Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_E01_e101_09.seq
(1348 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_46525| Best HMM Match : Thymosin (HMM E-Value=0) 98 1e-20
SB_11031| Best HMM Match : VPS9 (HMM E-Value=8.3e-21) 33 0.40
SB_52732| Best HMM Match : M (HMM E-Value=0.019) 33 0.69
SB_16625| Best HMM Match : Death (HMM E-Value=1.5) 32 1.2
SB_19898| Best HMM Match : Merozoite_SPAM (HMM E-Value=3.7) 31 2.8
SB_50941| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 8.5
SB_48646| Best HMM Match : Sulfotransfer_1 (HMM E-Value=8.5) 29 8.5
SB_34166| Best HMM Match : RVT_1 (HMM E-Value=1e-23) 29 8.5
SB_12216| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 8.5
SB_37693| Best HMM Match : PHD (HMM E-Value=8.7e-35) 29 8.5
>SB_46525| Best HMM Match : Thymosin (HMM E-Value=0)
Length = 750
Score = 98.3 bits (234), Expect = 1e-20
Identities = 55/148 (37%), Positives = 83/148 (56%), Gaps = 2/148 (1%)
Frame = +2
Query: 131 KSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDATSLKHTETQEKNP 310
+++++ F S L+ V+T EK LP+ + +A EK + + F G+E F LKH ET EKNP
Sbjct: 451 RAEVKSFEKSKLQHVETKEKNTLPTKDTIADEK-RTAPFSGVEVFQKNKLKHVETLEKNP 509
Query: 311 LPDKDVVAAEKAHQNLLE--GVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXXXNKFLNGI 484
LPD + AE + L + V FD +++KH T+EK + ++ +
Sbjct: 510 LPDAQNIRAEMMPEVLPDRSEVAKFDTSKLKHVETKEKVVMPTKDVIEAEAIDSR--AEV 567
Query: 485 ESFDPTKLKHTETCEKNPLPTKDVIEQE 568
+SFD +KLKH T EKNPLPT + +E
Sbjct: 568 KSFDHSKLKHVVTQEKNPLPTPQTLHEE 595
Score = 98.3 bits (234), Expect = 1e-20
Identities = 57/159 (35%), Positives = 85/159 (53%), Gaps = 2/159 (1%)
Frame = +2
Query: 107 LPKVANDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDATSLKH 286
+PK D +S++ F+ + L+ V T EK ++PS ED+ E ++ FD + LKH
Sbjct: 597 IPKNKPD-RSEVASFDHTKLKHVTTQEKSIMPSQEDIKEEAVDSRA--EVKSFDHSKLKH 653
Query: 287 TETQEKNPLPDKDVVAAEKAHQNL--LEGVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXX 460
ET EKNPLP V+ E + L + V FD +++KH +EKN L
Sbjct: 654 VETVEKNPLPSAAVLKEEMRPEVLPDVSAVASFDASKLKHVEVQEKNPLPTKDDITTEST 713
Query: 461 XNKFLNGIESFDPTKLKHTETCEKNPLPTKDVIEQEKSA 577
+ +++FD +KLKH +T EKNPLP I QEK++
Sbjct: 714 ETRA--EVKTFDHSKLKHVQTEEKNPLPDAKTIAQEKAS 750
Score = 89.8 bits (213), Expect = 4e-18
Identities = 58/156 (37%), Positives = 85/156 (54%), Gaps = 4/156 (2%)
Frame = +2
Query: 119 ANDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIE--KFDATSLKHTE 292
A D +++++ F+ S L+ V T EK LP+ + + E K+ D E FD T LKH
Sbjct: 560 AIDSRAEVKSFDHSKLKHVVTQEKNPLPTPQTLHEELIPKNKPDRSEVASFDHTKLKHVT 619
Query: 293 TQEKNPLPDKDVVAAEKAHQNLLEGVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXXXNKF 472
TQEK+ +P ++ + E+A + E V+ FD +++KH T EKN L
Sbjct: 620 TQEKSIMPSQEDIK-EEAVDSRAE-VKSFDHSKLKHVETVEKNPLPSAAVLKEEMRPEVL 677
Query: 473 --LNGIESFDPTKLKHTETCEKNPLPTKDVIEQEKS 574
++ + SFD +KLKH E EKNPLPTKD I E +
Sbjct: 678 PDVSAVASFDASKLKHVEVQEKNPLPTKDDITTEST 713
Score = 81.4 bits (192), Expect = 2e-15
Identities = 47/133 (35%), Positives = 68/133 (51%), Gaps = 2/133 (1%)
Frame = +2
Query: 176 DTNEKIVLPSAEDVATEKTQKSLFDGIEKFDATSLKHTETQEKNPLPDKDVVAAEKAHQN 355
DT ++I P +E + K + KFDA +LKH +T+EKN LP + + E
Sbjct: 389 DTPQQI-WPGSEVAYVDGGSKPDVSEVAKFDAANLKHVQTKEKNTLPSDETIKQELQPDE 447
Query: 356 LLE--GVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXXXNKFLNGIESFDPTKLKHTETCE 529
+ V+ F+K++++H T+EKN+L F +G+E F KLKH ET E
Sbjct: 448 FPDRAEVKSFEKSKLQHVETKEKNTLPTKDTIADEKRTAPF-SGVEVFQKNKLKHVETLE 506
Query: 530 KNPLPTKDVIEQE 568
KNPLP I E
Sbjct: 507 KNPLPDAQNIRAE 519
Score = 57.2 bits (132), Expect = 3e-08
Identities = 32/88 (36%), Positives = 45/88 (51%), Gaps = 2/88 (2%)
Frame = +2
Query: 314 PDKDVVAAEKAHQNLLEGVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXXXNKFLNGIE-- 487
P +V + + + V FD +KH T+EKN+L ++F + E
Sbjct: 396 PGSEVAYVDGGSKPDVSEVAKFDAANLKHVQTKEKNTLPSDETIKQELQPDEFPDRAEVK 455
Query: 488 SFDPTKLKHTETCEKNPLPTKDVIEQEK 571
SF+ +KL+H ET EKN LPTKD I EK
Sbjct: 456 SFEKSKLQHVETKEKNTLPTKDTIADEK 483
>SB_11031| Best HMM Match : VPS9 (HMM E-Value=8.3e-21)
Length = 900
Score = 33.5 bits (73), Expect = 0.40
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 1/77 (1%)
Frame = +2
Query: 170 DVDTNEKIVLPSAEDVATEKTQKSLFDG-IEKFDATSLKHTETQEKNPLPDKDVVAAEKA 346
DV + P+ A EKT K + DG +EK+ + +T + L D DV E+
Sbjct: 341 DVIGKPPAIPPAQVQAAEEKTPKDIIDGLLEKYKEIGSQMADTSADDGLDDDDV---EEE 397
Query: 347 HQNLLEGVEHFDKTQMK 397
++L+ + F T+ K
Sbjct: 398 DDDVLDEADRFRDTKRK 414
>SB_52732| Best HMM Match : M (HMM E-Value=0.019)
Length = 1366
Score = 32.7 bits (71), Expect = 0.69
Identities = 21/75 (28%), Positives = 30/75 (40%)
Frame = +2
Query: 98 LKDLPKVANDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDATS 277
L DL +V +LKS+ EG CL D++ + DV E I + +
Sbjct: 1125 LMDLSRVGEELKSENEGLQQKCL-DLEKQRDTIKQDLADVQKEHENLKSAVSISEGELLK 1183
Query: 278 LKHTETQEKNPLPDK 322
K+T EK K
Sbjct: 1184 FKNTLQSEKEEFEKK 1198
>SB_16625| Best HMM Match : Death (HMM E-Value=1.5)
Length = 528
Score = 31.9 bits (69), Expect = 1.2
Identities = 33/150 (22%), Positives = 57/150 (38%), Gaps = 24/150 (16%)
Frame = +2
Query: 200 PSAEDV--ATEKTQKSLFDGIEKFDATSLKHTE-----------TQEKNPLPDKDVVAAE 340
PS+E A T L DG++ F +H + T E++P DK A
Sbjct: 169 PSSEKSYQALRDTTHDLLDGLQHFGQAKEEHAKLIPKYHEIMIKTIEQDPTSDKSYKALR 228
Query: 341 KAHQNLLEGVEHFDKTQMKHTT-----------TEEKNSLXXXXXXXXXXXXNKFLNGIE 487
+LL+G+ + D+ + +H T E++ + L+G+
Sbjct: 229 DTTHDLLDGLRYSDQAKEEHAKLIPKYHEIMIKTIEQDPTSDKSYKALRDTTHDLLDGLR 288
Query: 488 SFDPTKLKHTETCEKNPLPTKDVIEQEKSA 577
FD K +H + K IEQ+ ++
Sbjct: 289 DFDQAKEEHAKLIPKYHKIMIKTIEQDPTS 318
>SB_19898| Best HMM Match : Merozoite_SPAM (HMM E-Value=3.7)
Length = 446
Score = 30.7 bits (66), Expect = 2.8
Identities = 19/59 (32%), Positives = 27/59 (45%)
Frame = +2
Query: 236 KSLFDGIEKFDATSLKHTETQEKNPLPDKDVVAAEKAHQNLLEGVEHFDKTQMKHTTTE 412
KS+F + S+ TET+E NP D AE+ ++ EG T+ K T E
Sbjct: 276 KSVFKRNRPTEPASVPSTETKEANPNVTADTKQAEQNEGSMSEGTTSKTITENKTTNME 334
>SB_50941| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 316
Score = 29.1 bits (62), Expect = 8.5
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 823 SR*KLCKKRFYFLQTK*YGDASILKLLNGRGYNAVFKK 710
+R ++ KR F+++ YG +S LL G+ YN KK
Sbjct: 162 NRKEVSGKRTVFIESGIYGSSSTAALLQGKSYNRGVKK 199
>SB_48646| Best HMM Match : Sulfotransfer_1 (HMM E-Value=8.5)
Length = 674
Score = 29.1 bits (62), Expect = 8.5
Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 5/86 (5%)
Frame = +2
Query: 170 DVDTNEKIVLPSAEDVATEK-----TQKSLFDGIEKFDATSLKHTETQEKNPLPDKDVVA 334
D+ ++VLP+ +DV K + GI + D+ S+ + + +PD ++
Sbjct: 341 DLAQQLQVVLPNVKDVTFTPFCWKFAYKFEYTGITESDS-SMSLARVRRTSSVPDSLQLS 399
Query: 335 AEKAHQNLLEGVEHFDKTQMKHTTTE 412
AE +E V+H +K + TT +
Sbjct: 400 AELLSVGSVESVDHREKYPLNKTTAD 425
>SB_34166| Best HMM Match : RVT_1 (HMM E-Value=1e-23)
Length = 385
Score = 29.1 bits (62), Expect = 8.5
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = +2
Query: 197 LPSAEDVATEKTQKSLFDGIEKFDATSLKHTETQEKNPLP 316
L S D EKT D +E+F + + +H T +KNPLP
Sbjct: 194 LSSRSDHVQEKT-----DRLEQFSSQTGRHINTTKKNPLP 228
>SB_12216| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2992
Score = 29.1 bits (62), Expect = 8.5
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 2/70 (2%)
Frame = +3
Query: 552 MSSSKRNPLESLPRISLTLATTISR*LSPYFKLDVSPLHIIGPFALSILNVSFFL--NTA 725
M S NPLE L R + +T R YF + + P ++ P + L +F++ N
Sbjct: 2835 MVKSAANPLEILNRRKVKRSTKQRRLFDVYFSICLVPDTLVEPRDVRYLRPAFYVVFNFW 2894
Query: 726 L*PLPFRSFR 755
L LP +R
Sbjct: 2895 LAELPLEKYR 2904
>SB_37693| Best HMM Match : PHD (HMM E-Value=8.7e-35)
Length = 2049
Score = 29.1 bits (62), Expect = 8.5
Identities = 13/52 (25%), Positives = 28/52 (53%)
Frame = +2
Query: 206 AEDVATEKTQKSLFDGIEKFDATSLKHTETQEKNPLPDKDVVAAEKAHQNLL 361
++D EKT+ S+ + + D+++ T+EK +KD E+ ++L+
Sbjct: 1546 SDDGTDEKTEDSMQEDAKDLDSSNTASDTTKEKGKNKEKDKTVLERWEESLM 1597
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 28,799,985
Number of Sequences: 59808
Number of extensions: 552571
Number of successful extensions: 1401
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1363
length of database: 16,821,457
effective HSP length: 84
effective length of database: 11,797,585
effective search space used: 4294320940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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