BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_F08_e158_12.seq
(1524 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_55463| Best HMM Match : OPA3 (HMM E-Value=0) 62 2e-09
SB_30343| Best HMM Match : No HMM Matches (HMM E-Value=.) 59 1e-08
SB_28028| Best HMM Match : Cofilin_ADF (HMM E-Value=2e-20) 56 1e-07
SB_35589| Best HMM Match : No HMM Matches (HMM E-Value=.) 53 5e-07
SB_14227| Best HMM Match : Cofilin_ADF (HMM E-Value=0.0013) 47 5e-05
SB_8589| Best HMM Match : DUF164 (HMM E-Value=0.29) 34 0.34
SB_3044| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.34
SB_49450| Best HMM Match : SH3_1 (HMM E-Value=6.9e-17) 32 1.4
SB_38286| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.4
SB_36002| Best HMM Match : Peptidase_A17 (HMM E-Value=9.3e-41) 31 3.2
SB_20964| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 3.2
SB_5400| Best HMM Match : DUF1153 (HMM E-Value=7.8) 30 4.2
SB_50916| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 5.6
SB_13311| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 5.6
SB_44895| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 9.8
SB_45423| Best HMM Match : RRM_1 (HMM E-Value=0) 29 9.8
SB_30225| Best HMM Match : Peptidase_S8 (HMM E-Value=0.0034) 29 9.8
>SB_55463| Best HMM Match : OPA3 (HMM E-Value=0)
Length = 387
Score = 61.7 bits (143), Expect = 2e-09
Identities = 41/146 (28%), Positives = 75/146 (51%), Gaps = 3/146 (2%)
Frame = +1
Query: 139 MASGVTVSDACKTTYEEIKKDKKH-RYVVFYIRDEKQIDVETVGGRNAE--YDSFLEDLQ 309
M +G+ + + EIKK RY++F + ++K+ V E ++ L+DL
Sbjct: 247 MEAGLNIKGEVTDGWNEIKKAASGLRYIIFKMDEKKENVVFEKKKMKCECSHEDVLDDLP 306
Query: 310 KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKS 489
E RY + +Y + E + + KL L+ WCPD ++K +M+ +++F +KK
Sbjct: 307 ---ADEPRYIALNLDYKNV-----EGADRSKLVLIFWCPDNCEIKSRMVSAATFQDVKKK 358
Query: 490 LVGVQKYIQATDLSEASQEAVEEKLR 567
G K ++ + SE S EA++E+L+
Sbjct: 359 CPGGAKCLEIQERSELSFEALKEELK 384
>SB_30343| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 140
Score = 58.8 bits (136), Expect = 1e-08
Identities = 41/144 (28%), Positives = 72/144 (50%), Gaps = 3/144 (2%)
Frame = +1
Query: 145 SGVTVSDACKTTYEEIKKDKKH-RYVVFYIRDEKQIDVETVGGRNAE--YDSFLEDLQKG 315
+G+ + + EIK RY +F + ++K+ V E +D L+DL
Sbjct: 2 AGLNIKSDVTDGWNEIKMVASGLRYTIFKMDEKKENVVMEKKKMITECCHDDVLDDLP-- 59
Query: 316 GTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLV 495
T E RY + +Y ++ E +++ KL L+ WCPD +K KM+ +++F + K
Sbjct: 60 -TDEPRYVALNLDYKNE-----EGAERSKLVLIFWCPDNCGIKNKMVSAATFKEVMKKCP 113
Query: 496 GVQKYIQATDLSEASQEAVEEKLR 567
G K ++ D + S EA++EKL+
Sbjct: 114 GGAKCLEIQDRFDLSFEALKEKLK 137
>SB_28028| Best HMM Match : Cofilin_ADF (HMM E-Value=2e-20)
Length = 151
Score = 55.6 bits (128), Expect = 1e-07
Identities = 40/150 (26%), Positives = 66/150 (44%), Gaps = 7/150 (4%)
Frame = +1
Query: 145 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV-----ETVGGRNAEYDS--FLED 303
SG+ V D + +K K H+Y +F I DE + V ++V E D F +
Sbjct: 4 SGIKVDDESLKLSQTMKSMKTHKYAIFKICDEANMVVIDQTFKSVVTNTREEDRAIFYQM 63
Query: 304 LQKGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALK 483
++K E RY L+D ++ + E L +SWC D A ++KKM +S+ D ++
Sbjct: 64 VEKLSDREPRYILYDMKFPRK----EEKRIFNNLVFISWCSDKAPIEKKMKLASTQDYVR 119
Query: 484 KSLVGVQKYIQATDLSEASQEAVEEKLRAT 573
K + A D + + + K T
Sbjct: 120 KKFSEATTSVNANDFDDLDYDEIAAKAEKT 149
>SB_35589| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 150
Score = 53.2 bits (122), Expect = 5e-07
Identities = 39/150 (26%), Positives = 70/150 (46%), Gaps = 11/150 (7%)
Frame = +1
Query: 145 SGVTVSDACKTTYEEIK-KDKKHRYVVFYIRDE----------KQIDVETVGGRNAEYDS 291
SG+ + D Y+ ++ K+K H++ F I D+ K++D T A +D
Sbjct: 4 SGIKIDDESLHLYQTMQGKEKSHKFATFKISDDGKMVVIDHILKRVDTHTREEDRAIFDQ 63
Query: 292 FLEDLQKGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSF 471
LE L E RY L+D + + + L + WC D A +KK+M+ +++
Sbjct: 64 MLEKLSDS---EPRYILYDLNFPRK-----DGRAFHHLVYIFWCSDNAPIKKRMVSAATN 115
Query: 472 DALKKSLVGVQKYIQATDLSEASQEAVEEK 561
+ LK V V+K Q D ++ + + + +K
Sbjct: 116 ELLKTKFV-VKKVFQINDRADLNYDDIADK 144
>SB_14227| Best HMM Match : Cofilin_ADF (HMM E-Value=0.0013)
Length = 310
Score = 46.8 bits (106), Expect = 5e-05
Identities = 22/58 (37%), Positives = 33/58 (56%)
Frame = +1
Query: 361 HQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSE 534
H+ Q A FL C D A +KKKML S+++ LKK G++KY +A+++ E
Sbjct: 241 HKTQDHRSALSDLVFFLFDRCSDEAPIKKKMLAGSTWEYLKKKFDGLKKYFEASEICE 298
>SB_8589| Best HMM Match : DUF164 (HMM E-Value=0.29)
Length = 306
Score = 33.9 bits (74), Expect = 0.34
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +3
Query: 687 PPIHSNGRRPSAHAQTDAAPRFTSNGP 767
P +H NG RPSAH +T P TSN P
Sbjct: 2 PQVHMNGGRPSAHRKTSKQPS-TSNSP 27
>SB_3044| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1170
Score = 33.9 bits (74), Expect = 0.34
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +3
Query: 687 PPIHSNGRRPSAHAQTDAAPRFTSNGP 767
P +H NG RPSAH +T P TSN P
Sbjct: 2 PQVHMNGGRPSAHRKTSKQPS-TSNSP 27
>SB_49450| Best HMM Match : SH3_1 (HMM E-Value=6.9e-17)
Length = 288
Score = 31.9 bits (69), Expect = 1.4
Identities = 22/46 (47%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 581 SESDAF-SSPLNSPLTHTLRTTRARPLVTYRGSRPPPPNTLQRAAP 715
++S +F SS + SPLT L RPLVT S PP T Q AAP
Sbjct: 20 TQSPSFTSSVVTSPLTVNL----CRPLVTIATSCPPVTTTAQAAAP 61
>SB_38286| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 288
Score = 31.9 bits (69), Expect = 1.4
Identities = 22/46 (47%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 581 SESDAF-SSPLNSPLTHTLRTTRARPLVTYRGSRPPPPNTLQRAAP 715
++S +F SS + SPLT L RPLVT S PP T Q AAP
Sbjct: 20 TQSPSFTSSVVTSPLTVNL----CRPLVTIATSCPPVTTTAQAAAP 61
>SB_36002| Best HMM Match : Peptidase_A17 (HMM E-Value=9.3e-41)
Length = 193
Score = 30.7 bits (66), Expect = 3.2
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = +1
Query: 250 DVETVGGRNAEYDSFLEDLQKGGT---GECRYGLFDFEYTHQCQGTSEASKK 396
D E G R + S+L+DL++ C YG+ E T+ G +ASKK
Sbjct: 83 DEELTGERKKRWLSWLDDLKRVKEVRIARCVYGVSGAEVTYSLHGFGDASKK 134
>SB_20964| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 311
Score = 30.7 bits (66), Expect = 3.2
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = +1
Query: 250 DVETVGGRNAEYDSFLEDLQKGGT---GECRYGLFDFEYTHQCQGTSEASKK 396
D E G R + S+L+DL++ C YG+ E T+ G +ASKK
Sbjct: 139 DEELTGERKKRWLSWLDDLKRVKEVRIARCVYGVSGAEVTYSLHGFGDASKK 190
>SB_5400| Best HMM Match : DUF1153 (HMM E-Value=7.8)
Length = 170
Score = 30.3 bits (65), Expect = 4.2
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = +1
Query: 688 PQYTPTGGAPQLTLKQTPLLGSLPTGRRPPVH 783
P TP+ PQ + +TPLL S PT P H
Sbjct: 100 PSKTPSSVPPQAKVDETPLLPSPPTSTPRPSH 131
>SB_50916| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1681
Score = 29.9 bits (64), Expect = 5.6
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = +1
Query: 250 DVETVGGRNAEYDSFLEDLQKGGT---GECRYGLFDFEYTHQCQGTSEASKK 396
D E G R + S+L+DL++ C YG + E T+ G +ASKK
Sbjct: 422 DEELTGERKKRWLSWLDDLKRVKEVRIARCVYGGSEAEVTYSLHGFGDASKK 473
>SB_13311| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 6406
Score = 29.9 bits (64), Expect = 5.6
Identities = 23/79 (29%), Positives = 34/79 (43%)
Frame = +3
Query: 489 SRRRTEVHTGDRPLGGVAGGRRGETPRH*PSVNPMHLALPSTPPSHTRYERRVRGPLSHT 668
++RRT T P RRG P +P P+T + R ++ GP + T
Sbjct: 5903 AKRRTNGSTTRTPEVKATPSRRGGAVSRIPRSSPA--GSPATSFTRERSRDKLSGPAAAT 5960
Query: 669 VVRDRRPPIHSNGRRPSAH 725
+ R+R P +G SAH
Sbjct: 5961 LTRERSPSFTRSG---SAH 5976
>SB_44895| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 237
Score = 29.1 bits (62), Expect = 9.8
Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 3/34 (8%)
Frame = -2
Query: 356 YSKSKRPYLHSPVP-PFCRS--SRKLSYSALRPP 264
YS RPY HSPV P+C S R YS + P
Sbjct: 112 YSPVDRPYCHSPVDRPYCHSPVDRPYCYSPVDHP 145
>SB_45423| Best HMM Match : RRM_1 (HMM E-Value=0)
Length = 514
Score = 29.1 bits (62), Expect = 9.8
Identities = 18/55 (32%), Positives = 22/55 (40%), Gaps = 1/55 (1%)
Frame = +3
Query: 612 TPPSHTRYERRVRGP-LSHTVVRDRRPPIHSNGRRPSAHAQTDAAPRFTSNGPAP 773
TPP H RR R P H+ + R PP H R D R + P+P
Sbjct: 70 TPPRH---RRRSRSPNKKHSRSKSRTPPRHRRRSRSPRRESIDRKRRSSYESPSP 121
>SB_30225| Best HMM Match : Peptidase_S8 (HMM E-Value=0.0034)
Length = 605
Score = 29.1 bits (62), Expect = 9.8
Identities = 18/60 (30%), Positives = 24/60 (40%)
Frame = +3
Query: 606 PSTPPSHTRYERRVRGPLSHTVVRDRRPPIHSNGRRPSAHAQTDAAPRFTSNGPAPPSSL 785
P P + Y R + P T PP SN + P+ + Q A N P PP +L
Sbjct: 355 PVPPSPNLGYSRTEKVPGGPTYTFGPPPPAASNAK-PAQYTQYTAPAPVVYNPPVPPKAL 413
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 34,665,431
Number of Sequences: 59808
Number of extensions: 705264
Number of successful extensions: 2113
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2086
length of database: 16,821,457
effective HSP length: 85
effective length of database: 11,737,777
effective search space used: 4953341894
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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