BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_H03_e24_15.seq (1453 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 2.8 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 3.7 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 28 3.7 SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 27 4.9 SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 27 4.9 SPAPB8E5.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 8.6 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 28.3 bits (60), Expect = 2.8 Identities = 18/68 (26%), Positives = 19/68 (27%), Gaps = 1/68 (1%) Frame = +3 Query: 936 PPIPXPP-XPXAPXPSXXKXXXPXTFFFXPXPPPXRRXXXLAPXQRXGPDFPLPGEXKPE 1112 PP+P P P P PS P P P P P P P P Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP 1209 Query: 1113 AXPXXXXP 1136 P P Sbjct: 1210 VPPPSTAP 1217 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.9 bits (59), Expect = 3.7 Identities = 19/54 (35%), Positives = 20/54 (37%), Gaps = 1/54 (1%) Frame = +1 Query: 1066 NGXXPISLCP-GRXSPKRTPPXXXPXXXXSPPXGXPPPFXXXPXAXPRATXPGR 1224 N I L P GR +P PP P PP P A P A PGR Sbjct: 346 NAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPA-IPGR 398 Score = 26.6 bits (56), Expect = 8.6 Identities = 20/75 (26%), Positives = 23/75 (30%) Frame = +1 Query: 1036 PGEXXXSLPXNGXXPISLCPGRXSPKRTPPXXXPXXXXSPPXGXPPPFXXXPXAXPRATX 1215 PG +LP G + P +P PP P S P PP A P Sbjct: 396 PGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPP----SLPPSAPPSLPMGAPAAPPLPP 451 Query: 1216 PGRXXXPTSXGXPXA 1260 P G P A Sbjct: 452 SAPIAPPLPAGMPAA 466 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 27.9 bits (59), Expect = 3.7 Identities = 17/57 (29%), Positives = 20/57 (35%) Frame = +1 Query: 1078 PISLCPGRXSPKRTPPXXXPXXXXSPPXGXPPPFXXXPXAXPRATXPGRXXXPTSXG 1248 PI PG + + P P PP G P PF P PG P + G Sbjct: 507 PIPGAPGMPNLNMSQPPMVPPGMALPP-GMPAPFPGYPAVPAMPGIPGATAPPGAPG 562 >SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 512 Score = 27.5 bits (58), Expect = 4.9 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = -2 Query: 519 LAGMQKYYTMNVFKNYENIMNEKYYKKYYVYFTITDCQITSY 394 LAG +Y+ N K YENI+ K Y YF+ + S+ Sbjct: 391 LAGYPPFYSENPMKLYENILEGKV--NYPSYFSPASIDLLSH 430 >SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual Length = 935 Score = 27.5 bits (58), Expect = 4.9 Identities = 17/46 (36%), Positives = 23/46 (50%) Frame = -1 Query: 373 PFLLLCLVIYRKKRPSAFIFEALKANFN*LLMHIIF*INSFNGFTN 236 PFLL+C+ Y + P + AL L+H IF N+ GF N Sbjct: 493 PFLLICIFFYIQSSPPFYYGYALFPTIFLQLIHSIF-PNTKLGFKN 537 >SPAPB8E5.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 26.6 bits (56), Expect = 8.6 Identities = 10/19 (52%), Positives = 13/19 (68%) Frame = -2 Query: 465 IMNEKYYKKYYVYFTITDC 409 I+NE+ +K Y Y ITDC Sbjct: 32 ILNERAFKHYKTYRRITDC 50 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,744,575 Number of Sequences: 5004 Number of extensions: 63165 Number of successful extensions: 138 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 75 effective length of database: 1,987,178 effective search space used: 810768624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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