Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_F08_e158_12.seq
         (1524 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...   101   2e-22
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    36   0.019
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    30   0.97 
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   1.3  
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces...    28   3.9  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    28   3.9  
SPAC664.03 |||RNA polymerase II associated Paf1 complex |Schizos...    27   5.2  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    27   5.2  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   6.8  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score =  101 bits (243), Expect = 2e-22
 Identities = 56/140 (40%), Positives = 82/140 (58%)
 Frame = +1

Query: 145 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTG 324
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + ++D+FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60

Query: 325 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 504
           +CRY ++DFE+ +  +G      + K+  +SW PD A +K KM+YSSS D L+++  G+ 
Sbjct: 61  DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114

Query: 505 KYIQATDLSEASQEAVEEKL 564
             IQATD SE + E V EK+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 35.5 bits (78), Expect = 0.019
 Identities = 27/86 (31%), Positives = 44/86 (51%)
 Frame = +1

Query: 211 RYVVFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 390
           R  +  I +E   DV+T+  ++   +S   D +K    EC  G  +  +        + S
Sbjct: 26  RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76

Query: 391 KKQKLFLMSWCPDTAKVKKKMLYSSS 468
           KK  L L+S+ P+ A V++KMLY+SS
Sbjct: 77  KKNLLQLISYVPENANVRRKMLYASS 102



 Score = 28.7 bits (61), Expect = 2.2
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +1

Query: 400 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 552
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 29.9 bits (64), Expect = 0.97
 Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
 Frame = +1

Query: 619 PHTHATNDACAAPCH-IPWFETAAPQYTPTGGAPQLTLKQTPLLGSLPTGRRPPVHSSTG 795
           P   A   + AAP +  PW + AAPQ      AP L++  +    SLP  ++P   S+  
Sbjct: 423 PAPWAKPASSAAPSNPAPWQQPAAPQ-----SAPALSMNPS----SLPPWQQPTQQSAVQ 473

Query: 796 WCSLFTQQTFPFIGG 840
             +L   Q  PFI G
Sbjct: 474 PSNLVPSQNAPFIPG 488


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 376 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 555
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 556 EKL 564
           E L
Sbjct: 138 EFL 140


>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 446

 Score = 27.9 bits (59), Expect = 3.9
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = -1

Query: 465 ARVQHLLLDLGGVWAPRH*EQFLFLARLRCTLALMRVFKIEE 340
           A ++ LLL +     PR  ++ +   R+ CT  L  VF++EE
Sbjct: 93  ASLEELLLKIFN--GPRSDKELILTIRIMCTFCLASVFQVEE 132


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 27.9 bits (59), Expect = 3.9
 Identities = 16/54 (29%), Positives = 25/54 (46%)
 Frame = -2

Query: 419 HDIRNNFCFLLASDVPWH*CVYSKSKRPYLHSPVPPFCRSSRKLSYSALRPPTV 258
           HD+ ++  +++ S   +H C  ++S  P  HS  PP            LRPP V
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFP--HSHHPPLHNPLPVSCQPVLRPPPV 158


>SPAC664.03 |||RNA polymerase II associated Paf1 complex
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 457

 Score = 27.5 bits (58), Expect = 5.2
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +3

Query: 699 SNGRRPSAHAQTDAAPRFTSNGPAPP 776
           +NG +P   AQ  +A    +N PAPP
Sbjct: 413 TNGVKPETQAQNMSASESQANSPAPP 438


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 27.5 bits (58), Expect = 5.2
 Identities = 27/136 (19%), Positives = 61/136 (44%), Gaps = 1/136 (0%)
 Frame = +1

Query: 172 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTGECRYGLFDF 351
           K + + ++ + K ++     +D K+ D + +  +  EY SF ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 352 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLS 531
           +   +  G    S+K    ++      A V K      ++   +K++    K ++  +  
Sbjct: 167 Q--KKVHGPPALSEKPGKCILK-DKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEFE 223

Query: 532 EASQ-EAVEEKLRATD 576
             ++ E  EEK + ++
Sbjct: 224 SINEGETEEEKAKTSN 239


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +1

Query: 466 SFDALKKSLVGVQKYIQATDLSEASQ 543
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,357,026
Number of Sequences: 5004
Number of extensions: 83377
Number of successful extensions: 271
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 254
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 265
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 854316994
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)