Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_A03_e113_01.seq
         (1504 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...    99   8e-22
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    34   0.059
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   1.3  
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces...    28   3.8  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    28   3.8  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    27   5.1  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   6.7  
SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces pomb...    27   6.7  
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|...    27   8.9  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score =   99 bits (238), Expect = 8e-22
 Identities = 55/140 (39%), Positives = 81/140 (57%)
 Frame = +1

Query: 115 SGVTVSDACKTTYEEIKKDKKHRYVXFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTG 294
           SGV VS  C   ++E+K  K  RYV F + D K   V      + ++D+FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60

Query: 295 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 474
           +CRY ++DFE+ +  +G      + K+  +SW PD A +K KM+YSSS D L+++  G+ 
Sbjct: 61  DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114

Query: 475 KYIQATDLSEASQEAVEEKL 534
             IQATD SE + E V EK+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 33.9 bits (74), Expect = 0.059
 Identities = 15/27 (55%), Positives = 21/27 (77%)
 Frame = +1

Query: 358 SKKQKLFLMSWCPDTAKVKKKMLYSSS 438
           SKK  L L+S+ P+ A V++KMLY+SS
Sbjct: 76  SKKNLLQLISYVPENANVRRKMLYASS 102



 Score = 28.7 bits (61), Expect = 2.2
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +1

Query: 370 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 522
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 346 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 525
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 526 EKL 534
           E L
Sbjct: 138 EFL 140


>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 446

 Score = 27.9 bits (59), Expect = 3.8
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = -2

Query: 435 ARVQHLLLDLGGVWAPRH*EQFLFLARLRCTLALMRVFKIEE 310
           A ++ LLL +     PR  ++ +   R+ CT  L  VF++EE
Sbjct: 93  ASLEELLLKIFN--GPRSDKELILTIRIMCTFCLASVFQVEE 132


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 27.9 bits (59), Expect = 3.8
 Identities = 16/54 (29%), Positives = 25/54 (46%)
 Frame = -3

Query: 389 HDIRNNFCFLLASDVPWH*CVYSKSKRPYLHSPVPPFCRSSRKLSYSALRPPTV 228
           HD+ ++  +++ S   +H C  ++S  P  HS  PP            LRPP V
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFP--HSHHPPLHNPLPVSCQPVLRPPPV 158


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 27.5 bits (58), Expect = 5.1
 Identities = 27/136 (19%), Positives = 61/136 (44%), Gaps = 1/136 (0%)
 Frame = +1

Query: 142 KTTYEEIKKDKKHRYVXFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTGECRYGLFDF 321
           K + + ++ + K ++     +D K+ D + +  +  EY SF ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 322 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLS 501
           +   +  G    S+K    ++      A V K      ++   +K++    K ++  +  
Sbjct: 167 Q--KKVHGPPALSEKPGKCILK-DKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEFE 223

Query: 502 EASQ-EAVEEKLRATD 546
             ++ E  EEK + ++
Sbjct: 224 SINEGETEEEKAKTSN 239


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 6.7
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +1

Query: 436 SFDALKKSLVGVQKYIQATDLSEASQ 513
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


>SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 515

 Score = 27.1 bits (57), Expect = 6.7
 Identities = 18/57 (31%), Positives = 23/57 (40%)
 Frame = +1

Query: 628 CHIPWFETAAPQYTPTGGAPQLTLKQTPLLGSLQRAGAPSSLFNGWCSLLHNKRFPF 798
           C    F    P Y     A +L LK    L +LQ+    +     + S LHNK  PF
Sbjct: 12  CSCRGFSVCTP-YASIKSASELALKNISELQALQKIQNHTEKLKFFSSKLHNKGEPF 67


>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 584

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 17/41 (41%), Positives = 22/41 (53%)
 Frame = +1

Query: 121 VTVSDACKTTYEEIKKDKKHRYVXFYIRDEKQIDVETVGGR 243
           VT  D  KT  +  +K  KH+ V  YIR  K +D   +GGR
Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVD---IGGR 149


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,298,944
Number of Sequences: 5004
Number of extensions: 81781
Number of successful extensions: 258
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 241
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 253
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 840441776
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)