BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030712_D10_e172_08.seq (1510 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 248 2e-66 SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|... 245 1e-65 SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom... 208 2e-54 SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 31 0.41 SPAC1805.06c |hem2||porphobilinogen synthase Hem2 |Schizosacchar... 30 0.72 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 2.2 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 5.1 SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 27 5.1 SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 27 6.7 SPAPB8E5.03 |mae1||malic acid transport protein Mae1 |Schizosacc... 27 6.7 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 8.9 >SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces pombe|chr 2|||Manual Length = 251 Score = 248 bits (606), Expect = 2e-66 Identities = 106/184 (57%), Positives = 146/184 (79%), Gaps = 1/184 (0%) Frame = +3 Query: 132 RDEIRLARQARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRL 311 R++I L R+AR GNYYVP E KL FVIRIRG+N + PK RK++QL RL QINNGVFV+ Sbjct: 68 REQIELGRKARAEGNYYVPDETKLVFVIRIRGINNIPPKARKIMQLLRLIQINNGVFVKF 127 Query: 312 NKATVNMLRIAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNII 491 NKAT ML++ EPY+ +G PNLK+VREL+YKRGF K+N +R+ ++ N++IE L K +I+ Sbjct: 128 NKATKEMLQVVEPYVTYGIPNLKTVRELLYKRGFGKVNKQRIALSDNAIIEAALGKYSIL 187 Query: 492 CVEDLIHEIFTVGEKFKYASNFLWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKVNELL 668 +EDLIHEI+TVG FK A+NF+WPF+L++P GGWR +K H+++GGD G R++ +N L+ Sbjct: 188 SIEDLIHEIYTVGPNFKQAANFIWPFQLSSPLGGWRDRKFKHFIEGGDAGKRDEHINSLV 247 Query: 669 RRMV 680 R+M+ Sbjct: 248 RKML 251 >SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 245 bits (600), Expect = 1e-65 Identities = 105/184 (57%), Positives = 146/184 (79%), Gaps = 1/184 (0%) Frame = +3 Query: 132 RDEIRLARQARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRL 311 R++I LAR+AR GNY+VP E KL FV+RIRG+N + PK RK++QL RL QINNG+FV+ Sbjct: 67 REQIELARKARAEGNYFVPHEPKLIFVVRIRGINNIPPKARKIMQLLRLLQINNGIFVKF 126 Query: 312 NKATVNMLRIAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNII 491 NKA ML++ EPY+ +G PN K+VREL+YKRGF K+N +R+P++ N++IE L K +I+ Sbjct: 127 NKAIKEMLQVVEPYVTYGIPNHKTVRELIYKRGFGKVNKQRIPLSDNAIIEAALGKYSIL 186 Query: 492 CVEDLIHEIFTVGEKFKYASNFLWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKVNELL 668 VEDLIHEI+TVG FK A+NFLWPFKL++P GGWR +K H+++GGD G R++ +N L+ Sbjct: 187 SVEDLIHEIYTVGPNFKQAANFLWPFKLSSPLGGWRERKFKHFIEGGDAGKRDEHINGLV 246 Query: 669 RRMV 680 ++M+ Sbjct: 247 QKML 250 >SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 208 bits (508), Expect = 2e-54 Identities = 98/190 (51%), Positives = 131/190 (68%), Gaps = 1/190 (0%) Frame = +3 Query: 114 NTASRXRDEIRLARQARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINN 293 N R R+ IRL R A+N+G+ +VP E KL FVIRI GV + PK+RKVL+L RL +INN Sbjct: 60 NYRQRERERIRLNRSAKNKGDIFVPDETKLLFVIRIAGVKNMPPKIRKVLRLLRLSRINN 119 Query: 294 GVFVRLNKATVNMLRIAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRL 473 VFVR NKA MLRI EPY+ +G PNL SVREL+YKRGF K+NG+R+ ++ N+LIE+ L Sbjct: 120 AVFVRNNKAVAQMLRIVEPYVMYGIPNLHSVRELIYKRGFGKINGQRIALSDNALIEEAL 179 Query: 474 SKQNIICVEDLIHEIFTVGEKFKYASNFLWPFKLNNPTGG-WRKKTIHYVDGGDFGNRED 650 K ++I +ED+IHEI+ VG FK + FLWPF L KK H+ +G G + Sbjct: 180 GKYDVISIEDIIHEIYNVGSHFKEVTKFLWPFTLTPVKHSLMEKKVKHFNEGRKAGYCGE 239 Query: 651 KVNELLRRMV 680 ++NEL+++ V Sbjct: 240 EINELIKKQV 249 >SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor Sec74|Schizosaccharomyces pombe|chr 1|||Manual Length = 928 Score = 31.1 bits (67), Expect = 0.41 Identities = 15/35 (42%), Positives = 25/35 (71%) Frame = +1 Query: 454 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTS 558 HSS + L+N+ SSAL+++ + S +SSS+Q +S Sbjct: 90 HSSSQKLSNKVSSALKLTIPKRWRSSKSSSSQCSS 124 >SPAC1805.06c |hem2||porphobilinogen synthase Hem2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 30.3 bits (65), Expect = 0.72 Identities = 20/69 (28%), Positives = 36/69 (52%), Gaps = 2/69 (2%) Frame = +3 Query: 372 NLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLS--KQNIICVEDLIHEIFTVGEKFKY 545 N KSV + G L G ++ I+ + ++ R+ KQ ++ +E L H++ + K+ Sbjct: 143 NAKSVERIAEVSGNYALAGAQI-ISPSDCMDGRVKAIKQKLVELE-LSHKVCVISYSAKF 200 Query: 546 ASNFLWPFK 572 AS F PF+ Sbjct: 201 ASGFFGPFR 209 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 28.7 bits (61), Expect = 2.2 Identities = 30/105 (28%), Positives = 50/105 (47%) Frame = +1 Query: 259 YCSCSGCVRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVS 438 Y + +G +++T + S VS+ + S SP T T S S +++ S + + S S Sbjct: 116 YLTGNGVLQTTVSSSSVSSTTSSSS--SSSPSSSSTTTTTSPSSSSSSSSSSSS-SSSSS 172 Query: 439 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN 573 S + SS ++ +SS+ SS S SSS +TS S+ Sbjct: 173 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSS 217 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 27.5 bits (58), Expect = 5.1 Identities = 32/108 (29%), Positives = 50/108 (46%), Gaps = 7/108 (6%) Frame = +1 Query: 280 VRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TG-------SVS 438 V STTA S ST P T S + +TP V+ + T+A S T SVS Sbjct: 460 VNSTTATSASST-PL-TSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVS 517 Query: 439 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN*TT 582 + P++++ L + S+ +SS + +SST +TS + T+ Sbjct: 518 STAPSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATS 565 >SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium transporting Cta4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1211 Score = 27.5 bits (58), Expect = 5.1 Identities = 16/40 (40%), Positives = 24/40 (60%) Frame = +3 Query: 159 ARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRL 278 + N+G YY G +AFV I GV + SP++ LQL ++ Sbjct: 1133 SENKGMYY--GLLGIAFVA-IAGVTEFSPELNAKLQLVKM 1169 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 27.1 bits (57), Expect = 6.7 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +3 Query: 369 PNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNII 491 P LK+ REL+ K+ + V SN ++EK L +II Sbjct: 423 PKLKAERELLSKQESMEKQSMHVDEESNQILEKFLDVYDII 463 >SPAPB8E5.03 |mae1||malic acid transport protein Mae1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 27.1 bits (57), Expect = 6.7 Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 2/59 (3%) Frame = -1 Query: 610 IVFLRQPPVGLFSLNGHRKLLAYLNFSPTVNISWMRSSTQMMF--CLLSLFSMSELEVI 440 IV++ Q + LFSL G L ++ + T+ SW ++ CLLS+ + ++ I Sbjct: 68 IVYILQ--IFLFSLFGSCMLFRFIKYPSTIKDSWNHHLEKLFIATCLLSISTFIDMLAI 124 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 26.6 bits (56), Expect = 8.9 Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 2/100 (2%) Frame = +1 Query: 280 VRSTTACSFVSTRPQ*TC--FVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVSRSPPT 453 V ST++ +F S P T ++S S ++ ++ S S T++ + S S P T Sbjct: 189 VSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSS-----SLSTSSIPST 243 Query: 454 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN 573 SS ++ TSS+L SS S SSS+ + S S+ Sbjct: 244 SSS----SSSTSSSLSSSSSSSTASSSSSSSSIISSSSSS 279 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,782,230 Number of Sequences: 5004 Number of extensions: 86878 Number of successful extensions: 228 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 226 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 844406124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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