BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030712_B10_e170_04.seq (1496 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha ... 28 0.80 >AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha 1 chain precursor protein. Length = 801 Score = 27.9 bits (59), Expect = 0.80 Identities = 17/48 (35%), Positives = 19/48 (39%), Gaps = 2/48 (4%) Frame = -2 Query: 1069 GXRG--GAXGFXPXXSRXGPRAFPGQXQXRGPPXGVXI*GXXGPRXTP 932 G RG G G R GP PG +G V + G GPR P Sbjct: 438 GERGQMGPKGGQGVPGRPGPEGMPGDKGDKGESGSVGMPGPQGPRGYP 485 Score = 25.0 bits (52), Expect = 5.6 Identities = 12/39 (30%), Positives = 16/39 (41%) Frame = -2 Query: 1057 GAXGFXPXXSRXGPRAFPGQXQXRGPPXGVXI*GXXGPR 941 G G GPR FPG+ +G P + G G + Sbjct: 235 GLEGLPGPQGEVGPRGFPGRPGEKGVPGTPGVRGERGDK 273 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,246,257 Number of Sequences: 2352 Number of extensions: 24477 Number of successful extensions: 35 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 35 length of database: 563,979 effective HSP length: 67 effective length of database: 406,395 effective search space used: 175156245 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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