BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_H02_e16_16.seq (1445 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 211 2e-55 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 118 2e-27 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 109 9e-25 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 86 1e-17 SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 27 6.4 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 211 bits (515), Expect = 2e-55 Identities = 115/224 (51%), Positives = 130/224 (58%) Frame = +3 Query: 6 HYTEGAELVDSXLXVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 185 HYTEGAEL D+ L VVR+EAE+CD LQGFQ KIREEYPDR+M Sbjct: 105 HYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMM 164 Query: 186 NTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGD 365 T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y D Sbjct: 165 ATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDD 224 Query: 366 LNHLVSLDHVWRYHLSQVPWSVECGSPKLAVNMVPFPRLHFFMPRFRLPXXXXXXXXXXX 545 LNHLVS + P + KLAVNMVPFPRLHFFM F P Sbjct: 225 LNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGF-APLAAIGSSSFQA 283 Query: 546 XPFPSSHSRCSXAKNMMGRXXTRVTGRXLTVGXHXPXSXXMKEV 677 P + A NMM R GR LTV MKEV Sbjct: 284 VSVPELTQQMFDANNMMVAADPR-HGRYLTVAALFRGKVSMKEV 326 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 118 bits (284), Expect = 2e-27 Identities = 66/209 (31%), Positives = 102/209 (48%) Frame = +3 Query: 6 HYTEGAELVDSXLXVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 185 HYT G E++DS L +R+ A++C LQGF ++ EY + Sbjct: 111 HYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSN 170 Query: 186 NTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGD 365 +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY + Sbjct: 171 LQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYEN 230 Query: 366 LNHLVSLDHVWRYHLSQVPWSVECGSPKLAVNMVPFPRLHFFMPRFRLPXXXXXXXXXXX 545 LN L++ + S+ + N+VP+PR+HF + + P Sbjct: 231 LNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYS-PIVSAAKAFHES 289 Query: 546 XPFPSSHSRCSXAKNMMGRXXTRVTGRXL 632 ++C N M + R TGR + Sbjct: 290 NSVQEITNQCFEPYNQMVKCDPR-TGRYM 317 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 109 bits (262), Expect = 9e-25 Identities = 55/161 (34%), Positives = 84/161 (52%) Frame = +3 Query: 6 HYTEGAELVDSXLXVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 185 HYT G ELVD +R+ A++C LQGF ++ EY + Sbjct: 107 HYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSK 166 Query: 186 NTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGD 365 +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y + Sbjct: 167 LQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYEN 226 Query: 366 LNHLVSLDHVWRYHLSQVPWSVECGSPKLAVNMVPFPRLHF 488 LN L++ + S+ + N+VP+PR+HF Sbjct: 227 LNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHF 267 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 85.8 bits (203), Expect = 1e-17 Identities = 46/166 (27%), Positives = 75/166 (45%), Gaps = 1/166 (0%) Frame = +3 Query: 9 YTEGAELVDSXLXVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMN 188 Y+ + + + ++ +EA+ D L+GF ++ + YP +I+ Sbjct: 108 YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQ 167 Query: 189 TYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGD 365 TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I L PT+ Sbjct: 168 TYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQ 227 Query: 366 LNHLVSLDHVWRYHLSQVPWSVECGSPKLAVNMVPFPRLHFFMPRF 503 N LVS + P + + +++P PR HF + + Sbjct: 228 QNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSY 273 >SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr 3|||Manual Length = 577 Score = 27.1 bits (57), Expect = 6.4 Identities = 14/28 (50%), Positives = 15/28 (53%), Gaps = 1/28 (3%) Frame = +1 Query: 79 VYKD-SNSHTPSEAVLAPVWAPFSSLKY 159 VYK S HT S L +W PFS L Y Sbjct: 238 VYKTISPLHTQSTDKLYTIWTPFSELHY 265 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,526,758 Number of Sequences: 5004 Number of extensions: 60768 Number of successful extensions: 159 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 75 effective length of database: 1,987,178 effective search space used: 806794268 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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