Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_E01_e101_09.seq
         (1348 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containi...    31   1.3  
At2g39710.1 68415.m04872 aspartyl protease family protein contai...    31   1.7  
At5g22640.1 68418.m02645 MORN (Membrane Occupation and Recogniti...    30   3.0  
At3g20410.1 68416.m02585 calmodulin-domain protein kinase isofor...    30   3.0  
At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger) fa...    29   5.3  
At3g11240.1 68416.m01367 arginine-tRNA-protein transferase, puta...    29   7.0  
At1g50700.1 68414.m05701 calcium-dependent protein kinase, putat...    29   9.2  
At1g29470.1 68414.m03605 dehydration-responsive protein-related ...    29   9.2  

>At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containing
           protein contains Pfam profile PF01388: ARID/BRIGHT DNA
           binding domain
          Length = 786

 Score = 31.5 bits (68), Expect = 1.3
 Identities = 21/68 (30%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
 Frame = +2

Query: 47  IFIPD*TMACSVGDAPSLKDLPKVANDLKSQLEGF---NTSCLRDVDTNEKIVLPSAEDV 217
           I I + T   S+ +  +  DLPK+ ++  SQ E     + S    +DT E ++ P+AED 
Sbjct: 68  IVIEEGTSLASLEEVTNADDLPKIDDEKNSQFETSPHPSPSPSVALDTEEGLINPTAEDT 127

Query: 218 ATEKTQKS 241
             E    S
Sbjct: 128 VEENIVSS 135


>At2g39710.1 68415.m04872 aspartyl protease family protein contains 
           profile Pfam PF00026: Eukaryotic aspartyl protease;
           contains Prosite PS00141: Eukaryotic and viral aspartyl
           proteases active site.;
          Length = 442

 Score = 31.1 bits (67), Expect = 1.7
 Identities = 20/41 (48%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = -1

Query: 595 IRGSDSSGFLLLDDI-FSWEWILFASFSVLQLCRVEAFDAV 476
           I GSDSSGFLLL D  +SW   +  +  VLQ   +  FD V
Sbjct: 217 ISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLPYFDRV 257


>At5g22640.1 68418.m02645 MORN (Membrane Occupation and Recognition
           Nexus) repeat-containing protein contains Pfam profile
           PF02493: MORN repeat
          Length = 871

 Score = 30.3 bits (65), Expect = 3.0
 Identities = 20/67 (29%), Positives = 33/67 (49%)
 Frame = +2

Query: 224 EKTQKSLFDGIEKFDATSLKHTETQEKNPLPDKDVVAAEKAHQNLLEGVEHFDKTQMKHT 403
           EK+ K + DG+EK+     K  E +++    + ++V AE     L E +E  D+   K  
Sbjct: 581 EKSVKPMLDGLEKWTEEKKKAYEERKEMIQQELELVEAEIC---LEEAIEDMDEELKKKE 637

Query: 404 TTEEKNS 424
             EEK +
Sbjct: 638 QEEEKKT 644


>At3g20410.1 68416.m02585 calmodulin-domain protein kinase isoform 9
           (CPK9) identical to calmodulin-domain protein kinase
           CDPK isoform 9 [Arabidopsis thaliana]
           gi|1399265|gb|AAB03242
          Length = 541

 Score = 30.3 bits (65), Expect = 3.0
 Identities = 13/62 (20%), Positives = 29/62 (46%)
 Frame = +2

Query: 341 KAHQNLLEGVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXXXNKFLNGIESFDPTKLKHTE 520
           ++++NL +  +HFDK    + T +E  S              + L+ ++S +  ++ + E
Sbjct: 464 ESNENLYKAFQHFDKDSSGYITIDELESALKEYGMGDDATIKEVLSDVDSDNDGRINYEE 523

Query: 521 TC 526
            C
Sbjct: 524 FC 525


>At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger)
           family protein contains similarity to polycomb-M33
           interacting protein Ring1B [Mus musculus] GI:2239142;
           contains Pfam profile PF00097: Zinc finger, C3HC4 type
           (RING finger)
          Length = 486

 Score = 29.5 bits (63), Expect = 5.3
 Identities = 22/86 (25%), Positives = 37/86 (43%)
 Frame = +1

Query: 289 RNPGEEPSA*QRCCRSGKGTSELTGRRRALRQNANEAHDDGREEFFTAN*SYRSRKREK* 468
           R+ G+E +   R  R   G+      R   +QNA+EAH+D   +    + + R R ++  
Sbjct: 242 RSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDND----DNNNRGRDKDSS 297

Query: 469 VPERHRKLRPDKAEAH*NLRKESTPN 546
             ER  ++R  K       R    P+
Sbjct: 298 SDERGTEVRQKKRRKRSTSRSTQHPS 323


>At3g11240.1 68416.m01367 arginine-tRNA-protein transferase,
           putative / arginyltransferase, putative /
           arginyl-tRNA-protein transferase, putative similar to
           SP|Q9ZT48 Arginine-tRNA-protein transferase 1 (EC
           2.3.2.8) (R-transferase 1) (Arginyltransferase 1)
           (Arginyl-tRNA--protein transferase 1) {Arabidopsis
           thaliana}; contains Pfam profiles PF04377:
           Arginine-tRNA-protein transferase C terminus, PF04376:
           Arginine-tRNA-protein transferase N terminus
          Length = 605

 Score = 29.1 bits (62), Expect = 7.0
 Identities = 24/100 (24%), Positives = 46/100 (46%), Gaps = 11/100 (11%)
 Frame = +2

Query: 104 DLPKVANDLKSQLEGFNTSCLRDVDTNEKIVLPSA--EDVATEKTQKSLFDGIEKFDATS 277
           +L  +  DL  Q++     C++  +    + +P A  + V + K +K L +G E    TS
Sbjct: 155 ELEPIMKDLSEQIDNAVQKCIQSGEFPSNVQIPKASVKKVFSAK-RKKLAEGSEDLLYTS 213

Query: 278 ---------LKHTETQEKNPLPDKDVVAAEKAHQNLLEGV 370
                    +KHT+T EK    +++ ++ E   + LL  +
Sbjct: 214 NIAFPIVAAMKHTQTLEKGKNVEENRLSPEAVSEKLLSAM 253


>At1g50700.1 68414.m05701 calcium-dependent protein kinase, putative
           / CDPK, putative similar to calmodulin-domain protein
           kinase CDPK isoform 9 [Arabidopsis thaliana]
           gi|1399265|gb|AAB03242
          Length = 521

 Score = 28.7 bits (61), Expect = 9.2
 Identities = 11/62 (17%), Positives = 30/62 (48%)
 Frame = +2

Query: 341 KAHQNLLEGVEHFDKTQMKHTTTEEKNSLXXXXXXXXXXXXNKFLNGIESFDPTKLKHTE 520
           ++++N+ +  +HFDK    + TT+E  +              + L+ +++ +  ++ + E
Sbjct: 446 ESNENVYKAFQHFDKDGSGYITTDELEAALKEYGMGDDATIKEILSDVDADNDGRINYDE 505

Query: 521 TC 526
            C
Sbjct: 506 FC 507


>At1g29470.1 68414.m03605 dehydration-responsive protein-related
           similar to early-responsive to dehydration stress ERD3
           protein [Arabidopsis thaliana] GI:15320410; contains
           Pfam profile PF03141: Putative methyltransferase
          Length = 770

 Score = 28.7 bits (61), Expect = 9.2
 Identities = 22/107 (20%), Positives = 46/107 (42%)
 Frame = +2

Query: 101 KDLPKVANDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDATSL 280
           KD+    +D KS+     +    D    E  V+    +  T+  +    +  EK ++   
Sbjct: 57  KDVDTTKSDFKSEEVDRGSKSFPDEKNEETEVVTETNEEKTDPEKSGEENSGEKTESAE- 115

Query: 281 KHTETQEKNPLPDKDVVAAEKAHQNLLEGVEHFDKTQMKHTTTEEKN 421
           +  E  +KN   D+     EK  ++  +  +  +KTQ++ ++ E K+
Sbjct: 116 ERKEFDDKNGDGDRKNGDGEKDTESESDETKQKEKTQLEESSEENKS 162


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,393,154
Number of Sequences: 28952
Number of extensions: 384585
Number of successful extensions: 1008
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 970
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1008
length of database: 12,070,560
effective HSP length: 83
effective length of database: 9,667,544
effective search space used: 3528653560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)