Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_G01_e103_13.seq
         (1451 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc...   143   5e-35
SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom...   143   5e-35
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   8.6  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    27   8.6  

>SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein
           L20|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score =  143 bits (347), Expect = 5e-35
 Identities = 75/139 (53%), Positives = 90/139 (64%), Gaps = 4/139 (2%)
 Frame = +1

Query: 82  SRFWYFLRQLKKFKKTTGEIVSIKEIPEKSPVKIKNFGIWLRYESRSGVHNMYREYRDLS 261
           SR+WYFL+ + K KK TGEIV+I EI E  P+K K FGIW+RY+SRSG HNMY+E+RD +
Sbjct: 38  SRYWYFLKMINKVKKATGEIVAINEISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTT 97

Query: 262 VGGAVTQCYRDMGARHRARAHSIQIIK-VEVIKAAACRRPQVKQFHTSKIGFPLPKR--- 429
             GAV   Y DM ARHRAR  SI+I+K VEV K    RR  VKQ     + FPLP R   
Sbjct: 98  RVGAVEAMYADMAARHRARFRSIRILKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTG 157

Query: 430 VHQYKRLNTFAYKRPSTYF 486
           V        FA  RPST++
Sbjct: 158 VVGLAGKKVFAPHRPSTFY 176


>SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal
           protein L20a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score =  143 bits (347), Expect = 5e-35
 Identities = 75/139 (53%), Positives = 90/139 (64%), Gaps = 4/139 (2%)
 Frame = +1

Query: 82  SRFWYFLRQLKKFKKTTGEIVSIKEIPEKSPVKIKNFGIWLRYESRSGVHNMYREYRDLS 261
           SR+WYFL+ + K KK TGEIV+I EI E  P+K K FGIW+RY+SRSG HNMY+E+RD +
Sbjct: 38  SRYWYFLKMINKVKKATGEIVAINEISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTT 97

Query: 262 VGGAVTQCYRDMGARHRARAHSIQIIK-VEVIKAAACRRPQVKQFHTSKIGFPLPKR--- 429
             GAV   Y DM ARHRAR  SI+I+K VEV K    RR  VKQ     + FPLP R   
Sbjct: 98  RVGAVEAMYADMAARHRARFRSIRILKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTG 157

Query: 430 VHQYKRLNTFAYKRPSTYF 486
           V        FA  RPST++
Sbjct: 158 VVGLAGKKVFAPHRPSTFY 176


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 26.6 bits (56), Expect = 8.6
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +2

Query: 572 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 736
           P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231

Query: 737 SCA 745
           S A
Sbjct: 232 SDA 234


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 26.6 bits (56), Expect = 8.6
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +1

Query: 679 PPFASWRNSEEARTDRPSQQLRSLN 753
           PP AS RN  E  +  PS+Q  S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,072,436
Number of Sequences: 5004
Number of extensions: 98184
Number of successful extensions: 226
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 810768624
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)