BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_B09_e162_03.seq
(1546 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 62 3e-08
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 62 3e-08
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 62 3e-08
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 62 3e-08
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 60 1e-07
UniRef50_Q17FQ0 Cluster: Putative uncharacterized protein; n=1; ... 48 7e-04
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:... 46 0.003
UniRef50_UPI0000E491E1 Cluster: PREDICTED: hypothetical protein;... 43 0.025
UniRef50_Q174L1 Cluster: Putative uncharacterized protein; n=1; ... 41 0.10
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ... 40 0.18
UniRef50_Q9VFX8 Cluster: CG15889-PA; n=3; Sophophora|Rep: CG1588... 37 1.2
UniRef50_UPI0000D568DC Cluster: PREDICTED: hypothetical protein;... 37 1.6
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba... 37 1.6
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la... 36 2.9
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba... 35 5.0
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 62.5 bits (145), Expect = 3e-08
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = +3
Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 68 LAVVLQRRDWENPGVTQLNRLAAHPPF 94
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 62.5 bits (145), Expect = 3e-08
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = +3
Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 22 LAVVLQRRDWENPGVTQLNRLAAHPPF 48
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 62.5 bits (145), Expect = 3e-08
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = +3
Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 26 LAVVLQRRDWENPGVTQLNRLAAHPPF 52
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 62.5 bits (145), Expect = 3e-08
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = +3
Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
LAVVLQRRDWENPGVTQLNRLAAHPPF
Sbjct: 8 LAVVLQRRDWENPGVTQLNRLAAHPPF 34
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba
histolytica HM-1:IMSS
Length = 86
Score = 60.5 bits (140), Expect = 1e-07
Identities = 26/27 (96%), Positives = 26/27 (96%)
Frame = +1
Query: 622 HWPSFYNVGTGKTLALPNLIALQHIPL 702
HWPSFYNV TGKTLALPNLIALQHIPL
Sbjct: 5 HWPSFYNVVTGKTLALPNLIALQHIPL 31
>UniRef50_Q17FQ0 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 275
Score = 48.0 bits (109), Expect = 7e-04
Identities = 23/43 (53%), Positives = 31/43 (72%)
Frame = +2
Query: 221 ELDELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQQL 349
++DELD FF+ IS TV+KF+P K QIF+LVS+ EL +L
Sbjct: 227 QMDELDGFFMQISRTVRKFSPRKIIELKMQIFNLVSQAELDEL 269
>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
Beta-galactosidase - Yersinia pseudotuberculosis
Length = 1066
Score = 46.0 bits (104), Expect = 0.003
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +3
Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPPF 704
L +L RRDWENP +TQ +RL AHPPF
Sbjct: 15 LPQILSRRDWENPQITQYHRLEAHPPF 41
>UniRef50_UPI0000E491E1 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 219
Score = 42.7 bits (96), Expect = 0.025
Identities = 24/50 (48%), Positives = 30/50 (60%)
Frame = +2
Query: 200 QKKLPQRELDELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQQL 349
Q +PQ DELD FF IS TV+K TP LQ+ + QI +V + EL L
Sbjct: 166 QGSVPQE--DELDSFFKTISATVRKLTPVLQSQLRFQIMEMVYKAELGHL 213
>UniRef50_Q174L1 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 293
Score = 40.7 bits (91), Expect = 0.10
Identities = 20/52 (38%), Positives = 31/52 (59%), Gaps = 2/52 (3%)
Frame = +2
Query: 227 DELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQQLA--PPSSTSSQ 376
D +D F I+ T+K PY Q +A+++IF+ V + EL+ L PP S+Q
Sbjct: 238 DPVDAFLAGIAPTLKSLHPYYQNLARSEIFATVQKYELKMLTEPPPLCNSNQ 289
>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 1043
Score = 39.9 bits (89), Expect = 0.18
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +3
Query: 624 LAVVLQRRDWENPGVTQLNRLAAHPP 701
LA +L R DW+NP +T +NRL +H P
Sbjct: 18 LATILARNDWQNPAITSVNRLPSHTP 43
>UniRef50_Q9VFX8 Cluster: CG15889-PA; n=3; Sophophora|Rep:
CG15889-PA - Drosophila melanogaster (Fruit fly)
Length = 359
Score = 37.1 bits (82), Expect = 1.2
Identities = 25/89 (28%), Positives = 42/89 (47%), Gaps = 4/89 (4%)
Frame = +2
Query: 116 KAKEMSKSPQVLPVDATXAVLMARLLDEQKKLPQREL----DELDRFFLNISDTVKKFTP 283
+A+E++ + P+ T + AR L + P + D L+ FF +I TVK P
Sbjct: 238 QAQEINSKATITPIHTTPSYA-ARPLQSYQLQPLSPITPPRDSLELFFDSICATVKNLPP 296
Query: 284 YLQAVAKNQIFSLVSEMELQQLAPPSSTS 370
L K ++ L+ E+EL+ L + S
Sbjct: 297 KLATEGKIRVMQLIGELELRALCEQEALS 325
>UniRef50_UPI0000D568DC Cluster: PREDICTED: hypothetical protein;
n=1; Tribolium castaneum|Rep: PREDICTED: hypothetical
protein - Tribolium castaneum
Length = 365
Score = 36.7 bits (81), Expect = 1.6
Identities = 18/39 (46%), Positives = 23/39 (58%)
Frame = +2
Query: 227 DELDRFFLNISDTVKKFTPYLQAVAKNQIFSLVSEMELQ 343
D L FF + T+KKF P LQ K +F +VSE EL+
Sbjct: 323 DTLKDFFDFLCKTIKKFPPQLQTQVKVDLFKIVSEAELR 361
>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
pneumoniae
Length = 1034
Score = 36.7 bits (81), Expect = 1.6
Identities = 15/24 (62%), Positives = 16/24 (66%)
Frame = +3
Query: 633 VLQRRDWENPGVTQLNRLAAHPPF 704
VL R DW N +T LNRL AHP F
Sbjct: 17 VLAREDWHNQTITHLNRLPAHPVF 40
>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
lactis|Rep: Beta-galactosidase - Lactococcus lactis
subsp. lactis (Streptococcus lactis)
Length = 998
Score = 35.9 bits (79), Expect = 2.9
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +3
Query: 633 VLQRRDWENPGVTQLNRLAAHPP 701
VL+R+DWENP V+ NRL H P
Sbjct: 9 VLERKDWENPVVSNWNRLPMHTP 31
>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
Gammaproteobacteria|Rep: Beta-galactosidase -
Pseudoalteromonas haloplanktis (Alteromonas
haloplanktis)
Length = 1039
Score = 35.1 bits (77), Expect = 5.0
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +3
Query: 633 VLQRRDWENPGVTQLNRLAAHPP 701
++ RRDWENP Q+N++ AH P
Sbjct: 7 IINRRDWENPITVQVNQVKAHSP 29
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,028,200,126
Number of Sequences: 1657284
Number of extensions: 17606910
Number of successful extensions: 32246
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 31100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32224
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 165344584750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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