SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_G11_e87_13.seq
         (1470 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0...   262   6e-71
SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ...   261   1e-70
SPAC24C9.10c |mrp4||mitochondrial ribosomal protein subunit S2|S...    42   3e-04
SPAC17C9.13c |cut8||tethering factor for nuclear proteasome Cut8...    29   2.1  
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc...    29   2.1  
SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces...    27   6.5  

>SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0A
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 292

 Score =  262 bits (643), Expect = 6e-71
 Identities = 121/203 (59%), Positives = 151/203 (74%)
 Frame = +3

Query: 24  RHXVLALSEEDVTKMLAATTHLGAENVNFQMETYVYKRRGDGTHVINLRRTWEKXXXXXX 203
           R  +L  ++ED+ ++LAA  H+G++N+  +M+ YV+KRR DG H++NL +TWEK      
Sbjct: 6   RPNILNATDEDIKQLLAANCHIGSKNLEVRMDNYVWKRRSDGVHILNLGKTWEKLVLAAR 65

Query: 204 XXXXXEDPADVFVISSRAFGQRAVLKFAAHTGATPIAGRFTPGAFTNQIQAAFREPRLLI 383
                E+PADV V+S+R +G RAVLKFAAHTGAT IAGRFTPG FTN I   +REPRL++
Sbjct: 66  VIATIENPADVCVVSTRTYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLIV 125

Query: 384 VLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVL 563
           V DP  D Q I EAS+VNIPVIALC+TDS L  VDIAIP N K   SIGL+W+LLAREVL
Sbjct: 126 VTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDIAIPTNNKGRKSIGLIWYLLAREVL 185

Query: 564 RLRGVLSRDQRWDVVVDLFFYRD 632
           R+RG LSR   WDV+ DL+FYRD
Sbjct: 186 RVRGTLSRSAPWDVMPDLYFYRD 208


>SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein
           S0B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 287

 Score =  261 bits (640), Expect = 1e-70
 Identities = 124/203 (61%), Positives = 150/203 (73%)
 Frame = +3

Query: 24  RHXVLALSEEDVTKMLAATTHLGAENVNFQMETYVYKRRGDGTHVINLRRTWEKXXXXXX 203
           R  VL  +++D+  +LAA +H+G++N+  +ME YV+KRR DG H+INL +TWEK      
Sbjct: 7   RPSVLNATDDDIKNLLAADSHIGSKNLEVRMENYVWKRRSDGIHIINLGKTWEKLVLAAR 66

Query: 204 XXXXXEDPADVFVISSRAFGQRAVLKFAAHTGATPIAGRFTPGAFTNQIQAAFREPRLLI 383
                E+PADV VISSR +G RAVLKFAAHTGAT IAGRFTPG FTN I   +REPRL+I
Sbjct: 67  VIATIENPADVCVISSRPYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLII 126

Query: 384 VLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVL 563
           V DP  D Q I EAS+VNIPVIALC+TDS L  VD+AIP N K   SIGL W+LLAREVL
Sbjct: 127 VTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDVAIPINNKGYKSIGLAWYLLAREVL 186

Query: 564 RLRGVLSRDQRWDVVVDLFFYRD 632
           RLRG +SR   W+V+ DL+FYRD
Sbjct: 187 RLRGNISRTTAWEVMPDLYFYRD 209


>SPAC24C9.10c |mrp4||mitochondrial ribosomal protein subunit
           S2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 263

 Score = 41.5 bits (93), Expect = 3e-04
 Identities = 20/62 (32%), Positives = 33/62 (53%)
 Frame = +3

Query: 369 PRLLIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLL 548
           P L+++L+P ++     EA   ++P I + +TD+  R V   IP N  S     L+  LL
Sbjct: 180 PDLMVILNPLENKSACLEAQKTHVPTIGIIDTDADPRMVTYPIPANDDSLRCTDLIAGLL 239

Query: 549 AR 554
           +R
Sbjct: 240 SR 241


>SPAC17C9.13c |cut8||tethering factor for nuclear proteasome
           Cut8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 262

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 16/61 (26%), Positives = 33/61 (54%)
 Frame = -1

Query: 774 PVLHLQPMMQLVQHSQSIPHELAAALPLWERRQPVP*LVALPSHSLQGHDRRTSQRQHPN 595
           PV+ L P+ +L +H+++  + +  AL   ++ Q    LV   SH+ Q  + + +  +H N
Sbjct: 184 PVVPLLPLEELEEHNKTSQNRMEEALNYLKQLQKNEPLVHERSHTFQQTNPQNNFHRHTN 243

Query: 594 A 592
           +
Sbjct: 244 S 244


>SPAC27E2.06c |||methionine-tRNA ligase,
           mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 539

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 23/84 (27%), Positives = 38/84 (45%), Gaps = 5/84 (5%)
 Frame = +3

Query: 291 HTGATPIAGRFTPGAFTNQIQAAFR--EPRLLIVLDPAQDHQPI---TEASYVNIPVIAL 455
           +  A P  G       T+ I A F+  +P + ++     D   +   T A    +  + L
Sbjct: 29  YVNAAPHLGHLYSLVLTDAI-ARFQNLKPDVSVISSTGTDEHGLKVQTVAQTEGVSPLQL 87

Query: 456 CNTDSPLRFVDIAIPCNTKSSHSI 527
           C+ +S  RF D+A+  NTK +H I
Sbjct: 88  CDRNSK-RFADLAVAANTKFTHFI 110


>SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 561

 Score = 27.1 bits (57), Expect = 6.5
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = +3

Query: 525 IGLMWWLLAREVLRLRGVLSRDQRWD 602
           IGL W L  REV R + + +R++ WD
Sbjct: 365 IGLKWTLKLREVERKQLLTAREKWWD 390


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,382,028
Number of Sequences: 5004
Number of extensions: 74613
Number of successful extensions: 180
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 818637862
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -