Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_G07_e55_13.seq
(1514 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 31 0.32
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 30 0.73
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 29 1.7
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 29 1.7
SPAC1F3.01 |rrp6|SPAC3H8.11|exosome subunit Rrp6 |Schizosaccharo... 28 2.9
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 2.9
SPCC1494.06c |||ATP-dependent RNA helicase Dbp9 |Schizosaccharom... 28 3.9
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 28 3.9
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 27 5.1
SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 27 6.8
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 31.5 bits (68), Expect = 0.32
Identities = 25/107 (23%), Positives = 49/107 (45%)
Frame = +1
Query: 148 RFARAKGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEH 327
R A K + + EKA + + +E KAK+ ++ KRE EK + E EN E
Sbjct: 611 REAEEKAKREAEEKAKREAEE-KAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREA 669
Query: 328 KIQKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKE 468
+ +K K + +++ + + + ++K + K+E + + E
Sbjct: 670 E-EKAKRE-AEENAKREAEEKVKRETEENAKRKAEEEGKREADKNPE 714
Score = 27.5 bits (58), Expect = 5.1
Identities = 24/117 (20%), Positives = 46/117 (39%), Gaps = 2/117 (1%)
Frame = +1
Query: 127 KVSVRMTRFARAKGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENY 306
K R A K + E A + + +E KAK+ ++ KRE EK + E
Sbjct: 540 KAEAEAKRKAEEKARLEAEENAKREAEE-QAKREAEEKAKREAEEKAKREAEEKAKREAE 598
Query: 307 ENFL--XEHKIQKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQ 471
EN E K ++ EK++ + ++K + +++ + + E+
Sbjct: 599 ENAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEE 655
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 30.3 bits (65), Expect = 0.73
Identities = 23/90 (25%), Positives = 42/90 (46%), Gaps = 1/90 (1%)
Frame = +1
Query: 223 KEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEHKIQKXKATWCDFPE-SEKSQNNLTK 399
K++L K+ Q + +++ + + + + K K T D PE S S + +
Sbjct: 150 KDELEKSIQRCSELEEKINSLESAQSIEQEVISSLKDDKTVETKNDVPEVSRPSTDTIGV 209
Query: 400 SVDMTLQKKKVKHDKQELEIDKEQIEITTE 489
S ++ +KKK Q+ + K+ IE TTE
Sbjct: 210 SSALSKKKKKRNRKNQKKKSTKQNIEATTE 239
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 29.1 bits (62), Expect = 1.7
Identities = 22/69 (31%), Positives = 32/69 (46%), Gaps = 5/69 (7%)
Frame = +1
Query: 301 NYENFL---XEHKIQKXKATWCDFPESEKSQN--NLTKSVDMTLQKKKVKHDKQELEIDK 465
NY+ FL E +Q K + K QN N+T S K KVK ++LE DK
Sbjct: 503 NYDTFLKVRAERDVQLAKKARQQEKDMAKLQNKLNMTGSEQQKKAKAKVKAMNKKLEKDK 562
Query: 466 EQIEITTEK 492
+ ++ E+
Sbjct: 563 QSGKVLDEE 571
>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 489
Score = 29.1 bits (62), Expect = 1.7
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +1
Query: 328 KIQKXKATWCDFPESEKSQNNLTKSV-DMTLQKKKVKHDKQELEIDKEQIEITTEKP 495
KIQK KA E E + L K++ D+ K+ + E DK+QIE+ P
Sbjct: 387 KIQKLKAAKLP-SEEENTTKWLNKAINDLVSSLSKINFSTEGTEFDKKQIEMIQNLP 442
>SPAC1F3.01 |rrp6|SPAC3H8.11|exosome subunit Rrp6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 777
Score = 28.3 bits (60), Expect = 2.9
Identities = 21/89 (23%), Positives = 37/89 (41%)
Frame = +1
Query: 163 KGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEHKIQKX 342
+ S N K P + P E E + ++ T +KKR++ +K++ EH +
Sbjct: 657 RDSSKLNHKEPSN--PIEERNEDIEPSEASTSVSKKRKQKKKKKNSGKLTIEAEH-VSND 713
Query: 343 KATWCDFPESEKSQNNLTKSVDMTLQKKK 429
+ P K+Q N +D + K K
Sbjct: 714 SPIINEAPFDYKNQKNFIADLDSDVGKNK 742
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.3 bits (60), Expect = 2.9
Identities = 21/108 (19%), Positives = 44/108 (40%), Gaps = 2/108 (1%)
Frame = +1
Query: 286 KQRIENYENFLXEH--KIQKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEI 459
K+RI N + + K++ C++ +K +N+ K+++ + + + E
Sbjct: 1183 KERIRNIKKLKTDMLTKLENTVKYLCEYDSIDKVRNSFFKNLNSFYEMYSIAKAENEERF 1242
Query: 460 DKEQIEITTEKPEXXXXXXXXXXXXXXXXXMQEVQTDSKQTDEQDKLS 603
+KE+ I +E + +E+ DS D+QD S
Sbjct: 1243 EKEKRRIMSEDRDKLIRGRQKTSIVAKYRNKRELPEDS--DDKQDTAS 1288
>SPCC1494.06c |||ATP-dependent RNA helicase Dbp9
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 595
Score = 27.9 bits (59), Expect = 3.9
Identities = 20/69 (28%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
Frame = +1
Query: 253 TDDAKKREEAEKQRIENYENFLXEHKIQKXKATWCDFPESEKSQNN----LTKSVDMTLQ 420
TD++ E E+ +EN +F+ E I + T + E + N L + D + +
Sbjct: 317 TDESGMMGEIEE--LENNVDFVEEEVISTDQPTLDKMKDQENADVNDESILAAAKDKSKK 374
Query: 421 KKKVKHDKQ 447
KK+VK DK+
Sbjct: 375 KKRVKQDKE 383
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 27.9 bits (59), Expect = 3.9
Identities = 19/77 (24%), Positives = 35/77 (45%)
Frame = +1
Query: 169 SKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEHKIQKXKA 348
+K + AP TP + + +++A++ + D +++ KQ N ++ A
Sbjct: 167 AKVAPAPAPSTKTPLANISKPVVQAQKDSKD-NSWDDSSKQSNTQTANTTSNLRVP-VNA 224
Query: 349 TWCDFPESEKSQNNLTK 399
+W D EKSQ N K
Sbjct: 225 SWSDAGRKEKSQENKPK 241
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 27.5 bits (58), Expect = 5.1
Identities = 19/71 (26%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = +1
Query: 121 FSKVSVRMTRFARAKGSKASNEKAPE-DGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRI 297
FS++S + KGSKA +E AP+ P + + K+ + KK K R+
Sbjct: 433 FSRLSFSRSSSRMKKGSKAKHEDAPDVPAIPHAYIADSSTKSSY--RNGKKTPTRTKSRM 490
Query: 298 ENYENFLXEHK 330
+ + N+ K
Sbjct: 491 QQFINWFKPSK 501
>SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 161
Score = 27.1 bits (57), Expect = 6.8
Identities = 28/105 (26%), Positives = 49/105 (46%), Gaps = 14/105 (13%)
Frame = +1
Query: 220 MKEQLLKAKQFTDDAKKREEAEKQRIENYENFLX------EHKIQKXKATWCDFPESEKS 381
++E++ A+ TD+A R EA + +++ E L E +K +A E E+
Sbjct: 4 LREKINAARAETDEAVARAEAAEAKLKEVELQLSLKEQEYESLSRKSEAAESQLEELEEE 63
Query: 382 QNNLTKSVDMT-LQK-------KKVKHDKQELEIDKEQIEITTEK 492
L D +QK +KV+ ++ELE + + + TTEK
Sbjct: 64 TKQLRLKADNEDIQKTEAEQLSRKVELLEEELETNDKLLRETTEK 108
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,519,503
Number of Sequences: 5004
Number of extensions: 51254
Number of successful extensions: 149
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 848370472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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