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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_C04_e123_06.seq
         (1456 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC622.14 |||GTPase activating protein |Schizosaccharomyces pom...    58   4e-09
SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po...    48   3e-06
SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|ch...    46   1e-05
SPAC22E12.17c |glo3||ARF GTPase activating protein|Schizosacchar...    44   5e-05
SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||...    41   4e-04
SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3 |Schizos...    40   0.001
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    28   2.8  
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|...    27   6.5  
SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc...    27   8.6  
SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy...    27   8.6  

>SPCC622.14 |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 309

 Score = 57.6 bits (133), Expect = 4e-09
 Identities = 47/175 (26%), Positives = 80/175 (45%), Gaps = 18/175 (10%)
 Frame = +3

Query: 144 LEKMLVGGNAKAQEFFVSQPDYKAGMTIMQKYNTKAAAMYRQKIAALAEGHSWSPSDYKP 323
           ++ M VGGN+ A+ F  + P + A  +I +KYNT  A   RQKI A  +G  W   D +P
Sbjct: 67  VKMMEVGGNSNAKTFLSTDPMFSAAGSIREKYNTDIAEDLRQKIRAEVDGVEWVKVD-RP 125

Query: 324 EPIEKPPESQTRESYTSGDNVFQSGSENNISYHSEYGX--------------SRYTGFGN 461
           + +       +  +  +  +V     E N  Y ++ G                RY GFG+
Sbjct: 126 KSVSSHASVTSSSTVPTIPSV---SKEANDKYFAKLGSINSQRPDDLPPSQGGRYQGFGS 182

Query: 462 TPKQSQSTPMTPIHSGNEVVD----NTIATLATGWSMFTSSVSKAARTATENAVR 614
           +   S +   +  ++G   +D    N ++ L+ GW+MF+ SVS+  +   +   R
Sbjct: 183 S--NSVNPNSSARNNGGSFLDQLSSNPVSALSHGWNMFSRSVSQQIQDVQDPETR 235



 Score = 50.8 bits (116), Expect = 5e-07
 Identities = 20/33 (60%), Positives = 24/33 (72%)
 Frame = +2

Query: 50  ICLDCSGVHRSLGVHLSFXRSVTMDKWKDIXIR 148
           ICLDCSG HR LGV  SF RS+TMD W +  ++
Sbjct: 36  ICLDCSGQHRGLGVEKSFVRSITMDNWSERQVK 68


>SPAC824.09c |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 320

 Score = 48.0 bits (109), Expect = 3e-06
 Identities = 19/29 (65%), Positives = 23/29 (79%)
 Frame = +2

Query: 50  ICLDCSGVHRSLGVHLSFXRSVTMDKWKD 136
           IC+ CSGVHRSLGVH+S  +SV +D W D
Sbjct: 48  ICIRCSGVHRSLGVHVSRVKSVDLDSWTD 76


>SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 870

 Score = 46.0 bits (104), Expect = 1e-05
 Identities = 16/27 (59%), Positives = 24/27 (88%)
 Frame = +2

Query: 50  ICLDCSGVHRSLGVHLSFXRSVTMDKW 130
           +C+DCSG+HRSLG H++  RS+T+DK+
Sbjct: 706 LCIDCSGIHRSLGTHITKIRSLTLDKF 732


>SPAC22E12.17c |glo3||ARF GTPase activating
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 486

 Score = 44.0 bits (99), Expect = 5e-05
 Identities = 21/42 (50%), Positives = 26/42 (61%), Gaps = 5/42 (11%)
 Frame = +2

Query: 38  TAFGI--CLDCSGVHRSLGVHLS---FXRSVTMDKWKDIXIR 148
           T FGI  CLDCS  HR++GVH+S   F RS  +D W    +R
Sbjct: 37  TTFGIYLCLDCSAAHRNMGVHISFVRFLRSTVLDSWTYAQLR 78


>SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 923

 Score = 41.1 bits (92), Expect = 4e-04
 Identities = 14/25 (56%), Positives = 21/25 (84%)
 Frame = +2

Query: 50  ICLDCSGVHRSLGVHLSFXRSVTMD 124
           +C++CSG+HRSLG H+S  RS+ +D
Sbjct: 750 LCIECSGIHRSLGTHISKTRSLLLD 774


>SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 601

 Score = 39.5 bits (88), Expect = 0.001
 Identities = 13/34 (38%), Positives = 24/34 (70%)
 Frame = +2

Query: 50  ICLDCSGVHRSLGVHLSFXRSVTMDKWKDIXIRE 151
           +CL C+ +HR LG H+S  +S+++D+W +  I +
Sbjct: 42  LCLRCATIHRKLGTHVSKVKSISLDEWSNDQIEK 75


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 28.3 bits (60), Expect = 2.8
 Identities = 27/111 (24%), Positives = 46/111 (41%), Gaps = 7/111 (6%)
 Frame = +3

Query: 345  ESQTRESYTSGDNVFQSGSENNISYHSEYGXSRYTGFGNTPKQSQSTPMTPIHSGNE--- 515
            +S      +SGD    +    +IS  S+   S YT   N+ + S S P T   S +    
Sbjct: 728  QSSVSRPQSSGDASGFNTIFTSISQSSDGETSGYTISSNSSQNSASEPQTAFTSSSSSAT 787

Query: 516  --VVDNTIATLATGWSMFTSSVSK--AARTATENAVRYGGIASQKVSEMAS 656
              +  ++I+T  +  S   SS S   ++ + T +      IAS   + + S
Sbjct: 788  PTITQSSISTSVSSQSSMNSSYSSPISSNSVTSSTSIISSIASSSYTSIPS 838


>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1184

 Score = 27.1 bits (57), Expect = 6.5
 Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 3/50 (6%)
 Frame = +3

Query: 294 HSWSPSDYKPEPIEKPPESQTRESY---TSGDNVFQSGSENNISYHSEYG 434
           H   PS Y+ E  E+P E +   S+   +SG+N F+   E    Y   YG
Sbjct: 785 HETEPSHYEEEEEEEPEERREESSHFSESSGNNEFE---ETEQEYTHGYG 831


>SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 334

 Score = 26.6 bits (56), Expect = 8.6
 Identities = 28/125 (22%), Positives = 50/125 (40%), Gaps = 3/125 (2%)
 Frame = +3

Query: 297 SWSPSDYKPEPIEKPPESQTRESYTSGDNVFQSGSENNISYHSEYGXSRYTGFGNTPKQS 476
           S S S   P PI     S +  + TSG+N+        +        S   G  NT   S
Sbjct: 127 SISNSSESPAPINSATASMSPANNTSGNNITSPNVRGELDMSGNIAMS--GGPTNTASTS 184

Query: 477 QSTP--MTPIHSGNEVVDNTIATLATGWSMFTSSVS-KAARTATENAVRYGGIASQKVSE 647
              P  MT +   +    N +++ +   S  T+S +   + T T ++  + G   +  ++
Sbjct: 185 GPVPHDMTVLPQTDSNTSNLMSSGSQLGSFATASTNGNNSTTTTTSSAAHPGSFHKGTND 244

Query: 648 MASTV 662
            +ST+
Sbjct: 245 YSSTL 249


>SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 385

 Score = 26.6 bits (56), Expect = 8.6
 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = +3

Query: 525 NTIATLATGWSMFTSSV-SKAARTATENAVRYGGIASQKVSEMASTVTEKVXNS 683
           N+  +L      FTS+  S +    T + VRY  I S   SE  ST+T +  +S
Sbjct: 2   NSYISLIFTLLFFTSAARSSSVSVETGSCVRYTTIYSSGSSEFTSTITPETPSS 55


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,003,244
Number of Sequences: 5004
Number of extensions: 71847
Number of successful extensions: 205
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 812755802
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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