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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_B09_e162_03.seq
         (1546 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...    62   3e-08
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...    62   3e-08
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L...    62   3e-08
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...    62   3e-08
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    60   1e-07
UniRef50_Q17FQ0 Cluster: Putative uncharacterized protein; n=1; ...    48   7e-04
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:...    46   0.003
UniRef50_UPI0000E491E1 Cluster: PREDICTED: hypothetical protein;...    43   0.025
UniRef50_Q174L1 Cluster: Putative uncharacterized protein; n=1; ...    41   0.10 
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ...    40   0.18 
UniRef50_Q9VFX8 Cluster: CG15889-PA; n=3; Sophophora|Rep: CG1588...    37   1.2  
UniRef50_UPI0000D568DC Cluster: PREDICTED: hypothetical protein;...    37   1.6  
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba...    37   1.6  
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la...    36   2.9  
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba...    35   5.0  

>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score = 62.5 bits (145), Expect = 3e-08
 Identities = 27/27 (100%), Positives = 27/27 (100%)
 Frame = +3

Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
           LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 68  LAVVLQRRDWENPGVTQLNRLAAHPPF 94


>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score = 62.5 bits (145), Expect = 3e-08
 Identities = 27/27 (100%), Positives = 27/27 (100%)
 Frame = +3

Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
           LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 22  LAVVLQRRDWENPGVTQLNRLAAHPPF 48


>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
           protein - Phage M13mp18
          Length = 102

 Score = 62.5 bits (145), Expect = 3e-08
 Identities = 27/27 (100%), Positives = 27/27 (100%)
 Frame = +3

Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
           LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 26  LAVVLQRRDWENPGVTQLNRLAAHPPF 52


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score = 62.5 bits (145), Expect = 3e-08
 Identities = 27/27 (100%), Positives = 27/27 (100%)
 Frame = +3

Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
           LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 8   LAVVLQRRDWENPGVTQLNRLAAHPPF 34


>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 60.5 bits (140), Expect = 1e-07
 Identities = 26/27 (96%), Positives = 26/27 (96%)
 Frame = +1

Query: 622 HWPSFYNVGTGKTLALPNLIALQHIPL 702
           HWPSFYNV TGKTLALPNLIALQHIPL
Sbjct: 5   HWPSFYNVVTGKTLALPNLIALQHIPL 31


>UniRef50_Q17FQ0 Cluster: Putative uncharacterized protein; n=1;
           Aedes aegypti|Rep: Putative uncharacterized protein -
           Aedes aegypti (Yellowfever mosquito)
          Length = 275

 Score = 48.0 bits (109), Expect = 7e-04
 Identities = 23/43 (53%), Positives = 31/43 (72%)
 Frame = +2

Query: 221 ELDELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQQL 349
           ++DELD FF+ IS TV+KF+P      K QIF+LVS+ EL +L
Sbjct: 227 QMDELDGFFMQISRTVRKFSPRKIIELKMQIFNLVSQAELDEL 269


>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
           Beta-galactosidase - Yersinia pseudotuberculosis
          Length = 1066

 Score = 46.0 bits (104), Expect = 0.003
 Identities = 18/27 (66%), Positives = 21/27 (77%)
 Frame = +3

Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
           L  +L RRDWENP +TQ +RL AHPPF
Sbjct: 15  LPQILSRRDWENPQITQYHRLEAHPPF 41


>UniRef50_UPI0000E491E1 Cluster: PREDICTED: hypothetical protein;
           n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
           hypothetical protein - Strongylocentrotus purpuratus
          Length = 219

 Score = 42.7 bits (96), Expect = 0.025
 Identities = 24/50 (48%), Positives = 30/50 (60%)
 Frame = +2

Query: 200 QKKLPQRELDELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQQL 349
           Q  +PQ   DELD FF  IS TV+K TP LQ+  + QI  +V + EL  L
Sbjct: 166 QGSVPQE--DELDSFFKTISATVRKLTPVLQSQLRFQIMEMVYKAELGHL 213


>UniRef50_Q174L1 Cluster: Putative uncharacterized protein; n=1;
           Aedes aegypti|Rep: Putative uncharacterized protein -
           Aedes aegypti (Yellowfever mosquito)
          Length = 293

 Score = 40.7 bits (91), Expect = 0.10
 Identities = 20/52 (38%), Positives = 31/52 (59%), Gaps = 2/52 (3%)
 Frame = +2

Query: 227 DELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQQLA--PPSSTSSQ 376
           D +D F   I+ T+K   PY Q +A+++IF+ V + EL+ L   PP   S+Q
Sbjct: 238 DPVDAFLAGIAPTLKSLHPYYQNLARSEIFATVQKYELKMLTEPPPLCNSNQ 289


>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
           Enterobacter sakazakii ATCC BAA-894|Rep: Putative
           uncharacterized protein - Enterobacter sakazakii ATCC
           BAA-894
          Length = 1043

 Score = 39.9 bits (89), Expect = 0.18
 Identities = 14/26 (53%), Positives = 19/26 (73%)
 Frame = +3

Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPP 701
           LA +L R DW+NP +T +NRL +H P
Sbjct: 18  LATILARNDWQNPAITSVNRLPSHTP 43


>UniRef50_Q9VFX8 Cluster: CG15889-PA; n=3; Sophophora|Rep:
           CG15889-PA - Drosophila melanogaster (Fruit fly)
          Length = 359

 Score = 37.1 bits (82), Expect = 1.2
 Identities = 25/89 (28%), Positives = 42/89 (47%), Gaps = 4/89 (4%)
 Frame = +2

Query: 116 KAKEMSKSPQVLPVDATXAVLMARLLDEQKKLPQREL----DELDRFFLNISDTVKKFTP 283
           +A+E++    + P+  T +   AR L   +  P   +    D L+ FF +I  TVK   P
Sbjct: 238 QAQEINSKATITPIHTTPSYA-ARPLQSYQLQPLSPITPPRDSLELFFDSICATVKNLPP 296

Query: 284 YLQAVAKNQIFSLVSEMELQQLAPPSSTS 370
            L    K ++  L+ E+EL+ L    + S
Sbjct: 297 KLATEGKIRVMQLIGELELRALCEQEALS 325


>UniRef50_UPI0000D568DC Cluster: PREDICTED: hypothetical protein;
           n=1; Tribolium castaneum|Rep: PREDICTED: hypothetical
           protein - Tribolium castaneum
          Length = 365

 Score = 36.7 bits (81), Expect = 1.6
 Identities = 18/39 (46%), Positives = 23/39 (58%)
 Frame = +2

Query: 227 DELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQ 343
           D L  FF  +  T+KKF P LQ   K  +F +VSE EL+
Sbjct: 323 DTLKDFFDFLCKTIKKFPPQLQTQVKVDLFKIVSEAELR 361


>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
           Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
           pneumoniae
          Length = 1034

 Score = 36.7 bits (81), Expect = 1.6
 Identities = 15/24 (62%), Positives = 16/24 (66%)
 Frame = +3

Query: 633 VLQRRDWENPGVTQLNRLAAHPPF 704
           VL R DW N  +T LNRL AHP F
Sbjct: 17  VLAREDWHNQTITHLNRLPAHPVF 40


>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
           lactis|Rep: Beta-galactosidase - Lactococcus lactis
           subsp. lactis (Streptococcus lactis)
          Length = 998

 Score = 35.9 bits (79), Expect = 2.9
 Identities = 14/23 (60%), Positives = 17/23 (73%)
 Frame = +3

Query: 633 VLQRRDWENPGVTQLNRLAAHPP 701
           VL+R+DWENP V+  NRL  H P
Sbjct: 9   VLERKDWENPVVSNWNRLPMHTP 31


>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
           Gammaproteobacteria|Rep: Beta-galactosidase -
           Pseudoalteromonas haloplanktis (Alteromonas
           haloplanktis)
          Length = 1039

 Score = 35.1 bits (77), Expect = 5.0
 Identities = 12/23 (52%), Positives = 17/23 (73%)
 Frame = +3

Query: 633 VLQRRDWENPGVTQLNRLAAHPP 701
           ++ RRDWENP   Q+N++ AH P
Sbjct: 7   IINRRDWENPITVQVNQVKAHSP 29


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,028,200,126
Number of Sequences: 1657284
Number of extensions: 17606910
Number of successful extensions: 32246
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 31100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32224
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 165344584750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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