Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_H07_e56_15.seq
         (1507 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast...   150   2e-36
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast...   150   2e-36
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic...   145   6e-35
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast...   142   5e-34
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic...   140   3e-33
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic...   137   2e-32
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri...   134   1e-31
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica...    31   2.6  
At4g18240.1 68417.m02709 starch synthase-related protein contain...    29   8.0  

>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
           transaminase A (ASP5) (AAT1) nearly identical to
           SP|P46248 Aspartate aminotransferase, chloroplast
           precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
           thaliana}
          Length = 453

 Score =  150 bits (364), Expect = 2e-36
 Identities = 69/152 (45%), Positives = 94/152 (61%)
 Frame = +2

Query: 257 PFVLPSVRQAEEILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQNKSNCTVQTLS 436
           P+VL  V++AE ++ ++G N  Y PI G            FG    VI+ +   T+Q LS
Sbjct: 94  PYVLNVVKKAENLMLERGDNKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLS 153

Query: 437 GTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQG 616
           GTG+LRL    I  ++  AK + + +PTWGNH  I N  K+P  +YRY+DPKT G D +G
Sbjct: 154 GTGSLRLAAALIERYFPGAKVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEG 212

Query: 617 ALEDISKIPEGSIILLHACAHNPTGVDPRPQR 712
            + DI + PEGS ILLH CAHNPTG+DP P++
Sbjct: 213 MIADIKEAPEGSFILLHGCAHNPTGIDPTPEQ 244



 Score = 44.8 bits (101), Expect = 1e-04
 Identities = 18/31 (58%), Positives = 25/31 (80%)
 Frame = +1

Query: 139 VPMGPPDVILGITEAYKKDTHXNKVNLGVGS 231
           + M PPD ILG++EA+K DT+  K+NLGVG+
Sbjct: 56  ITMAPPDPILGVSEAFKADTNGMKLNLGVGA 86


>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
           transaminase A (ASP5) (AAT1) nearly identical to
           SP|P46248 Aspartate aminotransferase, chloroplast
           precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
           thaliana}
          Length = 453

 Score =  150 bits (364), Expect = 2e-36
 Identities = 69/152 (45%), Positives = 94/152 (61%)
 Frame = +2

Query: 257 PFVLPSVRQAEEILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQNKSNCTVQTLS 436
           P+VL  V++AE ++ ++G N  Y PI G            FG    VI+ +   T+Q LS
Sbjct: 94  PYVLNVVKKAENLMLERGDNKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLS 153

Query: 437 GTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQG 616
           GTG+LRL    I  ++  AK + + +PTWGNH  I N  K+P  +YRY+DPKT G D +G
Sbjct: 154 GTGSLRLAAALIERYFPGAKVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEG 212

Query: 617 ALEDISKIPEGSIILLHACAHNPTGVDPRPQR 712
            + DI + PEGS ILLH CAHNPTG+DP P++
Sbjct: 213 MIADIKEAPEGSFILLHGCAHNPTGIDPTPEQ 244



 Score = 44.8 bits (101), Expect = 1e-04
 Identities = 18/31 (58%), Positives = 25/31 (80%)
 Frame = +1

Query: 139 VPMGPPDVILGITEAYKKDTHXNKVNLGVGS 231
           + M PPD ILG++EA+K DT+  K+NLGVG+
Sbjct: 56  ITMAPPDPILGVSEAFKADTNGMKLNLGVGA 86


>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
           isozyme 1 / transaminase A (ASP2) identical to SP|P46645
           Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
           2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
          Length = 405

 Score =  145 bits (352), Expect = 6e-35
 Identities = 75/163 (46%), Positives = 96/163 (58%), Gaps = 1/163 (0%)
 Frame = +2

Query: 227 GAYXR**XGXPFVLPSVRQAEEILXKKGLN-HGYPPISGXXXXXXXXXXXXFGEDSQVIQ 403
           GAY R   G P VL  VR+AE+ L         Y PI G             G DS  I 
Sbjct: 37  GAY-RTEEGKPLVLDVVRKAEQQLVNDPSRVKEYIPIVGISDFNKLSAKLILGADSPAIT 95

Query: 404 NKSNCTVQTLSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYF 583
                TVQ LSGTG+LR+G EF+  HY ++  I++P PTWGNHP++ N+  L  + +RY+
Sbjct: 96  ESRVTTVQCLSGTGSLRVGAEFLKTHYHQSV-IYIPKPTWGNHPKVFNLAGLSVEYFRYY 154

Query: 584 DPKTNGFDLQGALEDISKIPEGSIILLHACAHNPTGVDPRPQR 712
           DP T G D +G LED+   P G+I+LLHACAHNPTGVDP  ++
Sbjct: 155 DPATRGLDFKGLLEDLGAAPSGAIVLLHACAHNPTGVDPTSEQ 197



 Score = 35.1 bits (77), Expect = 0.12
 Identities = 16/31 (51%), Positives = 19/31 (61%)
 Frame = +1

Query: 139 VPMGPPDVILGITEAYKKDTHXNKVNLGVGS 231
           V   P D ILG+T AY  D    K+NLGVG+
Sbjct: 8   VARAPEDPILGVTVAYNNDPSPVKINLGVGA 38


>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
           transaminase A (ASP3) (YLS4) identical to SP|P46644
           Aspartate aminotransferase, chloroplast precursor (EC
           2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
           identical to cDNA YLS4 mRNA for aspartate
           aminotransferase (ASP3), partial cds GI:13122285
          Length = 449

 Score =  142 bits (344), Expect = 5e-34
 Identities = 76/163 (46%), Positives = 96/163 (58%), Gaps = 1/163 (0%)
 Frame = +2

Query: 227 GAYXR**XGXPFVLPSVRQAEE-ILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQ 403
           GAY R   G P VL  VR+AE+ ++  +     Y PI G             G DS  I+
Sbjct: 81  GAY-RTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAKLILGADSPAIR 139

Query: 404 NKSNCTVQTLSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYF 583
                TV+ LSGTG+LR+G EF+  HY + K I++  PTWGNHP+I  +  L  K YRY+
Sbjct: 140 ENRITTVECLSGTGSLRVGGEFLAKHYHQ-KTIYITQPTWGNHPKIFTLAGLTVKTYRYY 198

Query: 584 DPKTNGFDLQGALEDISKIPEGSIILLHACAHNPTGVDPRPQR 712
           DP T G + QG LED+     GSI+LLHACAHNPTGVDP  Q+
Sbjct: 199 DPATRGLNFQGLLEDLGAAAPGSIVLLHACAHNPTGVDPTIQQ 241



 Score = 35.5 bits (78), Expect = 0.092
 Identities = 16/27 (59%), Positives = 19/27 (70%)
 Frame = +1

Query: 151 PPDVILGITEAYKKDTHXNKVNLGVGS 231
           P D ILG+T AY KD    K+NLGVG+
Sbjct: 56  PEDPILGVTVAYNKDPSPVKLNLGVGA 82


>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
           isozyme 2 / transaminase A (ASP4) identical to aspartate
           aminotransferase, cytoplasmic isozyme 2 SP:P46646
           [Arabidopsis thaliana]
          Length = 403

 Score =  140 bits (338), Expect = 3e-33
 Identities = 67/150 (44%), Positives = 91/150 (60%)
 Frame = +2

Query: 251 GXPFVLPSVRQAEEILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQNKSNCTVQT 430
           G P VL  VR+AE+ L    L+  Y P++G             G+DS  ++     T Q 
Sbjct: 44  GKPLVLDVVRRAEQQLAND-LDKEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQC 102

Query: 431 LSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDL 610
           LSGTG+LR+G EF+  H  K   I++P PTWGNHP+I  +  L  + +RY+DPK+ G D 
Sbjct: 103 LSGTGSLRVGAEFLATHN-KESVIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDF 161

Query: 611 QGALEDISKIPEGSIILLHACAHNPTGVDP 700
           +G LED+   P G+I++L ACAHNPTGVDP
Sbjct: 162 KGMLEDLGAAPPGAIVVLQACAHNPTGVDP 191


>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
           isozyme 2 / transaminase A (ASP4) identical to aspartate
           aminotransferase, cytoplasmic isozyme 2 SP:P46646
           [Arabidopsis thaliana]
          Length = 405

 Score =  137 bits (332), Expect = 2e-32
 Identities = 66/151 (43%), Positives = 90/151 (59%), Gaps = 1/151 (0%)
 Frame = +2

Query: 251 GXPFVLPSVRQAEEILXKK-GLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQNKSNCTVQ 427
           G P VL  VR+AE+ L      +  Y P++G             G+DS  ++     T Q
Sbjct: 44  GKPLVLDVVRRAEQQLANDLSRDKEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQ 103

Query: 428 TLSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFD 607
            LSGTG+LR+G EF+  H  K   I++P PTWGNHP+I  +  L  + +RY+DPK+ G D
Sbjct: 104 CLSGTGSLRVGAEFLATHN-KESVIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLD 162

Query: 608 LQGALEDISKIPEGSIILLHACAHNPTGVDP 700
            +G LED+   P G+I++L ACAHNPTGVDP
Sbjct: 163 FKGMLEDLGAAPPGAIVVLQACAHNPTGVDP 193


>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
           / transaminase A (ASP1) identical to SP|P46643 Aspartate
           aminotransferase, mitochondrial precursor (EC 2.6.1.1)
           (Transaminase A) {Arabidopsis thaliana}
          Length = 430

 Score =  134 bits (325), Expect = 1e-31
 Identities = 73/183 (39%), Positives = 98/183 (53%)
 Frame = +2

Query: 227 GAYXR**XGXPFVLPSVRQAEEILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQN 406
           GAY R   G P VL  VR+AE+ L        Y P+ G            +G++S+ I++
Sbjct: 65  GAY-RDDNGKPVVLECVREAEKRLAGSTFME-YLPMGGSAKMVDLTLKLAYGDNSEFIKD 122

Query: 407 KSNCTVQTLSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFD 586
           K    VQTLSGTGA RL  +F    ++   +I++P PTW NH  I    ++P K Y Y+ 
Sbjct: 123 KRIAAVQTLSGTGACRLFADF-QKRFSPGSQIYIPVPTWSNHHNIWKDAQVPQKTYHYYH 181

Query: 587 PKTNGFDLQGALEDISKIPEGSIILLHACAHNPTGVDPRPQRLGTAV*GSSXRGKLXXFF 766
           P+T G D    ++D+   PEGS  LLHACAHNPTGVDP  ++    +       K   FF
Sbjct: 182 PETKGLDFSALMDDVKNAPEGSFFLLHACAHNPTGVDPTEEQ-WREISQLFKAKKHFAFF 240

Query: 767 DMA 775
           DMA
Sbjct: 241 DMA 243



 Score = 37.1 bits (82), Expect = 0.030
 Identities = 19/51 (37%), Positives = 27/51 (52%)
 Frame = +1

Query: 139 VPMGPPDVILGITEAYKKDTHXNKVNLGVGSIXEMMXXXAICLTISEAGRR 291
           V   P D ILG+TEA+  D    KVN+GVG+  +      +   + EA +R
Sbjct: 36  VEPAPKDPILGVTEAFLADPSPEKVNVGVGAYRDDNGKPVVLECVREAEKR 86


>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
            domain-containing protein / F-box family protein similar
            to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
            pombe}; contains Pfam profiles PF00271: Helicase
            conserved C-terminal domain, PF00176: SNF2 family
            N-terminal domain, PF00646: F-box domain
          Length = 1378

 Score = 30.7 bits (66), Expect = 2.6
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = +2

Query: 395  VIQNKSNCTVQTLSGTGALRLGLEFITNHYAKAKEIW 505
            + QN ++C    + G+GAL L L F+T H    + IW
Sbjct: 1238 MFQNDADCMALLMDGSGALGLDLSFVT-HVFLMEPIW 1273


>At4g18240.1 68417.m02709 starch synthase-related protein contains
            similarity to starch synthase GI:4582783 from [Vigna
            unguiculata]
          Length = 1040

 Score = 29.1 bits (62), Expect = 8.0
 Identities = 21/83 (25%), Positives = 34/83 (40%)
 Frame = +2

Query: 260  FVLPSVRQAEEILXKKGLNHGYPPISGXXXXXXXXXXXXFGEDSQVIQNKSNCTVQTLSG 439
            F++PS+ +   +     + +G  PI+               +D+   Q ++  T QT   
Sbjct: 922  FIIPSIFEPCGLTQMIAMRYGSIPIA-RKTGGLNDSVFDIDDDTIPTQFQNGFTFQTADE 980

Query: 440  TGALRLGLEFITNHYAKAKEIWM 508
             G     LE   NHY K +E WM
Sbjct: 981  QG-FNYALERAFNHYKKDEEKWM 1002


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,142,250
Number of Sequences: 28952
Number of extensions: 418204
Number of successful extensions: 766
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 759
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4019292864
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)