BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_G10_e79_14.seq (1502 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit S... 31 0.41 SPBC365.09c |||human KIN homolog|Schizosaccharomyces pombe|chr 2... 30 0.72 SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces po... 30 0.95 SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 27 5.1 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 27 8.9 SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosacch... 27 8.9 SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit |Sch... 27 8.9 >SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit S28|Schizosaccharomyces pombe|chr 2|||Manual Length = 288 Score = 31.1 bits (67), Expect = 0.41 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = +2 Query: 398 DNLEKIRNSATENTDDFESIATNTEQLPTGRNQVKSNSD 514 + EKIR SATE T DF + + P+ ++SN D Sbjct: 116 EKFEKIRASATEETKDFIKL-NEVNEFPSSDTSLESNQD 153 >SPBC365.09c |||human KIN homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 304 Score = 30.3 bits (65), Expect = 0.72 Identities = 20/82 (24%), Positives = 36/82 (43%), Gaps = 1/82 (1%) Frame = +2 Query: 362 FFSFINKQHLND-DNLEKIRNSATENTDDFESIATNTEQLPTGRNQVKSNSDTHDGDIVK 538 F S+I+K N N + E +D+ + + EQ+ ++N D DG Sbjct: 138 FISYIDKNPANILRNEANKKRERQEKSDEEQRLRLLDEQIKRAYESAQNNEDNKDGS--S 195 Query: 539 KETPVSRELATQKRHIEAQVHI 604 +E PV E+ K+ Q+++ Sbjct: 196 REQPVLHEIDLSKKGNPIQLNL 217 >SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 451 Score = 29.9 bits (64), Expect = 0.95 Identities = 22/108 (20%), Positives = 46/108 (42%), Gaps = 6/108 (5%) Frame = +2 Query: 302 FSQPATLAIYD-PPSEY-----TYEIFFSFINKQHLNDDNLEKIRNSATENTDDFESIAT 463 FS P T I D ++Y T E+ F+ K + N + + NT + T Sbjct: 322 FSNPHTKHIMDLVRTQYRNVLRTRELIPEFLEKIRSSYSNNQNFDSQNAFNTSAAGTAGT 381 Query: 464 NTEQLPTGRNQVKSNSDTHDGDIVKKETPVSRELATQKRHIEAQVHIP 607 E + G+N + ++++T D + ++ ++ + + +H+P Sbjct: 382 REETISNGQNGIVASAETSKKDDGVQSASLNASMSARSHARQRSIHVP 429 >SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 518 Score = 27.5 bits (58), Expect = 5.1 Identities = 16/50 (32%), Positives = 25/50 (50%) Frame = -1 Query: 191 INKVNKYYSKMFYHCV*GTVKVLSSNRTTVARVPWTLVPNXLQXRGXPLV 42 + K+ ++ SKM HC GTV + N + + RV + N + G LV Sbjct: 152 LRKILEHVSKM--HCQLGTVAIAGLNSSNIQRVIYLSEANGKRIDGIALV 199 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 26.6 bits (56), Expect = 8.9 Identities = 21/86 (24%), Positives = 41/86 (47%), Gaps = 6/86 (6%) Frame = +2 Query: 410 KIRNSATENTDDFESIATNTEQLPTGRNQVKSN-SDTHDGDIVKKETPVSRELA---TQK 577 K + + T++ + +TN+ LPT V++N S +H+ T + L+ T+ Sbjct: 41 KAKTPSNHATEELQQSSTNSTTLPTQEAAVETNASASHETSFALPTTSPAASLSISPTKS 100 Query: 578 RHIEAQVHIPAVV--AKSTGAIPKDN 649 + ++ ++ A V ST A P+ N Sbjct: 101 AAVSSEPNVEADVKSLSSTPAAPQLN 126 >SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 26.6 bits (56), Expect = 8.9 Identities = 31/136 (22%), Positives = 59/136 (43%), Gaps = 12/136 (8%) Frame = +2 Query: 212 KKETERVRSQSVPGHSPEH--NEHHLGYNNRVFSQPATLAIYDPPSEYTYEIFFSFINKQ 385 K+ R++ + SP H N H N+R Q T ++ E+ NK+ Sbjct: 58 KRRKRRLKGSNSDSDSPHHASNPHP---NSRQKHQHQTSKSVPRSQRFSRELNVKKENKK 114 Query: 386 HLNDDNLEKI--RNSATENTDDF-----ESIATNTEQLPTGRNQVKSNSDTHD--GDIVK 538 + ++ + S T+D +S+ TN+++L RN+VK S + + +K Sbjct: 115 VFSSQTTKRQSRKQSTALTTNDTSIILDDSLHTNSKKLGKTRNEVKEESQKRELVSNSIK 174 Query: 539 KET-PVSRELATQKRH 583 + T P + + T+ R+ Sbjct: 175 EATSPKTSSILTKPRN 190 >SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 26.6 bits (56), Expect = 8.9 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = -2 Query: 616 NYSWYMNLGFNVTFLCCKFPGHRSFFLYY 530 NYSW+M + + + KF R F LY+ Sbjct: 115 NYSWFMPVFHSYPYNIQKFDAARYFILYH 143 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,639,106 Number of Sequences: 5004 Number of extensions: 85049 Number of successful extensions: 270 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 257 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 270 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 840441776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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