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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_D04_e124_08.seq
         (1507 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g41520.1 68418.m05044 40S ribosomal protein S10 (RPS10B) cont...   155   5e-38
At4g25740.1 68417.m03706 40S ribosomal protein S10 (RPS10A) 40S ...   142   4e-34
At5g52650.1 68418.m06536 40S ribosomal protein S10 (RPS10C) cont...   140   2e-33
At3g20780.1 68416.m02628 topoisomerase 6 subunit B (TOP6B) nearl...    30   3.4  
At3g49650.1 68416.m05426 kinesin motor protein-related several k...    29   8.0  

>At5g41520.1 68418.m05044 40S ribosomal protein S10 (RPS10B)
           contains similarity to 40S ribosomal protein S10
          Length = 180

 Score =  155 bits (377), Expect = 5e-38
 Identities = 80/161 (49%), Positives = 102/161 (63%), Gaps = 6/161 (3%)
 Frame = +1

Query: 115 MLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQF 294
           M++ + NR  I +YLFKEGV+ AKKD++ P+HP +E +PNLQVIK MQS KS+ YV+E F
Sbjct: 1   MIISETNRREISKYLFKEGVLFAKKDFNLPQHPLIESVPNLQVIKLMQSFKSKEYVRETF 60

Query: 295 AWRHFYWYLTNEGIEYLRIFLHLPPEIVPATLKRSVRTETVRRGAVG-RPDAP-----AR 456
           AW H+YW+LTNEGI++LR +L+LP EIVPATLK+  +      G  G RP  P      R
Sbjct: 61  AWMHYYWFLTNEGIDFLRTYLNLPSEIVPATLKKQQKPLGRPFGGGGDRPRGPPRGDGER 120

Query: 457 TAEDRSAYRRAPPAGAPHDKKADVGPGSADVEFRGGFGRGR 579
              DR  YR  P +G  +  KA   P      FRGG G  R
Sbjct: 121 RFGDRDGYRGGPKSGGEYGDKAG-APADYQPGFRGGAGGAR 160


>At4g25740.1 68417.m03706 40S ribosomal protein S10 (RPS10A) 40S
           ribosomal protein S10 - Lumbricus rubellus, PID:e1329701
          Length = 177

 Score =  142 bits (345), Expect = 4e-34
 Identities = 77/163 (47%), Positives = 98/163 (60%), Gaps = 9/163 (5%)
 Frame = +1

Query: 115 MLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQF 294
           M++ + NR  I +YLFKEGV  AKKD++ PKHP ++ +PNLQVIK MQS KS+ YV+E F
Sbjct: 1   MIISENNRREICKYLFKEGVCFAKKDFNLPKHPLID-VPNLQVIKLMQSFKSKEYVRETF 59

Query: 295 AWRHFYWYLTNEGIEYLRIFLHLPPEIVPATLKRSVR--------TETVRRGAVGRPDAP 450
           AW H+YW+LTNEGIE+LR +L+LP ++VPATLK+S +            R+    R D  
Sbjct: 60  AWMHYYWFLTNEGIEFLRTYLNLPSDVVPATLKKSAKPGGRPFGGPPGDRQRGPPRSDGD 119

Query: 451 ARTAEDRSAYRRAPPAG-APHDKKADVGPGSADVEFRGGFGRG 576
                DR  YR  P  G       AD  P       R GFGRG
Sbjct: 120 RPRFGDRDGYRGGPRGGDEKGGAPADFQPSFQGGGGRPGFGRG 162


>At5g52650.1 68418.m06536 40S ribosomal protein S10 (RPS10C)
           contains similarity to 40S ribosomal protein S10
          Length = 179

 Score =  140 bits (339), Expect = 2e-33
 Identities = 76/165 (46%), Positives = 98/165 (59%), Gaps = 11/165 (6%)
 Frame = +1

Query: 115 MLMPKQNRVSIYEYLFKEGVMVAKKDYHAPKHPDLEKIPNLQVIKAMQSLKSRGYVKEQF 294
           M++ + NR  I +YLFKEGV  AKKD++  KHP ++ +PNLQVIK MQS KS+ YV+E F
Sbjct: 1   MIISEANRKEICKYLFKEGVCFAKKDFNLAKHPLID-VPNLQVIKLMQSFKSKEYVRETF 59

Query: 295 AWRHFYWYLTNEGIEYLRIFLHLPPEIVPATLKRSVRTETVRRGAV--GRPDAPARTA-- 462
           AW H+YW+LTNEGIE+LR +L+LP ++VPATLK+S +      G     R   P      
Sbjct: 60  AWMHYYWFLTNEGIEFLRTYLNLPSDVVPATLKKSAKPGGRPFGGPPGDRSRGPRHEGGD 119

Query: 463 ----EDRSAYRRAPPAGAPHDKKADVGPGSADVEFRG---GFGRG 576
                DR  YR  P AG     +    P      F+G   GFGRG
Sbjct: 120 RPRFGDRDGYRAGPRAGGEFGGEKGGAPADYQPSFQGSGRGFGRG 164


>At3g20780.1 68416.m02628 topoisomerase 6 subunit B (TOP6B) nearly
           identical to topoisomerase 6 subunit B [Arabidopsis
           thaliana] GI:12331188
          Length = 670

 Score = 30.3 bits (65), Expect = 3.4
 Identities = 15/64 (23%), Positives = 31/64 (48%)
 Frame = -1

Query: 658 ELGTGPPLEXXXXXFTYIFLLKLRVQIDLFRNLL*IQHQLNQGRHQPSYHEELQLVVRAD 479
           ++ TG P+E      +  ++   R+ ID+ RN+  I     +G  +  +  E+Q+V+  +
Sbjct: 204 KMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGN 263

Query: 478 KLIY 467
              Y
Sbjct: 264 WTTY 267


>At3g49650.1 68416.m05426 kinesin motor protein-related several
           kinesin-like proteins
          Length = 813

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 20/67 (29%), Positives = 29/67 (43%), Gaps = 5/67 (7%)
 Frame = +1

Query: 397 SVRTETVRRGAVGRPDAPARTA-----EDRSAYRRAPPAGAPHDKKADVGPGSADVEFRG 561
           S R  TVR G+   P APA  +       +  +  +P +G    K +  G  S    +RG
Sbjct: 737 SPRAVTVRNGSYNTPLAPAAVSTKGARNQQRVFGTSPLSGTKGVKNSSYGQNSHTKLYRG 796

Query: 562 GFGRGRS 582
           G  +G S
Sbjct: 797 GGTKGHS 803


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,276,265
Number of Sequences: 28952
Number of extensions: 396955
Number of successful extensions: 979
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 917
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 976
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4019292864
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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