76 (23%), Positives = 33/76 (43%)
Frame = -1
Query: 711 SNQVARVMQQHLDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKPLLHCI 532
S Q+A + L + + DVV + +PNC E+V ++L + A + N +
Sbjct: 75 SRQIAANFHK-LGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQA 133
Query: 531 QVAKAKAIVYSDHLAD 484
+ + K I+ D
Sbjct: 134 KASNTKLIITEARYVD 149
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 29.9 bits (64), Expect = 4.6
Identities = 18/76 (23%), Positives = 33/76 (43%)
Frame = -1
Query: 711 SNQVARVMQQHLDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKPLLHCI 532
S Q+A + L + + DVV + +PNC E+V ++L + A + N +
Sbjct: 75 SRQIAANFHK-LGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQA 133
Query: 531 QVAKAKAIVYSDHLAD 484
+ + K I+ D
Sbjct: 134 KASNTKLIITEARYVD 149
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 29.5 bits (63), Expect = 6.0
Identities = 21/79 (26%), Positives = 35/79 (44%), Gaps = 1/79 (1%)
Frame = -1
Query: 726 QMASKSNQVARVMQQHLDLKRGDVVCVFMPNCGEYVWTWLGMAKLSAVSALINSNLRHKP 547
Q + ++A + Q L + GDVV V PN V G+ A+ L N+RH
Sbjct: 46 QTRDRCVRIASALSQ-LGISTGDVVSVLAPNVPAMVELHFGVPMAGAL--LCTLNIRHDS 102
Query: 546 LLHCIQVA-KAKAIVYSDH 493
L + + ++++DH
Sbjct: 103 SLVAVLLRHSGTKVIFADH 121
>At3g02060.1 68416.m00169 DEAD/DEAH box helicase, putative similar
to C-terminal half of transcription-repair coupling
factor (TRCF) GB:Q55750 [Synechocystis PCC6803];
contains Pfam profile: helicases conserved C-terminal
domain
Length = 823
Score = 29.1 bits (62), Expect = 8.0
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +1
Query: 187 PLKTHISDYQSARVIYSIKLRVDMNKRIY 273
P+KTH+S ++ +VI +IK +D +++
Sbjct: 456 PIKTHLSSFRKEKVIEAIKNELDRGGQVF 484
>At1g79580.3 68414.m09279 no apical meristem (NAM) family protein
similar to OsNAC7 protein (GI:6730944) [Oryza sativa];
contains weak hit to Pfam PF02365 : No apical meristem
(NAM) protein
Length = 371
Score = 29.1 bits (62), Expect = 8.0
Identities = 16/36 (44%), Positives = 17/36 (47%)
Frame = +1
Query: 583 DSTELGHSEPRPYILSTVGHKHAHHITSLQIEMLLH 690
DS HS P IL + H H HH QI M LH
Sbjct: 201 DSNSNNHS---PLILHPLDHHHHHHHIGRQIHMPLH 233
>At1g79580.2 68414.m09278 no apical meristem (NAM) family protein
similar to OsNAC7 protein (GI:6730944) [Oryza sativa];
contains weak hit to Pfam PF02365 : No apical meristem
(NAM) protein
Length = 371
Score = 29.1 bits (62), Expect = 8.0
Identities = 16/36 (44%), Positives = 17/36 (47%)
Frame = +1
Query: 583 DSTELGHSEPRPYILSTVGHKHAHHITSLQIEMLLH 690
DS HS P IL + H H HH QI M LH
Sbjct: 201 DSNSNNHS---PLILHPLDHHHHHHHIGRQIHMPLH 233
>At1g79580.1 68414.m09277 no apical meristem (NAM) family protein
similar to OsNAC7 protein (GI:6730944) [Oryza sativa];
contains weak hit to Pfam PF02365 : No apical meristem
(NAM) protein
Length = 371
Score = 29.1 bits (62), Expect = 8.0
Identities = 16/36 (44%), Positives = 17/36 (47%)
Frame = +1
Query: 583 DSTELGHSEPRPYILSTVGHKHAHHITSLQIEMLLH 690
DS HS P IL + H H HH QI M LH
Sbjct: 201 DSNSNNHS---PLILHPLDHHHHHHHIGRQIHMPLH 233
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,505,868
Number of Sequences: 28952
Number of extensions: 465579
Number of successful extensions: 915
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 885
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 915
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4019292864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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