Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_F07_e150_11.seq
(1535 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q7QHQ3 Cluster: ENSANGP00000008238; n=2; Culicidae|Rep:... 105 3e-21
UniRef50_Q8MQJ7 Cluster: LD18893p; n=4; Drosophila|Rep: LD18893p... 103 1e-20
UniRef50_UPI0000D56DEC Cluster: PREDICTED: similar to CG10967-PA... 99 3e-19
UniRef50_Q23023 Cluster: Serine/threonine-protein kinase unc-51;... 81 8e-14
UniRef50_Q8IYT8 Cluster: Serine/threonine-protein kinase ULK2; n... 52 3e-05
UniRef50_Q6C7U0 Cluster: Serine/threonine-protein kinase ATG1; n... 49 3e-04
UniRef50_UPI0000E45E10 Cluster: PREDICTED: similar to UNC-51-lik... 46 0.002
UniRef50_UPI0000F1ECF4 Cluster: PREDICTED: hypothetical protein;... 43 0.025
UniRef50_O75385 Cluster: Serine/threonine-protein kinase ULK1; n... 42 0.033
UniRef50_Q6BS08 Cluster: Serine/threonine-protein kinase ATG1; n... 37 1.6
UniRef50_P53104 Cluster: Serine/threonine-protein kinase ATG1; n... 36 2.1
>UniRef50_Q7QHQ3 Cluster: ENSANGP00000008238; n=2; Culicidae|Rep:
ENSANGP00000008238 - Anopheles gambiae str. PEST
Length = 740
Score = 105 bits (252), Expect = 3e-21
Identities = 53/115 (46%), Positives = 79/115 (68%), Gaps = 6/115 (5%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEAGV------TPATCDKILYEHAIELCQMAA 313
L+P+ V+NV++ M+ KY+ + ES++L+ AG+ + T DKI++E A+++CQMAA
Sbjct: 616 LKPSNSVKNVLTTMHAKYRSTLIESKKLNSAGLLQRANASNITADKIIFEFALQMCQMAA 675
Query: 314 IEELFGDMKECERRYMSAQVLLHSLVQRHPMHPQHRTTLSKYRDAVQKRLNCLKG 478
++ELF EC RY SAQ+LLH L Q+ HPQ + LS Y++AV+KRL LKG
Sbjct: 676 VDELFNKPAECFPRYQSAQILLHWLAQK-SKHPQDKILLSNYKEAVEKRLYILKG 729
>UniRef50_Q8MQJ7 Cluster: LD18893p; n=4; Drosophila|Rep: LD18893p -
Drosophila melanogaster (Fruit fly)
Length = 855
Score = 103 bits (247), Expect = 1e-20
Identities = 52/114 (45%), Positives = 79/114 (69%), Gaps = 6/114 (5%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEAGVTPA------TCDKILYEHAIELCQMAA 313
L+P+ V+N + MN KY+ ++ ES+RL+ +G+ T DKILY++A+++CQ AA
Sbjct: 731 LKPSSNVKNALLTMNAKYRSMLFESKRLNGSGLLQKANAFNITADKILYDYALDMCQAAA 790
Query: 314 IEELFGDMKECERRYMSAQVLLHSLVQRHPMHPQHRTTLSKYRDAVQKRLNCLK 475
++EL + K C RY +A +LLHSLVQ+ HPQ + L+KYRDAV+KRL+ L+
Sbjct: 791 LDELLKNTKNCFERYNTAHILLHSLVQK-CNHPQDKMMLNKYRDAVEKRLSILQ 843
>UniRef50_UPI0000D56DEC Cluster: PREDICTED: similar to CG10967-PA;
n=3; Endopterygota|Rep: PREDICTED: similar to CG10967-PA
- Tribolium castaneum
Length = 779
Score = 99.1 bits (236), Expect = 3e-19
Identities = 48/112 (42%), Positives = 79/112 (70%), Gaps = 4/112 (3%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHE----AGVTPATCDKILYEHAIELCQMAAIE 319
L+P+ +V+NV+S +N +++ + E ++L+ A V T DK+LY+HA+++CQ AA++
Sbjct: 656 LRPSSKVKNVMSSLNSRFRSTLAECKQLNSPELMAKVADITADKLLYDHAVQICQAAALD 715
Query: 320 ELFGDMKECERRYMSAQVLLHSLVQRHPMHPQHRTTLSKYRDAVQKRLNCLK 475
ELF + ++C RY +AQ+LLHSL Q+ P Q R L+KY++AV+KRL L+
Sbjct: 716 ELFDNPQQCFERYQTAQILLHSLSQQLPQ--QDRALLTKYKEAVEKRLFMLQ 765
>UniRef50_Q23023 Cluster: Serine/threonine-protein kinase unc-51; n=2;
Caenorhabditis|Rep: Serine/threonine-protein kinase
unc-51 - Caenorhabditis elegans
Length = 856
Score = 81.0 bits (191), Expect = 8e-14
Identities = 44/115 (38%), Positives = 73/115 (63%), Gaps = 7/115 (6%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEAGVT---PA----TCDKILYEHAIELCQMA 310
L P+ V+ V++ +N KY + S+ L G+ PA + ++I+Y HAIELCQ A
Sbjct: 733 LHPSVAVQQVLNQLNDKYHQCLVRSQELASLGLPGQDPAMAVISAERIMYRHAIELCQAA 792
Query: 311 AIEELFGDMKECERRYMSAQVLLHSLVQRHPMHPQHRTTLSKYRDAVQKRLNCLK 475
A++ELFG+ + C +RY +A ++LH+L ++ Q +T L++Y+ AV+KRL L+
Sbjct: 793 ALDELFGNPQLCSQRYQTAYMMLHTLAEQVNC-DQDKTVLTRYKVAVEKRLRILE 846
>UniRef50_Q8IYT8 Cluster: Serine/threonine-protein kinase ULK2; n=17;
Euteleostomi|Rep: Serine/threonine-protein kinase ULK2 -
Homo sapiens (Human)
Length = 1036
Score = 52.4 bits (120), Expect = 3e-05
Identities = 33/120 (27%), Positives = 60/120 (50%), Gaps = 13/120 (10%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEA-------------GVTPATCDKILYEHAI 292
L P+ V+ VV +N +YK+ + ++L E + T +K++Y A+
Sbjct: 911 LSPSTAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAV 970
Query: 293 ELCQMAAIEELFGDMKECERRYMSAQVLLHSLVQRHPMHPQHRTTLSKYRDAVQKRLNCL 472
E+ Q AA++E+F ++ RY A +LL L R P + KY+ ++++RL+ L
Sbjct: 971 EMVQSAALDEMFQQTEDIVYRYHKAALLLEGL-SRILQDPADIENVHKYKCSIERRLSAL 1029
>UniRef50_Q6C7U0 Cluster: Serine/threonine-protein kinase ATG1; n=2;
cellular organisms|Rep: Serine/threonine-protein kinase
ATG1 - Yarrowia lipolytica (Candida lipolytica)
Length = 710
Score = 49.2 bits (112), Expect = 3e-04
Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
Frame = +2
Query: 242 AGVTPATCDKILYEHAIELCQMAAIEELFGDMKECERRYMSAQVLLHSLVQ--RHPMHPQ 415
+G T T +K++++ A+E+ + AA++E+ GD CE Y ++ +L +L++ + +
Sbjct: 627 SGTTHTTAEKLIFDRALEMSRDAAVQEISGDFTGCESAYTTSIWMLEALLEDDEDGLGEE 686
Query: 416 HRTTLSKYRDAVQKRLNCLKGXRK 487
R + ++ ++ KRL L+G ++
Sbjct: 687 DRRIVERFISSITKRLVILRGQQE 710
>UniRef50_UPI0000E45E10 Cluster: PREDICTED: similar to UNC-51-like
kinase ULK1; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to UNC-51-like kinase ULK1 -
Strongylocentrotus purpuratus
Length = 750
Score = 46.4 bits (105), Expect = 0.002
Identities = 27/81 (33%), Positives = 44/81 (54%), Gaps = 10/81 (12%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEAGVTPA----------TCDKILYEHAIELC 301
L+P+ VR V+ +N Y + +SR+L E + T DK++Y +AIE C
Sbjct: 670 LKPSNAVRTVLQELNRVYHLCLIKSRQLCEGSPLQSLDIDLNSAMITADKLMYSYAIEQC 729
Query: 302 QMAAIEELFGDMKECERRYMS 364
Q A ++E+FG+ +E + Y S
Sbjct: 730 QSAGMDEMFGNTQEVSKEYDS 750
>UniRef50_UPI0000F1ECF4 Cluster: PREDICTED: hypothetical protein; n=1;
Danio rerio|Rep: PREDICTED: hypothetical protein - Danio
rerio
Length = 927
Score = 42.7 bits (96), Expect = 0.025
Identities = 32/121 (26%), Positives = 55/121 (45%), Gaps = 13/121 (10%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEA-------------GVTPATCDKILYEHAI 292
L P+ V+ VV +N YK VN R L E + T ++++Y H I
Sbjct: 803 LYPSGSVKQVVRELNEMYKECVNSCRSLTEKLQHFFSSKQRLMDRMNSITAERLIYAHTI 862
Query: 293 ELCQMAAIEELFGDMKECERRYMSAQVLLHSLVQRHPMHPQHRTTLSKYRDAVQKRLNCL 472
++ Q AA++E+F + RY A +L+ L P ++K + +++RL L
Sbjct: 863 QMVQTAALDEMFHHGESSLERYHKALLLMEGL-SLIITEPSDLNNVNKCKRCIERRLCSL 921
Query: 473 K 475
+
Sbjct: 922 Q 922
>UniRef50_O75385 Cluster: Serine/threonine-protein kinase ULK1; n=32;
Eumetazoa|Rep: Serine/threonine-protein kinase ULK1 -
Homo sapiens (Human)
Length = 1050
Score = 42.3 bits (95), Expect = 0.033
Identities = 21/72 (29%), Positives = 43/72 (59%), Gaps = 1/72 (1%)
Frame = +2
Query: 260 TCDKILYEHAIELCQMAAIEELFGDMKECERRYMSAQVLLHSLVQRHPMHPQ-HRTTLSK 436
T +++++ HA+++ Q AA++E+F + C RY A +LL L +H + Q ++K
Sbjct: 975 TAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGL--QHMLSDQADIENVTK 1032
Query: 437 YRDAVQKRLNCL 472
+ +++RL+ L
Sbjct: 1033 CKLCIERRLSAL 1044
>UniRef50_Q6BS08 Cluster: Serine/threonine-protein kinase ATG1; n=2;
Saccharomycetaceae|Rep: Serine/threonine-protein kinase
ATG1 - Debaryomyces hansenii (Yeast) (Torulaspora
hansenii)
Length = 875
Score = 36.7 bits (81), Expect = 1.6
Identities = 23/90 (25%), Positives = 46/90 (51%), Gaps = 1/90 (1%)
Frame = +2
Query: 242 AGVTPATCDKILYEHAIELCQMAAIEELFG-DMKECERRYMSAQVLLHSLVQRHPMHPQH 418
A T +K++++ A+E+ + AA+ EL D+K CE Y +A +L +L+ + +
Sbjct: 785 ADKTRVVAEKLIFDRALEMSRNAAVNELVKEDLKGCELAYSTAIWMLEALLDEDSSNDED 844
Query: 419 RTTLSKYRDAVQKRLNCLKGXRKIMDVKLE 508
R + + V+K + + ++ KLE
Sbjct: 845 RLD-DEDKAMVEKFIVSIGNRLSVLKRKLE 873
>UniRef50_P53104 Cluster: Serine/threonine-protein kinase ATG1; n=2;
Saccharomyces cerevisiae|Rep: Serine/threonine-protein
kinase ATG1 - Saccharomyces cerevisiae (Baker's yeast)
Length = 897
Score = 36.3 bits (80), Expect = 2.1
Identities = 24/81 (29%), Positives = 45/81 (55%), Gaps = 3/81 (3%)
Frame = +2
Query: 152 LQPTPQVRNVVSLMNGKYKWIVNESRRLHEAGVT--PATCDKILYEHAIELCQMAAIEEL 325
L+ +R ++ + K+ V E++ L E G + P +K+LY+ A+E+ +MAA EL
Sbjct: 750 LEKADFLRLKINDLRFKHASEVAENQTLEEKGSSEEPVYLEKLLYDRALEISKMAAHMEL 809
Query: 326 FGD-MKECERRYMSAQVLLHS 385
G+ + CE Y ++ +L +
Sbjct: 810 KGENLYNCELAYATSLWMLET 830
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 687,169,020
Number of Sequences: 1657284
Number of extensions: 9662787
Number of successful extensions: 21080
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 20526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21064
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 164134746325
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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