Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_B04_e122_04.seq
(1469 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60030.1 68418.m07527 expressed protein 36 0.089
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 34 0.21
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 33 0.47
At3g62620.1 68416.m07034 sucrose-phosphatase-related contains we... 31 1.9
At3g14350.3 68416.m01816 leucine-rich repeat transmembrane prote... 31 2.5
At3g14350.2 68416.m01814 leucine-rich repeat transmembrane prote... 31 2.5
At3g14350.1 68416.m01815 leucine-rich repeat transmembrane prote... 31 2.5
At1g13220.2 68414.m01534 nuclear matrix constituent protein-rela... 30 4.4
At4g39840.1 68417.m05645 expressed protein 29 7.7
At3g28770.1 68416.m03591 expressed protein 29 7.7
At2g04970.1 68415.m00518 hypothetical protein similar to At2g152... 29 7.7
>At5g60030.1 68418.m07527 expressed protein
Length = 292
Score = 35.5 bits (78), Expect = 0.089
Identities = 29/138 (21%), Positives = 54/138 (39%)
Frame = +1
Query: 115 RTGPRKRSYTQNDAEYASAKRKRLTEDMQLSVERLSRPRTTSATAKLSESTTPSKKEFTP 294
R +K+ D + K K ED Q S +R R + S K ++ +KE
Sbjct: 149 RKKEKKKKKNNKDEDVVDEKVKEKLEDEQKSADRKERKKKKSK--KNNDEDVVDEKEKLE 206
Query: 295 SKDKTGGKVSXTKLPNFAALHQKQFAKMESLDECQERKAKRARQLLSPVXXXXXXXXKNP 474
+ K+ K + + +K+ K+E ERK ++ ++ S
Sbjct: 207 DEQKSAEIKEKKKNKDEDVVDEKEKEKLEDEQRSGERKKEKKKKRKSDEEIVSEERKSKK 266
Query: 475 LKESQKDIQKPPRESTKK 528
++S +++ R+S KK
Sbjct: 267 KRKSDEEMGSEERKSKKK 284
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 34.3 bits (75), Expect = 0.21
Identities = 24/97 (24%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +1
Query: 145 QNDAEYASAKRKRLTEDMQLSVERLSRPRTTSATAKLSESTTPSKKE-FTPSKDKTGGKV 321
+ + E A+AK+K+ ++ + + + TS+ E S E P K GK
Sbjct: 345 EGEEETAAAKKKKKKKEKEKEKKAAAAAAATSSVEVKEEKQEESVTEPLQPKKKDAKGKA 404
Query: 322 SXTKLPNFAALHQKQFAKMESLDECQERKAKRARQLL 432
+ K+P Q+ A+ + E +ERK K + L
Sbjct: 405 AEKKIPKHVREMQEALARRQ---EAEERKKKEEEEKL 438
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 33.1 bits (72), Expect = 0.47
Identities = 23/92 (25%), Positives = 41/92 (44%)
Frame = +1
Query: 157 EYASAKRKRLTEDMQLSVERLSRPRTTSATAKLSESTTPSKKEFTPSKDKTGGKVSXTKL 336
E A+AK+K+ ++ ++ + T+S AK + + P K GK + K+
Sbjct: 304 ETAAAKKKKKKKEKDKE-KKAAAAATSSVEAKEEKQEESVTEPLQPKKKDAKGKAAEKKI 362
Query: 337 PNFAALHQKQFAKMESLDECQERKAKRARQLL 432
P Q+ A+ + E +ERK K + L
Sbjct: 363 PKHVREMQEALARRQ---EAEERKKKEEEEKL 391
>At3g62620.1 68416.m07034 sucrose-phosphatase-related contains weak
similarity to sucrose-phosphatase [Arabidopsis thaliana]
gi|11127757|gb|AAG31075
Length = 358
Score = 31.1 bits (67), Expect = 1.9
Identities = 19/73 (26%), Positives = 38/73 (52%), Gaps = 4/73 (5%)
Frame = +1
Query: 175 RKRLTEDMQLSVERLSRPRTTSATAKLSESTTPSKKE----FTPSKDKTGGKVSXTKLPN 342
+K+ T+ +L+ + +S+P+ + ++S KK +P+KD T KVS +K
Sbjct: 131 KKKKTKKQELASDSISKPKLLTEKPEVSWKVNGKKKTEANASSPAKDATDSKVSFSKPTK 190
Query: 343 FAALHQKQFAKME 381
++ Q Q +K +
Sbjct: 191 SSSQVQDQKSKSQ 203
>At3g14350.3 68416.m01816 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 1 GB:AAC27894 from [Zea
mays]
Length = 689
Score = 30.7 bits (66), Expect = 2.5
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +1
Query: 469 NPLKESQKDIQKPPRESTKKLDFGMKPSTLPSQAER 576
N + K +Q PP TKKLD + + P +ER
Sbjct: 341 NDFHQENKSVQNPPLVETKKLDTSLSMNLRPPPSER 376
>At3g14350.2 68416.m01814 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 1 GB:AAC27894 from [Zea
mays]
Length = 680
Score = 30.7 bits (66), Expect = 2.5
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +1
Query: 469 NPLKESQKDIQKPPRESTKKLDFGMKPSTLPSQAER 576
N + K +Q PP TKKLD + + P +ER
Sbjct: 304 NDFHQENKSVQNPPLVETKKLDTSLSMNLRPPPSER 339
>At3g14350.1 68416.m01815 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 1 GB:AAC27894 from [Zea
mays]
Length = 717
Score = 30.7 bits (66), Expect = 2.5
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +1
Query: 469 NPLKESQKDIQKPPRESTKKLDFGMKPSTLPSQAER 576
N + K +Q PP TKKLD + + P +ER
Sbjct: 341 NDFHQENKSVQNPPLVETKKLDTSLSMNLRPPPSER 376
>At1g13220.2 68414.m01534 nuclear matrix constituent protein-related
similar to nuclear matrix constituent protein 1 (NMCP1)
[Daucus carota] GI:2190187
Length = 1128
Score = 29.9 bits (64), Expect = 4.4
Identities = 25/76 (32%), Positives = 35/76 (46%), Gaps = 4/76 (5%)
Frame = +1
Query: 115 RTGPRKRSYTQNDAEYASAKRKRLTEDMQLSVERLS-RPRTTSATAKLSESTTPSKKEFT 291
R P S + D EY ++ + +E QLS + S R R AK + + T S K +
Sbjct: 881 RLRPSSSSIPEEDEEYTDSRVQETSEGSQLSEFQSSRRGRGRPRKAKPALNPTSSVKHAS 940
Query: 292 ---PSKDKTGGKVSXT 330
SKD+ G VS T
Sbjct: 941 LEESSKDELSGHVSVT 956
>At4g39840.1 68417.m05645 expressed protein
Length = 451
Score = 29.1 bits (62), Expect = 7.7
Identities = 36/164 (21%), Positives = 67/164 (40%), Gaps = 2/164 (1%)
Frame = +1
Query: 19 SSSXHNXKCKVIKSSLKG-SNKSFLFQESDSPARTGPRKRSYTQNDAEYASAKRKRLTED 195
SSS + K+ K++ G S+K + S + +T + N ++ + +
Sbjct: 103 SSSSTKNQTKLAKTTTMGTSHKLNSTKSSSNTTKTSSELKKL--NSGTKSTNSTSSIKKS 160
Query: 196 MQLSVERLSRPRTTSATAKLSESTTPSKKEFTP-SKDKTGGKVSXTKLPNFAALHQKQFA 372
LS S+ +TT S+ PS+K+ P SK T K S ++ F ++
Sbjct: 161 ADLSKSSSSKNKTTIKPPSSKLSSPPSEKKSQPSSKPVTKSKQSEKEIKPFWLDDEED-- 218
Query: 373 KMESLDECQERKAKRARQLLSPVXXXXXXXXKNPLKESQKDIQK 504
+ + E ++ + R L+ P KN + ++ K I K
Sbjct: 219 -EDFVSEFRDLPTRFQRSLI-PDLERISTTSKNYINKANKQITK 260
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 29.1 bits (62), Expect = 7.7
Identities = 38/175 (21%), Positives = 64/175 (36%), Gaps = 5/175 (2%)
Frame = +1
Query: 19 SSSXHNXKCKVIKSSLKGSNKSFLFQESDSPARTGPRKRSYTQNDAEYASAK---RKRLT 189
++ N K K K +S + + K+S T+ +A+ K +KR
Sbjct: 981 TTKSENSKLKEENKDNKEKKESEDSASKNREKKEYEEKKSKTKEEAKKEKKKSQDKKREE 1040
Query: 190 EDMQLSVERLSRPRTTSATAKLSESTTPSKKEFT--PSKDKTGGKVSXTKLPNFAALHQK 363
+D + + + + AK E T KKE SK K K +K
Sbjct: 1041 KDSEERKSKKEKEESRDLKAKKKEEETKEKKESENHKSKKKEDKKEHEDNKSMKKEEDKK 1100
Query: 364 QFAKMESLDECQERKAKRARQLLSPVXXXXXXXXKNPLKESQKDIQKPPRESTKK 528
+ K E ++ + K+ + L KN K+SQ ++ +ES KK
Sbjct: 1101 EKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQ-HVKLVKKESDKK 1154
>At2g04970.1 68415.m00518 hypothetical protein similar to At2g15200,
At1g32830, At2g14140, At3g30450, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 808
Score = 29.1 bits (62), Expect = 7.7
Identities = 38/191 (19%), Positives = 72/191 (37%)
Frame = +1
Query: 7 EAQPSSSXHNXKCKVIKSSLKGSNKSFLFQESDSPARTGPRKRSYTQNDAEYASAKRKRL 186
E Q + KV +G+ K + ++ D K+ + E ++
Sbjct: 515 EKQEEEGKEEEEEKVEYRGDEGTEKQEIPKQGDEEMEGEEEKQEEEGKEEEEEKVLKEES 574
Query: 187 TEDMQLSVERLSRPRTTSATAKLSESTTPSKKEFTPSKDKTGGKVSXTKLPNFAALHQKQ 366
E+ E + + TTP++KE P K++T T++P +
Sbjct: 575 VEEHDEHDETEDQEAYVILSDDEDNGTTPTEKESQPQKEET------TEVP--------K 620
Query: 367 FAKMESLDECQERKAKRARQLLSPVXXXXXXXXKNPLKESQKDIQKPPRESTKKLDFGMK 546
+E DE E + + A +LS + + +++ + PRE TKK D +
Sbjct: 621 EENVEEHDEHDETEDQEAYVILSDDEDNGTAPTEKESQPQKEETTEVPRE-TKKDDEDVN 679
Query: 547 PSTLPSQAERL 579
+ L +Q E +
Sbjct: 680 QTPLSTQEEEI 690
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,992,979
Number of Sequences: 28952
Number of extensions: 274591
Number of successful extensions: 971
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 918
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 961
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3903629760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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