BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_G08_e63_14.seq (1537 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 81 5e-16 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 54 4e-08 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 54 4e-08 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 54 4e-08 SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||... 27 5.2 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 80.6 bits (190), Expect = 5e-16 Identities = 42/104 (40%), Positives = 58/104 (55%) Frame = +2 Query: 218 FXAXVVILEXXSIICAGYSDVMHIHCAAEEXTVKALICLVDXKXGDKSKTXPRXX*HDLV 397 F A + ILE SI+ GYS VMHIH A EE + L+ +D K KSK P + Sbjct: 560 FIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMK 618 Query: 398 AIMRIECAGVICLEPFXXXXXMGXFTXRDENXTIAIGKVLKVIE 529 I +E +C+E F MG FT RD+ T+A+GKV+K+++ Sbjct: 619 IIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 54.4 bits (125), Expect = 4e-08 Identities = 33/104 (31%), Positives = 49/104 (47%) Frame = +2 Query: 218 FXAXVVILEXXSIICAGYSDVMHIHCAAEEXTVKALICLVDXKXGDKSKTXPRXX*HDLV 397 F A V+IL I AGYS V+ H A LI +D + G K + P+ Sbjct: 338 FTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDA 397 Query: 398 AIMRIECAGVICLEPFXXXXXMGXFTXRDENXTIAIGKVLKVIE 529 I ++ + +C+E F +G F RD T+A+G V+K +E Sbjct: 398 CIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVG-VIKAVE 440 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 54.4 bits (125), Expect = 4e-08 Identities = 33/104 (31%), Positives = 49/104 (47%) Frame = +2 Query: 218 FXAXVVILEXXSIICAGYSDVMHIHCAAEEXTVKALICLVDXKXGDKSKTXPRXX*HDLV 397 F A V+IL I AGYS V+ H A LI +D + G K + P+ Sbjct: 338 FTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDA 397 Query: 398 AIMRIECAGVICLEPFXXXXXMGXFTXRDENXTIAIGKVLKVIE 529 I ++ + +C+E F +G F RD T+A+G V+K +E Sbjct: 398 CIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVG-VIKAVE 440 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 54.4 bits (125), Expect = 4e-08 Identities = 33/104 (31%), Positives = 49/104 (47%) Frame = +2 Query: 218 FXAXVVILEXXSIICAGYSDVMHIHCAAEEXTVKALICLVDXKXGDKSKTXPRXX*HDLV 397 F A V+IL I AGYS V+ H A LI +D + G K + P+ Sbjct: 338 FTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDA 397 Query: 398 AIMRIECAGVICLEPFXXXXXMGXFTXRDENXTIAIGKVLKVIE 529 I ++ + +C+E F +G F RD T+A+G V+K +E Sbjct: 398 CIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVG-VIKAVE 440 >SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2|||Manual Length = 525 Score = 27.5 bits (58), Expect = 5.2 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -1 Query: 295 AMYVHDVGVSRAYYRXXLEDHYXRXE 218 A YVHD+ V++ YY L D + E Sbjct: 85 AAYVHDLHVAQRYYADRLSDRVKKSE 110 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,023,214 Number of Sequences: 5004 Number of extensions: 35770 Number of successful extensions: 46 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 44 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 45 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 862245690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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