BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_H07_e56_15.seq (1507 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 142 1e-34 SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces ... 116 8e-27 >SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 437 Score = 142 bits (343), Expect = 1e-34 Identities = 67/150 (44%), Positives = 95/150 (63%) Frame = +2 Query: 251 GXPFVLPSVRQAEEILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQNKSNCTVQT 430 G P+VLPSVRQAE L + L+ Y PI+G +G+ + I+++ + Q+ Sbjct: 79 GKPYVLPSVRQAETELLSQKLDKEYAPITGIPSFRVQATKLAYGDVYESIKDRL-VSAQS 137 Query: 431 LSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDL 610 +SGTGAL + F+ + Y +K I++ PTWGNH + + L K Y+Y+DP T G D+ Sbjct: 138 ISGTGALCIAANFLASFYP-SKTIYVSDPTWGNHKNVFSRAGLTVKSYKYYDPATRGLDI 196 Query: 611 QGALEDISKIPEGSIILLHACAHNPTGVDP 700 +G L D++ P+GSIILLHACAHNPTGVDP Sbjct: 197 KGMLSDLTSAPDGSIILLHACAHNPTGVDP 226 Score = 52.0 bits (119), Expect = 2e-07 Identities = 23/33 (69%), Positives = 25/33 (75%) Frame = +1 Query: 133 AXVPMGPPDVILGITEAYKKDTHXNKVNLGVGS 231 A VPMGPPD I GITEAYKKD K+NLG G+ Sbjct: 41 ADVPMGPPDPIFGITEAYKKDGDVKKMNLGAGT 73 Score = 27.1 bits (57), Expect = 6.7 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +3 Query: 879 FAKNMGLYGXPAXALTFXSXTLNSXAKVLS 968 FAKNMGLYG A + + A++ S Sbjct: 282 FAKNMGLYGERAGCFSILANDAEEAARIES 311 >SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 116 bits (279), Expect = 8e-27 Identities = 61/161 (37%), Positives = 86/161 (53%), Gaps = 3/161 (1%) Frame = +2 Query: 227 GAYXR**XGXPFVLPSVRQAEEILXKKG-LNHGYPPISGXXXXXXXXXXXXFGEDSQVIQ 403 GAY R G P++LP+V++A +I+ ++ NH Y PI+G F + ++ Sbjct: 38 GAY-RDDTGKPWILPAVKKASKIVEEQASFNHEYLPIAGLPRFTKAAAEVLFRPNPHLLS 96 Query: 404 NKSNCTVQTLSGTGALRLGLEFITNHYAK--AKEIWMPTPTWGNHPQICNMLKLPHKKYR 577 ++Q++SGTGA L FI Y K +++ PTW H + L + Y Sbjct: 97 EDRVASMQSVSGTGANFLAASFIETFYVKHTGAHVYISNPTWPVHRTLWEKLGVTVDTYP 156 Query: 578 YFDPKTNGFDLQGALEDISKIPEGSIILLHACAHNPTGVDP 700 Y+D K FD +G L I PEGSI LLHACAHNPTG+DP Sbjct: 157 YWDAKNRSFDYEGMLSTIKSAPEGSIFLLHACAHNPTGIDP 197 Score = 28.7 bits (61), Expect = 2.2 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +3 Query: 879 FAKNMGLYGXPAXALTFXSXTLNSXAKVLS 968 FAKNMGLYG + + + ++ KVLS Sbjct: 253 FAKNMGLYGERTGCMHYVAKDASTKNKVLS 282 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,961,718 Number of Sequences: 5004 Number of extensions: 70895 Number of successful extensions: 114 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 104 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 110 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 842423950 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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