BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030712_D03_e116_07.seq (1484 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 151 2e-37 SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 145 1e-35 SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 27 5.0 SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 27 5.0 SPAC5D6.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 6.6 SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 27 8.8 >SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 151 bits (366), Expect = 2e-37 Identities = 78/124 (62%), Positives = 91/124 (73%), Gaps = 1/124 (0%) Frame = +1 Query: 49 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 228 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK EIREKLA+MYK + V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59 Query: 229 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRXQRXERKNRMXKVR 405 +T+FGGG+STGFALIYD+ + KKFEP +RL R G E ++ R QR +RKNR KV Sbjct: 60 LRTHFGGGRSTGFALIYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVF 119 Query: 406 GTKK 417 GT K Sbjct: 120 GTGK 123 >SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 145 bits (352), Expect = 1e-35 Identities = 73/124 (58%), Positives = 89/124 (71%), Gaps = 1/124 (0%) Frame = +1 Query: 49 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 228 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK ++REKL +MYK V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59 Query: 229 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRXQRXERKNRMXKVR 405 +T++GGG++TGFALIYD ++ KKFEP +RL R G E ++ R QR +RKNR KV Sbjct: 60 LRTHYGGGRTTGFALIYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVF 119 Query: 406 GTKK 417 GT K Sbjct: 120 GTGK 123 >SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 27.5 bits (58), Expect = 5.0 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +1 Query: 166 EIREKLAKMYKVTPDVVFVFGFKTNFGG 249 +++E LAK YK+ P +F F T+ G Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313 >SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 27.5 bits (58), Expect = 5.0 Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = -3 Query: 237 RLETEHENY-VWRHFVHFSELFTDLSFADGRFSWMQDIAYHL 115 RLE EH V R+ L +F D R+ W Q+I+ L Sbjct: 261 RLEVEHPGEEVLRNNRILGRLMPSRAFGDARYKWSQEISERL 302 >SPAC5D6.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 314 Score = 27.1 bits (57), Expect = 6.6 Identities = 12/19 (63%), Positives = 14/19 (73%) Frame = -1 Query: 59 PSLIFDYFRDFGVKTHVSP 3 P L+FDYF D G K HV+P Sbjct: 53 PDLLFDYFID-GHKIHVNP 70 >SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 518 Score = 26.6 bits (56), Expect = 8.8 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = -2 Query: 409 YLVPXSFCSYVPYAVXVSASSFHTGRDERVCVLAQTSL 296 Y VP + A+ V A H G+D+ C LA+ L Sbjct: 65 YDVPFLINDRIDVALAVGADGVHIGQDDMDCALARKIL 102 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,869,836 Number of Sequences: 5004 Number of extensions: 43038 Number of successful extensions: 120 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 108 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 828548732 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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