BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030712_A08_e153_02.seq (1506 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_3140| Best HMM Match : C1_3 (HMM E-Value=7) 32 1.4 SB_57323| Best HMM Match : ShTK (HMM E-Value=0) 29 7.3 SB_3211| Best HMM Match : Keratin_B2 (HMM E-Value=0.83) 29 9.7 >SB_3140| Best HMM Match : C1_3 (HMM E-Value=7) Length = 340 Score = 31.9 bits (69), Expect = 1.4 Identities = 20/66 (30%), Positives = 27/66 (40%), Gaps = 4/66 (6%) Frame = +2 Query: 53 PGLQXIRHEXTGCEITSSSFTRNIMYSYIRYIVHDILAAFSPAY*KPLTCQLVKC----I 220 PG T C +FTR +Y+ Y VH + A SP L C +C + Sbjct: 100 PGAHSFHCAFTQCHSLHCAFTRCTLYALRIYPVHTLCTALSPGA-HSLHCAFTRCTLYAL 158 Query: 221 YIYLIH 238 IY +H Sbjct: 159 RIYPVH 164 >SB_57323| Best HMM Match : ShTK (HMM E-Value=0) Length = 911 Score = 29.5 bits (63), Expect = 7.3 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = -3 Query: 721 FLHECTCTAAVLPRFQSGTIKMHVFYIQPNQFSCV 617 + H T A P SGT H+F+ P+ SC+ Sbjct: 204 YFHSTTDQTATTPSTFSGTTSSHLFFQMPDMSSCL 238 >SB_3211| Best HMM Match : Keratin_B2 (HMM E-Value=0.83) Length = 533 Score = 29.1 bits (62), Expect = 9.7 Identities = 11/35 (31%), Positives = 20/35 (57%), Gaps = 2/35 (5%) Frame = +2 Query: 428 HTYICS--ETSSGSSAGRVCTRQCAKCSFILYYIH 526 H +C+ + +G S+ +C C +C I+YY+H Sbjct: 228 HHPLCALRDAFNGVSSSIMCITGCIQCRVIIYYVH 262 Score = 29.1 bits (62), Expect = 9.7 Identities = 12/37 (32%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = +2 Query: 422 SLHTYICS--ETSSGSSAGRVCTRQCAKCSFILYYIH 526 S H +CS + +G S+ +C C + I+YY+H Sbjct: 294 SCHFVLCSLRDAFNGVSSSSICITGCLQWRAIIYYVH 330 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 31,150,794 Number of Sequences: 59808 Number of extensions: 510183 Number of successful extensions: 711 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 603 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 711 length of database: 16,821,457 effective HSP length: 85 effective length of database: 11,737,777 effective search space used: 4882915232 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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