Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_B10_e170_04.seq
         (1496 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha...    29   2.2  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   8.8  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    27   8.8  

>SPCC1020.08 |||wybutosine biosynthesis protein
           Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 688

 Score = 28.7 bits (61), Expect = 2.2
 Identities = 13/41 (31%), Positives = 18/41 (43%)
 Frame = -3

Query: 432 NHCNVLITSWNTXXXXIKHSCFKNKFYDINNQLMNESIVSL 310
           +H  V I  W       +  C+K  FY I + L  E+  SL
Sbjct: 315 SHSGVKICRWTKSAMRGRGFCYKYSFYGIRSHLCMEATPSL 355


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 26.6 bits (56), Expect = 8.8
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +1

Query: 472 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 636
           P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231

Query: 637 SCA 645
           S A
Sbjct: 232 SDA 234


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 26.6 bits (56), Expect = 8.8
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +3

Query: 579 PPFASWRNSEEARTDRPSQQLRSLN 653
           PP AS RN  E  +  PS+Q  S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,667,573
Number of Sequences: 5004
Number of extensions: 83034
Number of successful extensions: 169
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 836477428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)