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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_G08_e63_14.seq
         (1537 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,...    65   1e-10
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E...    61   2e-09

>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
           putative similar to EF-1-alpha-related GTP-binding
           protein gi|1009232|gb|AAA79032
          Length = 532

 Score = 64.9 bits (151), Expect = 1e-10
 Identities = 38/113 (33%), Positives = 53/113 (46%), Gaps = 2/113 (1%)
 Frame = +2

Query: 194 NLSPLVRXFXAXVVILEXX--SIICAGYSDVMHIHCAAEEXTVKALICLVDXKXGDKSKT 367
           N  P V  F A + ILE    +I  AGY  ++HIH   EE  +  L   +D K     K 
Sbjct: 414 NPVPAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDLKTRKPMKK 473

Query: 368 XPRXX*HDLVAIMRIECAGVICLEPFXXXXXMGXFTXRDENXTIAIGKVLKVI 526
                 +    + RI+    IC+E F     +G FT R E  TIA+GKV +++
Sbjct: 474 KVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKVTELL 526


>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
           EF-1-alpha, putative contains similarity to
           SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
           [Aeropyrum pernix]
          Length = 667

 Score = 61.3 bits (142), Expect = 2e-09
 Identities = 30/100 (30%), Positives = 49/100 (49%)
 Frame = +2

Query: 230 VVILEXXSIICAGYSDVMHIHCAAEEXTVKALICLVDXKXGDKSKTXPRXX*HDLVAIMR 409
           V++LE  + I  G     H+H A E  TV  L+ ++D K G  +K  PR       A++ 
Sbjct: 566 VLVLEGATPILLGSQLEFHVHHAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLE 625

Query: 410 IECAGVICLEPFXXXXXMGXFTXRDENXTIAIGKVLKVIE 529
           +     +C+E F     +G    R    T+A+GKV ++I+
Sbjct: 626 VSLQNPVCVETFSESRALGRVFLRSSGRTVAMGKVTRIIQ 665


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,703,381
Number of Sequences: 28952
Number of extensions: 187585
Number of successful extensions: 181
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4115678784
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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