Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030712_A01_e097_01.seq
         (1414 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa...    85   2e-17
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    29   2.1  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   8.3  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    27   8.3  

>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
           L22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 85.0 bits (201), Expect = 2e-17
 Identities = 43/99 (43%), Positives = 58/99 (58%), Gaps = 1/99 (1%)
 Frame = +2

Query: 224 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNNLGNHVVIARD-KTKVAINADI 400
           +S K+ ID T    D I DV  FEKYL +R+KV+GKT NLG+ VV++R+  +K+A+ A I
Sbjct: 8   VSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAVIAHI 67

Query: 401 PFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDSYELRYF 517
            FS                  DWLRVV++    YELRY+
Sbjct: 68  DFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVYELRYY 106


>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 19/62 (30%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
 Frame = -2

Query: 354 T*LPRLFVLPSTLTRSFKYFSKLPTSRMLSSAGCVQSMVNLRLIF-LLAPLPRILPPFTP 178
           T +P+L  L   +T  +  F++    R+    G ++++  L ++F +L  L RI+ PFTP
Sbjct: 714 TVVPQLLGLIEEMTNWYIRFNR---RRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTP 770

Query: 177 FL 172
           F+
Sbjct: 771 FI 772


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 26.6 bits (56), Expect = 8.3
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +3

Query: 627 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 791
           P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231

Query: 792 SCA 800
           S A
Sbjct: 232 SDA 234


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 26.6 bits (56), Expect = 8.3
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +2

Query: 734 PPFASWRNSEEARTDRPSQQLRSLN 808
           PP AS RN  E  +  PS+Q  S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,344,185
Number of Sequences: 5004
Number of extensions: 78443
Number of successful extensions: 162
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 784935310
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)