Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_B03_e114_03.seq
(1559 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 217 5e-57
SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|... 215 1e-56
SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom... 180 4e-46
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 31 0.43
SPAC1805.06c |hem2||porphobilinogen synthase Hem2 |Schizosacchar... 30 0.75
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 2.3
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 5.3
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 27 7.0
SPAPB8E5.03 |mae1||malic acid transport protein Mae1 |Schizosacc... 27 7.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 9.3
>SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 217 bits (529), Expect = 5e-57
Identities = 92/160 (57%), Positives = 128/160 (80%), Gaps = 1/160 (0%)
Frame = +3
Query: 78 KLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNL 257
KL FVIRIRG+N + PK RK++QL RL QINNGVFV+ NKAT ML++ EPY+ +G PNL
Sbjct: 90 KLVFVIRIRGINNIPPKARKIMQLLRLIQINNGVFVKFNKATKEMLQVVEPYVTYGIPNL 149
Query: 258 KSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEIFTVGEKFKYASNF 437
K+VREL+YKRGF K+N +R+ ++ N++IE L K +I+ +EDLIHEI+TVG FK A+NF
Sbjct: 150 KTVRELLYKRGFGKVNKQRIALSDNAIIEAALGKYSILSIEDLIHEIYTVGPNFKQAANF 209
Query: 438 LWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKVNELLRR 554
+WPF+L++P GGWR +K H+++GGD G R++ +N L+R+
Sbjct: 210 IWPFQLSSPLGGWRDRKFKHFIEGGDAGKRDEHINSLVRK 249
>SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 250
Score = 215 bits (525), Expect = 1e-56
Identities = 91/160 (56%), Positives = 127/160 (79%), Gaps = 1/160 (0%)
Frame = +3
Query: 78 KLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNL 257
KL FV+RIRG+N + PK RK++QL RL QINNG+FV+ NKA ML++ EPY+ +G PN
Sbjct: 89 KLIFVVRIRGINNIPPKARKIMQLLRLLQINNGIFVKFNKAIKEMLQVVEPYVTYGIPNH 148
Query: 258 KSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEIFTVGEKFKYASNF 437
K+VREL+YKRGF K+N +R+P++ N++IE L K +I+ VEDLIHEI+TVG FK A+NF
Sbjct: 149 KTVRELIYKRGFGKVNKQRIPLSDNAIIEAALGKYSILSVEDLIHEIYTVGPNFKQAANF 208
Query: 438 LWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKVNELLRR 554
LWPFKL++P GGWR +K H+++GGD G R++ +N L+++
Sbjct: 209 LWPFKLSSPLGGWRERKFKHFIEGGDAGKRDEHINGLVQK 248
>SPAC664.06 |rpl703|rpl7|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 249
Score = 180 bits (439), Expect = 4e-46
Identities = 85/162 (52%), Positives = 113/162 (69%), Gaps = 1/162 (0%)
Frame = +3
Query: 78 KLAFVIRIRGVNQVSPKVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNL 257
KL FVIRI GV + PK+RKVL+L RL +INN VFVR NKA MLRI EPY+ +G PNL
Sbjct: 88 KLLFVIRIAGVKNMPPKIRKVLRLLRLSRINNAVFVRNNKAVAQMLRIVEPYVMYGIPNL 147
Query: 258 KSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNIICVEDLIHEIFTVGEKFKYASNF 437
SVREL+YKRGF K+NG+R+ ++ N+LIE+ L K ++I +ED+IHEI+ VG FK + F
Sbjct: 148 HSVRELIYKRGFGKINGQRIALSDNALIEEALGKYDVISIEDIIHEIYNVGSHFKEVTKF 207
Query: 438 LWPFKLNNPTGG-WRKKTIHYVDGGDFGNREDKVNELLRRXV 560
LWPF L KK H+ +G G +++NEL+++ V
Sbjct: 208 LWPFTLTPVKHSLMEKKVKHFNEGRKAGYCGEEINELIKKQV 249
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 31.1 bits (67), Expect = 0.43
Identities = 15/35 (42%), Positives = 25/35 (71%)
Frame = +1
Query: 334 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTS 438
HSS + L+N+ SSAL+++ + S +SSS+Q +S
Sbjct: 90 HSSSQKLSNKVSSALKLTIPKRWRSSKSSSSQCSS 124
>SPAC1805.06c |hem2||porphobilinogen synthase Hem2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 329
Score = 30.3 bits (65), Expect = 0.75
Identities = 20/69 (28%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
Frame = +3
Query: 252 NLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLS--KQNIICVEDLIHEIFTVGEKFKY 425
N KSV + G L G ++ I+ + ++ R+ KQ ++ +E L H++ + K+
Sbjct: 143 NAKSVERIAEVSGNYALAGAQI-ISPSDCMDGRVKAIKQKLVELE-LSHKVCVISYSAKF 200
Query: 426 ASNFLWPFK 452
AS F PF+
Sbjct: 201 ASGFFGPFR 209
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 28.7 bits (61), Expect = 2.3
Identities = 30/105 (28%), Positives = 50/105 (47%)
Frame = +1
Query: 139 YCSCSGCVRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVS 318
Y + +G +++T + S VS+ + S SP T T S S +++ S + + S S
Sbjct: 116 YLTGNGVLQTTVSSSSVSSTTSSSS--SSSPSSSSTTTTTSPSSSSSSSSSSSS-SSSSS 172
Query: 319 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN 453
S + SS ++ +SS+ SS S SSS +TS S+
Sbjct: 173 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSS 217
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.5 bits (58), Expect = 5.3
Identities = 32/108 (29%), Positives = 50/108 (46%), Gaps = 7/108 (6%)
Frame = +1
Query: 160 VRSTTACSFVSTRPQ*TCFVSLSPILHGATPT*RVSESWCTNAVSPN*TG-------SVS 318
V STTA S ST P T S + +TP V+ + T+A S T SVS
Sbjct: 460 VNSTTATSASST-PL-TSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVS 517
Query: 319 RSPPTHSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN*TT 462
+ P++++ L + S+ +SS + +SST +TS + T+
Sbjct: 518 STAPSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATS 565
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 27.1 bits (57), Expect = 7.0
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +3
Query: 249 PNLKSVRELVYKRGFAKLNGKRVPITSNSLIEKRLSKQNII 371
P LK+ REL+ K+ + V SN ++EK L +II
Sbjct: 423 PKLKAERELLSKQESMEKQSMHVDEESNQILEKFLDVYDII 463
>SPAPB8E5.03 |mae1||malic acid transport protein Mae1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 438
Score = 27.1 bits (57), Expect = 7.0
Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Frame = -2
Query: 490 IVFLRQPPVGLFSLNGHRKLLAYLNFSPTVNISWMRSSTQMMF--CLLSLFSMSELEVI 320
IV++ Q + LFSL G L ++ + T+ SW ++ CLLS+ + ++ I
Sbjct: 68 IVYILQ--IFLFSLFGSCMLFRFIKYPSTIKDSWNHHLEKLFIATCLLSISTFIDMLAI 124
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 9.3
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 2/100 (2%)
Frame = +1
Query: 160 VRSTTACSFVSTRPQ*TC--FVSLSPILHGATPT*RVSESWCTNAVSPN*TGSVSRSPPT 333
V ST++ +F S P T ++S S ++ ++ S S T++ + S S P T
Sbjct: 189 VSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSS-----SLSTSSIPST 243
Query: 334 HSSRRDLANRTSSALRISSMRYLLSVRSSSTQVTSCGHSN 453
SS ++ TSS+L SS S SSS+ + S S+
Sbjct: 244 SSS----SSSTSSSLSSSSSSSTASSSSSSSSIISSSSSS 279
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,579,318
Number of Sequences: 5004
Number of extensions: 57425
Number of successful extensions: 174
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 878103082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -