BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030712_C04_e123_06.seq (1456 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC622.14 |||GTPase activating protein |Schizosaccharomyces pom... 58 4e-09 SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po... 48 3e-06 SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|ch... 46 1e-05 SPAC22E12.17c |glo3||ARF GTPase activating protein|Schizosacchar... 44 5e-05 SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||... 41 4e-04 SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3 |Schizos... 40 0.001 SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 28 2.8 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 6.5 SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc... 27 8.6 SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy... 27 8.6 >SPCC622.14 |||GTPase activating protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 309 Score = 57.6 bits (133), Expect = 4e-09 Identities = 47/175 (26%), Positives = 80/175 (45%), Gaps = 18/175 (10%) Frame = +3 Query: 144 LEKMLVGGNAKAQEFFVSQPDYKAGMTIMQKYNTKAAAMYRQKIAALAEGHSWSPSDYKP 323 ++ M VGGN+ A+ F + P + A +I +KYNT A RQKI A +G W D +P Sbjct: 67 VKMMEVGGNSNAKTFLSTDPMFSAAGSIREKYNTDIAEDLRQKIRAEVDGVEWVKVD-RP 125 Query: 324 EPIEKPPESQTRESYTSGDNVFQSGSENNISYHSEYGX--------------SRYTGFGN 461 + + + + + +V E N Y ++ G RY GFG+ Sbjct: 126 KSVSSHASVTSSSTVPTIPSV---SKEANDKYFAKLGSINSQRPDDLPPSQGGRYQGFGS 182 Query: 462 TPKQSQSTPMTPIHSGNEVVD----NTIATLATGWSMFTSSVSKAARTATENAVR 614 + S + + ++G +D N ++ L+ GW+MF+ SVS+ + + R Sbjct: 183 S--NSVNPNSSARNNGGSFLDQLSSNPVSALSHGWNMFSRSVSQQIQDVQDPETR 235 Score = 50.8 bits (116), Expect = 5e-07 Identities = 20/33 (60%), Positives = 24/33 (72%) Frame = +2 Query: 50 ICLDCSGVHRSLGVHLSFXRSVTMDKWKDIXIR 148 ICLDCSG HR LGV SF RS+TMD W + ++ Sbjct: 36 ICLDCSGQHRGLGVEKSFVRSITMDNWSERQVK 68 >SPAC824.09c |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 48.0 bits (109), Expect = 3e-06 Identities = 19/29 (65%), Positives = 23/29 (79%) Frame = +2 Query: 50 ICLDCSGVHRSLGVHLSFXRSVTMDKWKD 136 IC+ CSGVHRSLGVH+S +SV +D W D Sbjct: 48 ICIRCSGVHRSLGVHVSRVKSVDLDSWTD 76 >SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 870 Score = 46.0 bits (104), Expect = 1e-05 Identities = 16/27 (59%), Positives = 24/27 (88%) Frame = +2 Query: 50 ICLDCSGVHRSLGVHLSFXRSVTMDKW 130 +C+DCSG+HRSLG H++ RS+T+DK+ Sbjct: 706 LCIDCSGIHRSLGTHITKIRSLTLDKF 732 >SPAC22E12.17c |glo3||ARF GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 486 Score = 44.0 bits (99), Expect = 5e-05 Identities = 21/42 (50%), Positives = 26/42 (61%), Gaps = 5/42 (11%) Frame = +2 Query: 38 TAFGI--CLDCSGVHRSLGVHLS---FXRSVTMDKWKDIXIR 148 T FGI CLDCS HR++GVH+S F RS +D W +R Sbjct: 37 TTFGIYLCLDCSAAHRNMGVHISFVRFLRSTVLDSWTYAQLR 78 >SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 923 Score = 41.1 bits (92), Expect = 4e-04 Identities = 14/25 (56%), Positives = 21/25 (84%) Frame = +2 Query: 50 ICLDCSGVHRSLGVHLSFXRSVTMD 124 +C++CSG+HRSLG H+S RS+ +D Sbjct: 750 LCIECSGIHRSLGTHISKTRSLLLD 774 >SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 601 Score = 39.5 bits (88), Expect = 0.001 Identities = 13/34 (38%), Positives = 24/34 (70%) Frame = +2 Query: 50 ICLDCSGVHRSLGVHLSFXRSVTMDKWKDIXIRE 151 +CL C+ +HR LG H+S +S+++D+W + I + Sbjct: 42 LCLRCATIHRKLGTHVSKVKSISLDEWSNDQIEK 75 >SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1131 Score = 28.3 bits (60), Expect = 2.8 Identities = 27/111 (24%), Positives = 46/111 (41%), Gaps = 7/111 (6%) Frame = +3 Query: 345 ESQTRESYTSGDNVFQSGSENNISYHSEYGXSRYTGFGNTPKQSQSTPMTPIHSGNE--- 515 +S +SGD + +IS S+ S YT N+ + S S P T S + Sbjct: 728 QSSVSRPQSSGDASGFNTIFTSISQSSDGETSGYTISSNSSQNSASEPQTAFTSSSSSAT 787 Query: 516 --VVDNTIATLATGWSMFTSSVSK--AARTATENAVRYGGIASQKVSEMAS 656 + ++I+T + S SS S ++ + T + IAS + + S Sbjct: 788 PTITQSSISTSVSSQSSMNSSYSSPISSNSVTSSTSIISSIASSSYTSIPS 838 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 27.1 bits (57), Expect = 6.5 Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 3/50 (6%) Frame = +3 Query: 294 HSWSPSDYKPEPIEKPPESQTRESY---TSGDNVFQSGSENNISYHSEYG 434 H PS Y+ E E+P E + S+ +SG+N F+ E Y YG Sbjct: 785 HETEPSHYEEEEEEEPEERREESSHFSESSGNNEFE---ETEQEYTHGYG 831 >SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 334 Score = 26.6 bits (56), Expect = 8.6 Identities = 28/125 (22%), Positives = 50/125 (40%), Gaps = 3/125 (2%) Frame = +3 Query: 297 SWSPSDYKPEPIEKPPESQTRESYTSGDNVFQSGSENNISYHSEYGXSRYTGFGNTPKQS 476 S S S P PI S + + TSG+N+ + S G NT S Sbjct: 127 SISNSSESPAPINSATASMSPANNTSGNNITSPNVRGELDMSGNIAMS--GGPTNTASTS 184 Query: 477 QSTP--MTPIHSGNEVVDNTIATLATGWSMFTSSVS-KAARTATENAVRYGGIASQKVSE 647 P MT + + N +++ + S T+S + + T T ++ + G + ++ Sbjct: 185 GPVPHDMTVLPQTDSNTSNLMSSGSQLGSFATASTNGNNSTTTTTSSAAHPGSFHKGTND 244 Query: 648 MASTV 662 +ST+ Sbjct: 245 YSSTL 249 >SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 385 Score = 26.6 bits (56), Expect = 8.6 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 1/54 (1%) Frame = +3 Query: 525 NTIATLATGWSMFTSSV-SKAARTATENAVRYGGIASQKVSEMASTVTEKVXNS 683 N+ +L FTS+ S + T + VRY I S SE ST+T + +S Sbjct: 2 NSYISLIFTLLFFTSAARSSSVSVETGSCVRYTTIYSSGSSEFTSTITPETPSS 55 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,003,244 Number of Sequences: 5004 Number of extensions: 71847 Number of successful extensions: 205 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 204 length of database: 2,362,478 effective HSP length: 75 effective length of database: 1,987,178 effective search space used: 812755802 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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