BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_G11_e87_13.seq (1470 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0... 262 6e-71 SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ... 261 1e-70 SPAC24C9.10c |mrp4||mitochondrial ribosomal protein subunit S2|S... 42 3e-04 SPAC17C9.13c |cut8||tethering factor for nuclear proteasome Cut8... 29 2.1 SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 29 2.1 SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces... 27 6.5 >SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0A |Schizosaccharomyces pombe|chr 2|||Manual Length = 292 Score = 262 bits (643), Expect = 6e-71 Identities = 121/203 (59%), Positives = 151/203 (74%) Frame = +3 Query: 24 RHXVLALSEEDVTKMLAATTHLGAENVNFQMETYVYKRRGDGTHVINLRRTWEKXXXXXX 203 R +L ++ED+ ++LAA H+G++N+ +M+ YV+KRR DG H++NL +TWEK Sbjct: 6 RPNILNATDEDIKQLLAANCHIGSKNLEVRMDNYVWKRRSDGVHILNLGKTWEKLVLAAR 65 Query: 204 XXXXXEDPADVFVISSRAFGQRAVLKFAAHTGATPIAGRFTPGAFTNQIQAAFREPRLLI 383 E+PADV V+S+R +G RAVLKFAAHTGAT IAGRFTPG FTN I +REPRL++ Sbjct: 66 VIATIENPADVCVVSTRTYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLIV 125 Query: 384 VLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVL 563 V DP D Q I EAS+VNIPVIALC+TDS L VDIAIP N K SIGL+W+LLAREVL Sbjct: 126 VTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDIAIPTNNKGRKSIGLIWYLLAREVL 185 Query: 564 RLRGVLSRDQRWDVVVDLFFYRD 632 R+RG LSR WDV+ DL+FYRD Sbjct: 186 RVRGTLSRSAPWDVMPDLYFYRD 208 >SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein S0B|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 261 bits (640), Expect = 1e-70 Identities = 124/203 (61%), Positives = 150/203 (73%) Frame = +3 Query: 24 RHXVLALSEEDVTKMLAATTHLGAENVNFQMETYVYKRRGDGTHVINLRRTWEKXXXXXX 203 R VL +++D+ +LAA +H+G++N+ +ME YV+KRR DG H+INL +TWEK Sbjct: 7 RPSVLNATDDDIKNLLAADSHIGSKNLEVRMENYVWKRRSDGIHIINLGKTWEKLVLAAR 66 Query: 204 XXXXXEDPADVFVISSRAFGQRAVLKFAAHTGATPIAGRFTPGAFTNQIQAAFREPRLLI 383 E+PADV VISSR +G RAVLKFAAHTGAT IAGRFTPG FTN I +REPRL+I Sbjct: 67 VIATIENPADVCVISSRPYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLII 126 Query: 384 VLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVL 563 V DP D Q I EAS+VNIPVIALC+TDS L VD+AIP N K SIGL W+LLAREVL Sbjct: 127 VTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDVAIPINNKGYKSIGLAWYLLAREVL 186 Query: 564 RLRGVLSRDQRWDVVVDLFFYRD 632 RLRG +SR W+V+ DL+FYRD Sbjct: 187 RLRGNISRTTAWEVMPDLYFYRD 209 >SPAC24C9.10c |mrp4||mitochondrial ribosomal protein subunit S2|Schizosaccharomyces pombe|chr 1|||Manual Length = 263 Score = 41.5 bits (93), Expect = 3e-04 Identities = 20/62 (32%), Positives = 33/62 (53%) Frame = +3 Query: 369 PRLLIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLL 548 P L+++L+P ++ EA ++P I + +TD+ R V IP N S L+ LL Sbjct: 180 PDLMVILNPLENKSACLEAQKTHVPTIGIIDTDADPRMVTYPIPANDDSLRCTDLIAGLL 239 Query: 549 AR 554 +R Sbjct: 240 SR 241 >SPAC17C9.13c |cut8||tethering factor for nuclear proteasome Cut8|Schizosaccharomyces pombe|chr 1|||Manual Length = 262 Score = 28.7 bits (61), Expect = 2.1 Identities = 16/61 (26%), Positives = 33/61 (54%) Frame = -1 Query: 774 PVLHLQPMMQLVQHSQSIPHELAAALPLWERRQPVP*LVALPSHSLQGHDRRTSQRQHPN 595 PV+ L P+ +L +H+++ + + AL ++ Q LV SH+ Q + + + +H N Sbjct: 184 PVVPLLPLEELEEHNKTSQNRMEEALNYLKQLQKNEPLVHERSHTFQQTNPQNNFHRHTN 243 Query: 594 A 592 + Sbjct: 244 S 244 >SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual Length = 539 Score = 28.7 bits (61), Expect = 2.1 Identities = 23/84 (27%), Positives = 38/84 (45%), Gaps = 5/84 (5%) Frame = +3 Query: 291 HTGATPIAGRFTPGAFTNQIQAAFR--EPRLLIVLDPAQDHQPI---TEASYVNIPVIAL 455 + A P G T+ I A F+ +P + ++ D + T A + + L Sbjct: 29 YVNAAPHLGHLYSLVLTDAI-ARFQNLKPDVSVISSTGTDEHGLKVQTVAQTEGVSPLQL 87 Query: 456 CNTDSPLRFVDIAIPCNTKSSHSI 527 C+ +S RF D+A+ NTK +H I Sbjct: 88 CDRNSK-RFADLAVAANTKFTHFI 110 >SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 3|||Manual Length = 561 Score = 27.1 bits (57), Expect = 6.5 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = +3 Query: 525 IGLMWWLLAREVLRLRGVLSRDQRWD 602 IGL W L REV R + + +R++ WD Sbjct: 365 IGLKWTLKLREVERKQLLTAREKWWD 390 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,382,028 Number of Sequences: 5004 Number of extensions: 74613 Number of successful extensions: 180 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 177 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 180 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 818637862 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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