Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_D08_e156_08.seq
         (1513 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 ...    92   8e-19
At3g56450.1 68416.m06278 alpha-soluble NSF attachment protein 1 ...    69   6e-12
At4g37460.1 68417.m05302 tetratricopeptide repeat (TPR)-containi...    29   8.0  
At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl...    29   8.0  

>At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 /
           alpha-SNAP2 / ASNAP2 identical to alpha-soluble NSF
           attachment protein 2 / alpha-SNAP2 SP:Q9SPE6 from
           [Arabidopsis thaliana]
          Length = 289

 Score = 92.3 bits (219), Expect = 8e-19
 Identities = 40/95 (42%), Positives = 64/95 (67%), Gaps = 1/95 (1%)
 Frame = +3

Query: 72  HEGLLKYSAKEYMFRAALCHLC-VDLLNAQHALEKYAGLYPAFADTREYKLVKELIEHLE 248
           +  LLKY  K ++  A +CHLC  D+++  +ALEKY  L P F  TRE K + +L   ++
Sbjct: 184 NNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLASAID 243

Query: 249 EQNVDAFTEAVKTYDSISRLDQWYTTMLVRIKKQI 353
           E+++  FT+ VK +DS++ LD W TTML+R+K+++
Sbjct: 244 EEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKL 278


>At3g56450.1 68416.m06278 alpha-soluble NSF attachment protein 1 /
           alpha-SNAP1 (ASNAP1) identical to alpha-soluble NSF
           attachment protein 1 (Alpha-SNAP1) (N-
           ethylmaleimide-sensitive factor attachment protein,
           alpha 1) (Swiss-Prot:Q9LXZ5) [Arabidopsis thaliana]
          Length = 381

 Score = 69.3 bits (162), Expect = 6e-12
 Identities = 32/77 (41%), Positives = 49/77 (63%), Gaps = 1/77 (1%)
 Frame = +3

Query: 81  LLKYSAKEYMFRAALCHLC-VDLLNAQHALEKYAGLYPAFADTREYKLVKELIEHLEEQN 257
           LLKY  K ++  A +CHLC  D+++  +ALEKY  L P F+ TRE K + +L   ++E++
Sbjct: 238 LLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEED 297

Query: 258 VDAFTEAVKTYDSISRL 308
           +  FT+  K  DS+S L
Sbjct: 298 IAKFTDVSKEIDSVSPL 314


>At4g37460.1 68417.m05302 tetratricopeptide repeat (TPR)-containing
           protein contains Pfam profile PF00515: TPR Domain
          Length = 883

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 22/75 (29%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
 Frame = +3

Query: 45  YXPGLQXIRHE-GLLKYSAKEYMFRAALCHLCVDLLNAQHALEKYAGLYPAFADTREYKL 221
           + P    + HE G++ + +K+  F AA+  L + L   +     Y  L  AFA   EYK 
Sbjct: 394 FEPNSPDVLHERGIVNFKSKD--FTAAVKDLSICLKQEKDNKSAYTYLGLAFASLGEYKK 451

Query: 222 VKELIEHLEEQNVDA 266
            +E   HL+   +D+
Sbjct: 452 AEE--AHLKSIQLDS 464


>At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl
           CoA:diacylglycerol acyltransferase (DGAT) identical to
           gi:5050913, gi:6625553
          Length = 520

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 22/68 (32%), Positives = 35/68 (51%)
 Frame = -1

Query: 361 LSLICFLIRTSIVVYHWSSLDILSYVLTASVNASTFCSSRCSINSLTSLYSLVSAKAGYK 182
           L L+  ++   +V Y  +S DI S +  A+  A+   S   S+ SL   Y +V+    Y+
Sbjct: 241 LMLLTCIVWLKLVSYAHTSYDIRS-LANAADKANPEVSYYVSLKSLA--YFMVAPTLCYQ 297

Query: 181 PAYFSSAC 158
           P+Y  SAC
Sbjct: 298 PSYPRSAC 305


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,199,977
Number of Sequences: 28952
Number of extensions: 352125
Number of successful extensions: 683
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 665
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 681
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4038570048
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)