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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_H11_e88_15.seq
         (1515 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g16570.1 68414.m01986 glycosyl transferase family 1 protein c...   125   7e-29
At1g26680.1 68414.m03250 transcriptional factor B3 family protei...    31   2.6  
At2g28220.1 68415.m03426 aspartyl protease family protein contai...    29   8.0  
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ...    29   8.0  
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ...    29   8.0  

>At1g16570.1 68414.m01986 glycosyl transferase family 1 protein
           contains similarity to mannosyltransferase GI:1800223
           from Dictyostelium discoideum; contains Pfam glycosyl
           transferase, group 1 family protein domain PF00534
          Length = 465

 Score =  125 bits (302), Expect = 7e-29
 Identities = 74/189 (39%), Positives = 104/189 (55%), Gaps = 6/189 (3%)
 Frame = +3

Query: 204 VVVLADIGRSPRMQYHALSLANNG-FKVNIITYVETTPLTEITENPNIQISKL-HPLDYN 377
           VVVL D+GRSPRMQYHALSLA    F+V+I+ Y  + P   +  +P+I I  +  P    
Sbjct: 9   VVVLGDLGRSPRMQYHALSLARQASFQVDIVAYGGSIPHEAVLNHPSIHIHTMAQPRFIQ 68

Query: 378 KGPQLLQYVA---KTIWQSISLLLTLFISGKC-HYLLCQNPPAIPTLPICSVYCLVARVQ 545
             P++L  V    K   Q   LL  LF+        L QNPP++PTL          R  
Sbjct: 69  YFPKILYPVTLLLKAFIQFTMLLWFLFVKVPAPDIFLVQNPPSVPTLIAVKWASSWRRAA 128

Query: 546 LIIDWHNYGYSLMAMSLHRDHLLVLXATHIEKLFGQYAHHNFLCHLCMKEDLLQNWNIVA 725
            ++DWHN+GY+L+A+SL R++LLV      E  +G+ A  +      M+ +L QNW + A
Sbjct: 129 FVVDWHNFGYTLLALSLGRNNLLVSLYRWSENHYGKMATGSLCVTKAMQHELDQNWGVRA 188

Query: 726 TTLYDRPPK 752
             LYD+PP+
Sbjct: 189 KVLYDQPPE 197


>At1g26680.1 68414.m03250 transcriptional factor B3 family protein
           low similarity to reproductive meristem gene 1 from
           [Brassica oleracea var. botrytis] GI:3170424,
           [Arabidopsis thaliana] GI:13604227; contains Pfam
           profile PF02362: B3 DNA binding domain
          Length = 920

 Score = 30.7 bits (66), Expect = 2.6
 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
 Frame = +2

Query: 392 TSICSENYM-AKHKFAFNLIYKWKMPLFAVSEPTSHSNITHMQC 520
           TS C  N + A   F F L+  WK P+ ++    S+++ T ++C
Sbjct: 329 TSFCQVNRIKAGDSFKFKLVGTWKKPVLSLCPTQSNNHKTPLEC 372


>At2g28220.1 68415.m03426 aspartyl protease family protein contains
           Pfam domain, PF00026: eukaryotic aspartyl protease
          Length = 756

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 24/90 (26%), Positives = 38/90 (42%)
 Frame = +3

Query: 471 LLCQNPPAIPTLPICSVYCLVARVQLIIDWHNYGYSLMAMSLHRDHLLVLXATHIEKLFG 650
           +LC     I   P+ +++       L++D +N      +  L    ++    T  E +FG
Sbjct: 330 MLCYFSETIDIFPVITMH-FSGGADLVLDKYNMYMESNSGGLFCLAIICNSPTQ-EAIFG 387

Query: 651 QYAHHNFLCHLCMKEDLLQNWNIVATTLYD 740
             A +NFL        LLQ  +  A TLYD
Sbjct: 388 NRAQNNFLVGYDSSSLLLQGASPYADTLYD 417


>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
           identical to SUMO activating enzyme 2 [Arabidopsis
           thaliana] GI:22652854; contains Pfam profiles PF00899:
           ThiF family, PF02134: Repeat in ubiquitin-activating
           (UBA) protein
          Length = 700

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = +3

Query: 438 LTLFISGKCHYLLCQNPPAIPTLPICSVYCLVARVQLIIDW 560
           +T+ I GK     CQ  PA  T P+C++     +    I W
Sbjct: 143 VTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKFVHCIVW 183


>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
           identical to SUMO activating enzyme 2 [Arabidopsis
           thaliana] GI:22652854; contains Pfam profiles PF00899:
           ThiF family, PF02134: Repeat in ubiquitin-activating
           (UBA) protein
          Length = 625

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = +3

Query: 438 LTLFISGKCHYLLCQNPPAIPTLPICSVYCLVARVQLIIDW 560
           +T+ I GK     CQ  PA  T P+C++     +    I W
Sbjct: 143 VTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKFVHCIVW 183


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,998,633
Number of Sequences: 28952
Number of extensions: 491569
Number of successful extensions: 946
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 940
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4048208640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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