Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_B07_e146_03.seq
(1430 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase... 44 4e-05
SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1 |S... 41 5e-04
SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizo... 36 0.014
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 36 0.014
SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosa... 34 0.042
SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac... 33 0.097
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 33 0.13
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 31 0.30
SPCC576.15c |ksg1||serine/threonine protein kinase Ksg1|Schizosa... 31 0.30
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 31 0.39
SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 31 0.52
SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1 |Schizosa... 30 0.68
SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase Wis1|Schizosaccha... 29 1.6
SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14 |Schizo... 28 2.8
SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase ... 28 3.6
SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 27 6.4
SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase Cka1|Sch... 27 8.4
>SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase
Cmk1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 335
Score = 44.4 bits (100), Expect = 4e-05
Identities = 29/89 (32%), Positives = 44/89 (49%)
Frame = +3
Query: 27 KYDFDDEAFNEISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQSALCTELHVTKTKLX 206
+Y F+D ++ IS+ AKDFI K L D RL A+ L HP+L + T ++
Sbjct: 249 EYTFNDPCWSGISETAKDFIKKCLENDPSKRLTAADALKHPFLSEKRPATS-NLLPNVRE 307
Query: 207 RYVIKKRWAKAVSAVIALKRMGAKFEDVH 293
+ +K + A +AV A K E+ H
Sbjct: 308 NFNARKTFRTAYNAVRAFNTW-KKLENKH 335
>SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 445
Score = 40.7 bits (91), Expect = 5e-04
Identities = 20/55 (36%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +3
Query: 33 DFDDEAFNEISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQ-SALCTELHVTK 194
DF D + +S AKD I LL + DR L+HPW + S+ T+L+ T+
Sbjct: 381 DFRDPVWKSVSRQAKDLISNLLKTNPPDRFTVKQCLSHPWFARHSSRLTKLYETR 435
>SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase
Srk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 580
Score = 35.9 bits (79), Expect = 0.014
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = +3
Query: 27 KYDFDDEAFNEISDDAKDFIGKLLVKDKDDRLQASATLTHPWL 155
+Y F +++IS AKD I LL D + R L HPW+
Sbjct: 379 EYSFLSPWWDDISKSAKDLISHLLTVDPESRYDIHQFLAHPWI 421
>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 504
Score = 35.9 bits (79), Expect = 0.014
Identities = 19/59 (32%), Positives = 28/59 (47%)
Frame = +3
Query: 27 KYDFDDEAFNEISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQSALCTELHVTKTKL 203
+++F +++ISD AKD I LL D +R HPW+ + E K KL
Sbjct: 309 EFEFLSPWWDDISDSAKDLITHLLTVDPRERYDIHQFFQHPWIKGESKMPENFTYKPKL 367
>SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase
Ark1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 355
Score = 34.3 bits (75), Expect = 0.042
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +3
Query: 21 VAKYDFDDEAFNEISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQ 161
+AK D +F + DA+D I +LL + + R+ + HPW+++
Sbjct: 298 IAKVDLKIPSF--VPPDARDLISRLLQHNPEKRMSLEQVMRHPWIVK 342
>SPCC18B5.11c |cds1||replication checkpoint kinase
Cds1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 33.1 bits (72), Expect = 0.097
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3
Query: 54 NEISDDAKDFIGKLLVKDKDDRLQASATLTHPW 152
NEIS++ D I ++L + + R+ S L HPW
Sbjct: 400 NEISEEGIDLINRMLEINPEKRISESEALQHPW 432
>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
Wis4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1401
Score = 32.7 bits (71), Expect = 0.13
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +3
Query: 57 EISDDAKDFIGKLLVKDKDDRLQASATLTHPWL 155
+IS A+DFI + +D + R +A LTHPW+
Sbjct: 1274 KISSLARDFIEQCFERDPEQRPRAVDLLTHPWI 1306
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 31.5 bits (68), Expect = 0.30
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +3
Query: 60 ISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQS 164
+S D K + ++LV D R L HPW+I++
Sbjct: 364 LSSDCKGLLSRMLVTDPLKRATLEEVLNHPWMIRN 398
>SPCC576.15c |ksg1||serine/threonine protein kinase
Ksg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 592
Score = 31.5 bits (68), Expect = 0.30
Identities = 16/32 (50%), Positives = 18/32 (56%)
Frame = +3
Query: 57 EISDDAKDFIGKLLVKDKDDRLQASATLTHPW 152
+ISD A D I KLLV D DRL HP+
Sbjct: 334 DISDVASDLIKKLLVLDPKDRLTVDEIHQHPF 365
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 31.1 bits (67), Expect = 0.39
Identities = 14/53 (26%), Positives = 27/53 (50%)
Frame = +3
Query: 54 NEISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQSALCTELHVTKTKLXRY 212
+ IS AK F+ + KD + R++ L HPW+I + ++ ++ +Y
Sbjct: 226 SNISSAAKSFLMQCFQKDPNLRIKTRKLLKHPWVIMNQTSSKFSDAIDEVQKY 278
>SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 372
Score = 30.7 bits (66), Expect = 0.52
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +3
Query: 51 FNEISDDAKDFIGKLLVKDKDDRLQASATLTHPWL 155
F + S DA D + KLL + D R+ A L HP++
Sbjct: 293 FPQASPDAIDLLEKLLTFNPDKRITAEEALKHPYV 327
>SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 652
Score = 30.3 bits (65), Expect = 0.68
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +3
Query: 57 EISDDAKDFIGKLLVKDKDDRLQASATLTHPWLI 158
+I ++ +D + +LL KD + R+ HPW +
Sbjct: 377 DIGEEGRDLLKRLLCKDPEQRITLVEVKLHPWTL 410
>SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase
Wis1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 605
Score = 29.1 bits (62), Expect = 1.6
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +3
Query: 54 NEISDDAKDFIGKLLVKDKDDRLQASATLTHPWLIQ 161
+ S +A+DF+ K L K+ R HPWL++
Sbjct: 546 DSFSPEARDFVNKCLNKNPSLRPDYHELANHPWLLK 581
>SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 566
Score = 28.3 bits (60), Expect = 2.8
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 60 ISDDAKDFIGKLLVKDKDDRLQASA 134
IS K+ I KLLVKD++DRL + A
Sbjct: 450 ISSLCKNLIRKLLVKDENDRLGSQA 474
>SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase
Ppk6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 27.9 bits (59), Expect = 3.6
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 57 EISDDAKDFIGKLLVKDKDDRLQASATLTHPW 152
E+S D D I ++L ++ DR+ TL H W
Sbjct: 726 ELSKDNVDLICRMLDRNVHDRITIEETLQHHW 757
>SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 532
Score = 27.1 bits (57), Expect = 6.4
Identities = 10/33 (30%), Positives = 18/33 (54%)
Frame = +3
Query: 60 ISDDAKDFIGKLLVKDKDDRLQASATLTHPWLI 158
+S A D I ++L+ R++ + + HPW I
Sbjct: 243 VSSSASDLIRQMLMVLPTSRIKVAEIMQHPWFI 275
>SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase
Cka1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 26.6 bits (56), Expect = 8.4
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 63 SDDAKDFIGKLLVKDKDDRLQASATLTHPW 152
+D+A D + +LL D +RL + HP+
Sbjct: 298 NDEAIDLLNRLLRYDHQERLTCQEAMAHPY 327
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,411,634
Number of Sequences: 5004
Number of extensions: 35450
Number of successful extensions: 113
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 796858378
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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