BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030712_A08_e153_02.seq
(1506 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_3140| Best HMM Match : C1_3 (HMM E-Value=7) 32 1.4
SB_57323| Best HMM Match : ShTK (HMM E-Value=0) 29 7.3
SB_3211| Best HMM Match : Keratin_B2 (HMM E-Value=0.83) 29 9.7
>SB_3140| Best HMM Match : C1_3 (HMM E-Value=7)
Length = 340
Score = 31.9 bits (69), Expect = 1.4
Identities = 20/66 (30%), Positives = 27/66 (40%), Gaps = 4/66 (6%)
Frame = +2
Query: 53 PGLQXIRHEXTGCEITSSSFTRNIMYSYIRYIVHDILAAFSPAY*KPLTCQLVKC----I 220
PG T C +FTR +Y+ Y VH + A SP L C +C +
Sbjct: 100 PGAHSFHCAFTQCHSLHCAFTRCTLYALRIYPVHTLCTALSPGA-HSLHCAFTRCTLYAL 158
Query: 221 YIYLIH 238
IY +H
Sbjct: 159 RIYPVH 164
>SB_57323| Best HMM Match : ShTK (HMM E-Value=0)
Length = 911
Score = 29.5 bits (63), Expect = 7.3
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = -3
Query: 721 FLHECTCTAAVLPRFQSGTIKMHVFYIQPNQFSCV 617
+ H T A P SGT H+F+ P+ SC+
Sbjct: 204 YFHSTTDQTATTPSTFSGTTSSHLFFQMPDMSSCL 238
>SB_3211| Best HMM Match : Keratin_B2 (HMM E-Value=0.83)
Length = 533
Score = 29.1 bits (62), Expect = 9.7
Identities = 11/35 (31%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Frame = +2
Query: 428 HTYICS--ETSSGSSAGRVCTRQCAKCSFILYYIH 526
H +C+ + +G S+ +C C +C I+YY+H
Sbjct: 228 HHPLCALRDAFNGVSSSIMCITGCIQCRVIIYYVH 262
Score = 29.1 bits (62), Expect = 9.7
Identities = 12/37 (32%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = +2
Query: 422 SLHTYICS--ETSSGSSAGRVCTRQCAKCSFILYYIH 526
S H +CS + +G S+ +C C + I+YY+H
Sbjct: 294 SCHFVLCSLRDAFNGVSSSSICITGCLQWRAIIYYVH 330
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 31,150,794
Number of Sequences: 59808
Number of extensions: 510183
Number of successful extensions: 711
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 711
length of database: 16,821,457
effective HSP length: 85
effective length of database: 11,737,777
effective search space used: 4882915232
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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