Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_C09_e163_05.seq
(1496 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 244 2e-65
SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|... 244 3e-65
SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom... 226 5e-60
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 31 0.41
SPAC1805.06c |hem2||porphobilinogen synthase Hem2 |Schizosacchar... 30 0.72
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 2.2
SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces... 29 2.2
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 2.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 5.1
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 27 5.1
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 27 6.7
>SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 244 bits (598), Expect = 2e-65
Identities = 116/242 (47%), Positives = 157/242 (64%)
Frame = +1
Query: 172 SKKLPAVPESVXXXXXXXXXXXXXXXQITLKRRSASIKKRKEIFKRAEQYVKEYRIKERD 351
SK+ PES+ ++++A KKR+ I KRAE Y EYR ER+
Sbjct: 10 SKEQIFAPESLLKKKKTQEQSREQRVAAAAEKKAAQQKKRELIAKRAESYDAEYRKAERE 69
Query: 352 EIRLARQARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNK 531
+I L R+AR GNYYVP E KL FVIRIRG+N + PK RK++QL RL QINNGVFV+ NK
Sbjct: 70 QIELGRKARAEGNYYVPDETKLVFVIRIRGINNIPPKARKIMQLLRLIQINNGVFVKFNK 129
Query: 532 ATVNMLRIAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICV 711
AT ML++ EPY+ +G PNLK+VREL+YKRGF K+N +R+ ++ N++IE L K +I+ +
Sbjct: 130 ATKEMLQVVEPYVTYGIPNLKTVRELLYKRGFGKVNKQRIALSDNAIIEAALGKYSILSI 189
Query: 712 EDLIHEIFTVGEKFKYASNFLWPFKLNNPTGGLAQEDDSXXXXXXXXXPARXKVNELLRR 891
EDLIHEI+TVG FK A+NF+WPF+L++P GG +N L+R+
Sbjct: 190 EDLIHEIYTVGPNFKQAANFIWPFQLSSPLGGWRDRKFKHFIEGGDAGKRDEHINSLVRK 249
Query: 892 MV 897
M+
Sbjct: 250 ML 251
>SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 250
Score = 244 bits (596), Expect = 3e-65
Identities = 114/235 (48%), Positives = 155/235 (65%)
Frame = +1
Query: 193 PESVXXXXXXXXXXXXXXXQITLKRRSASIKKRKEIFKRAEQYVKEYRIKERDEIRLARQ 372
PES+ +++SA KKR+ I KRAE Y EYR ER++I LAR+
Sbjct: 16 PESLLKKTKAQKQSREQIVAAAAEKKSARQKKRELIAKRAEAYEAEYRAAEREQIELARK 75
Query: 373 ARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLR 552
AR GNY+VP E KL FV+RIRG+N + PK RK++QL RL QINNG+FV+ NKA ML+
Sbjct: 76 ARAEGNYFVPHEPKLIFVVRIRGINNIPPKARKIMQLLRLLQINNGIFVKFNKAIKEMLQ 135
Query: 553 IAEPYIAWGYPNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEI 732
+ EPY+ +G PN K+VREL+YKRGF K+N +R+P++ N++IE L K +I+ VEDLIHEI
Sbjct: 136 VVEPYVTYGIPNHKTVRELIYKRGFGKVNKQRIPLSDNAIIEAALGKYSILSVEDLIHEI 195
Query: 733 FTVGEKFKYASNFLWPFKLNNPTGGLAQEDDSXXXXXXXXXPARXKVNELLRRMV 897
+TVG FK A+NFLWPFKL++P GG + +N L+++M+
Sbjct: 196 YTVGPNFKQAANFLWPFKLSSPLGGWRERKFKHFIEGGDAGKRDEHINGLVQKML 250
>SPAC664.06 |rpl703|rpl7|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 249
Score = 226 bits (553), Expect = 5e-60
Identities = 105/213 (49%), Positives = 143/213 (67%)
Frame = +1
Query: 259 LKRRSASIKKRKEIFKRAEQYVKEYRIKERDEIRLARQARNRGNYYVPGEAKLAFVIRIR 438
+ ++ A K RKE FKRAE ++ YR +ER+ IRL R A+N+G+ +VP E KL FVIRI
Sbjct: 37 IAKKEAQKKNRKETFKRAETFINNYRQRERERIRLNRSAKNKGDIFVPDETKLLFVIRIA 96
Query: 439 GVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNLKSVRELVYK 618
GV + PK+RKVL+L RL +INN VFVR NKA MLRI EPY+ +G PNL SVREL+YK
Sbjct: 97 GVKNMPPKIRKVLRLLRLSRINNAVFVRNNKAVAQMLRIVEPYVMYGIPNLHSVRELIYK 156
Query: 619 RGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEIFTVGEKFKYASNFLWPFKLNNP 798
RGF K+NG+R+ ++ N+LIE+ L K ++I +ED+IHEI+ VG FK + FLWPF L
Sbjct: 157 RGFGKINGQRIALSDNALIEEALGKYDVISIEDIIHEIYNVGSHFKEVTKFLWPFTLTPV 216
Query: 799 TGGLAQEDDSXXXXXXXXXPARXKVNELLRRMV 897
L ++ ++NEL+++ V
Sbjct: 217 KHSLMEKKVKHFNEGRKAGYCGEEINELIKKQV 249
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 31.1 bits (67), Expect = 0.41
Identities = 15/35 (42%), Positives = 25/35 (71%)
Frame = +2
Query: 668 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTS 772
HSS + L+N+ SSAL+++ + S +SSS+Q +S
Sbjct: 90 HSSSQKLSNKVSSALKLTIPKRWRSSKSSSSQCSS 124
>SPAC1805.06c |hem2||porphobilinogen synthase Hem2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 329
Score = 30.3 bits (65), Expect = 0.72
Identities = 20/69 (28%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
Frame = +1
Query: 586 NLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLS--KQNIICVEDLIHEIFTVGEKFKY 759
N KSV + G L G ++ I+ + ++ R+ KQ ++ +E L H++ + K+
Sbjct: 143 NAKSVERIAEVSGNYALAGAQI-ISPSDCMDGRVKAIKQKLVELE-LSHKVCVISYSAKF 200
Query: 760 ASNFLWPFK 786
AS F PF+
Sbjct: 201 ASGFFGPFR 209
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 28.7 bits (61), Expect = 2.2
Identities = 30/105 (28%), Positives = 50/105 (47%)
Frame = +2
Query: 473 YCSCSGCVRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVS 652
Y + +G +++T + S VS+ + S SP T T S S +++ S + + S S
Sbjct: 116 YLTGNGVLQTTVSSSSVSSTTSSSS--SSSPSSSSTTTTTSPSSSSSSSSSSSS-SSSSS 172
Query: 653 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN 787
S + SS ++ +SS+ SS S SSS +TS S+
Sbjct: 173 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSS 217
>SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 224
Score = 28.7 bits (61), Expect = 2.2
Identities = 11/38 (28%), Positives = 24/38 (63%)
Frame = +1
Query: 277 SIKKRKEIFKRAEQYVKEYRIKERDEIRLARQARNRGN 390
S+K+ +EI ++ E+ +R+K ++ ++ + A N GN
Sbjct: 152 SLKRNREIIEKEERSSFHFRVKPKNLDKVPKLAENEGN 189
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 28.3 bits (60), Expect = 2.9
Identities = 55/201 (27%), Positives = 77/201 (38%), Gaps = 9/201 (4%)
Frame = +2
Query: 494 VRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TG-------SVS 652
V STTA S ST P T S + +TP V+ + T+A S T SVS
Sbjct: 460 VNSTTATSASST-PL-TSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVS 517
Query: 653 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN*TTRLEVWRKKTIHY 832
+ P++++ L + S+ +SS + +SST +TS + T+
Sbjct: 518 STAPSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATS------------ 565
Query: 833 VDGGDFGNPRGTRSTS-SSGEWXXXXXXXXXXXXXXXTXGGAPDPISAXXXVVLQFTGRG 1009
FGN T S S S+GE+ T P ++ FT G
Sbjct: 566 ASSTPFGNSTITSSASGSTGEFTNTNSGNGDVSGSVTTPTSTP-LSNSTVAPTSTFTSSG 624
Query: 1010 F-TPSXLETLXXPTXXPLXXS 1069
F T S L T T PL S
Sbjct: 625 FNTTSGLPTSSAST--PLSNS 643
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.5 bits (58), Expect = 5.1
Identities = 41/167 (24%), Positives = 66/167 (39%), Gaps = 3/167 (1%)
Frame = +2
Query: 494 VRSTTACSFVSTRPQ*TC--FVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVSRSPPT 667
V ST++ +F S P T ++S S ++ ++ S S T++ + S S P T
Sbjct: 189 VSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSS-----SLSTSSIPST 243
Query: 668 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN*TTRLEVWRKKTIHYVDGGD 847
SS ++ TSS+L SS S SSS+ + S S+ ++ TI
Sbjct: 244 SSS----SSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTST--SSTIS-SSSSS 296
Query: 848 FGNPRGTRST-SSSGEWXXXXXXXXXXXXXXXTXGGAPDPISAXXXV 985
+P T ST SSS + + P S+ +
Sbjct: 297 SSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTI 343
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 27.5 bits (58), Expect = 5.1
Identities = 16/40 (40%), Positives = 24/40 (60%)
Frame = +1
Query: 373 ARNRGNYYVPGEAKLAFVIRIRGVNQVSPKVRKVLQLFRL 492
+ N+G YY G +AFV I GV + SP++ LQL ++
Sbjct: 1133 SENKGMYY--GLLGIAFVA-IAGVTEFSPELNAKLQLVKM 1169
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 27.1 bits (57), Expect = 6.7
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 583 PNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNII 705
P LK+ REL+ K+ + V SN ++EK L +II
Sbjct: 423 PKLKAERELLSKQESMEKQSMHVDEESNQILEKFLDVYDII 463
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,611,989
Number of Sequences: 5004
Number of extensions: 83452
Number of successful extensions: 239
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 836477428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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