Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_G07_e55_13.seq
(1514 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 35 0.16
At5g54410.1 68418.m06777 hypothetical protein 34 0.21
At3g28770.1 68416.m03591 expressed protein 33 0.49
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 33 0.65
At5g16730.1 68418.m01959 expressed protein weak similarity to mi... 32 0.86
At5g55860.1 68418.m06963 expressed protein contains Pfam profile... 32 1.1
At5g42540.1 68418.m05178 5'-3' exoribonuclease (XRN2) identical ... 31 1.5
At3g49050.1 68416.m05358 lipase class 3 family protein / calmodu... 30 3.5
At3g30420.1 68416.m03847 hypothetical protein low similarity to ... 30 3.5
At4g23800.1 68417.m03422 high mobility group (HMG1/2) family pro... 30 4.6
At1g56210.1 68414.m06460 copper chaperone (CCH)-related low simi... 30 4.6
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 30 4.6
At3g13225.1 68416.m01660 WW domain-containing protein contains P... 29 8.0
At2g24440.1 68415.m02921 expressed protein 29 8.0
>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
Nicotiana tabacum, EMBL:AB009883
Length = 1008
Score = 34.7 bits (76), Expect = 0.16
Identities = 26/120 (21%), Positives = 59/120 (49%), Gaps = 2/120 (1%)
Frame = +1
Query: 139 RMTRFARAKGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQ-RIENYENF 315
+M + + K S++ E + T E + + K+ ++KK+ + E++ + E E+
Sbjct: 727 KMKKERKRKKSESKKEGGEGEETQKEANESTKKERKRKKSESKKQSDGEEETQKEPSEST 786
Query: 316 LXEHKIQKXKATW-CDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQIEITTEK 492
E K + ++ + E E+++ +S ++KK KHD++E+ + E+ E +K
Sbjct: 787 KKERKRKNPESKKKAEAVEEEETRKESVESTKKERKRKKPKHDEEEVPNETEKPEKKKKK 846
>At5g54410.1 68418.m06777 hypothetical protein
Length = 219
Score = 34.3 bits (75), Expect = 0.21
Identities = 28/99 (28%), Positives = 46/99 (46%), Gaps = 1/99 (1%)
Frame = +1
Query: 172 KASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEHKIQK-XKA 348
+ NEK + + +K+ K K ++ K+ + EK R E E L E K + +
Sbjct: 69 RLENEKRKQALKDAKDLKDLTYKTK--VENKLKKTQPEKDRAEEEEKDLTEEKKKDPTEE 126
Query: 349 TWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDK 465
D E +K + K D T +KKK +++ELEI +
Sbjct: 127 EEKDPTEEKKKEPAEEKKKDPTEEKKKDPAEEEELEIKR 165
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 33.1 bits (72), Expect = 0.49
Identities = 22/122 (18%), Positives = 53/122 (43%)
Frame = +1
Query: 133 SVRMTRFARAKGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYEN 312
SV+ + + +G+K N+ + + ++ K + + KK+EE +K+ + N
Sbjct: 913 SVKYKKDEKKEGNKEENKDTINTSSKQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNELK 972
Query: 313 FLXEHKIQKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQIEITTEK 492
++K + K+ E K +S D + ++ K +++ KE+ + +K
Sbjct: 973 KQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEYEEKKSKTKEEAKKEKKK 1032
Query: 493 PE 498
+
Sbjct: 1033 SQ 1034
Score = 29.9 bits (64), Expect = 4.6
Identities = 28/109 (25%), Positives = 50/109 (45%)
Frame = +1
Query: 145 TRFARAKGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXE 324
T+ K K S +K E+ E K + K + AKK+EE K++ E+ EN +
Sbjct: 1022 TKEEAKKEKKKSQDKKREEKDS-EERKSKKEKEESRDLKAKKKEEETKEKKES-ENHKSK 1079
Query: 325 HKIQKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQ 471
K K + D +K ++ K + +K + DK+++E ++Q
Sbjct: 1080 KKEDKKEHE--DNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQ 1126
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 32.7 bits (71), Expect = 0.65
Identities = 21/76 (27%), Positives = 39/76 (51%)
Frame = +1
Query: 244 KQFTDDAKKREEAEKQRIENYENFLXEHKIQKXKATWCDFPESEKSQNNLTKSVDMTLQK 423
K+ + D K +AEK+R++ E L + + +K KA + S+ + S D +
Sbjct: 150 KKVSKDKKAMRKAEKERLKEGE--LADEEQRKLKAKKKALSNGKDSKTSKNHSSDEDISP 207
Query: 424 KKVKHDKQELEIDKEQ 471
K HD+ LE+D+++
Sbjct: 208 K---HDENALEVDEDE 220
>At5g16730.1 68418.m01959 expressed protein weak similarity to
microtubule binding protein D-CLIP-190 [Drosophila
melanogaster] GI:2773363, SMC2-like condensin
[Arabidopsis thaliana] GI:14279543
Length = 853
Score = 32.3 bits (70), Expect = 0.86
Identities = 31/154 (20%), Positives = 66/154 (42%), Gaps = 2/154 (1%)
Frame = +1
Query: 163 KGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLX--EHKIQ 336
K + SN+K + T +KE+++ + K+ E +QR+ + E + E +++
Sbjct: 352 KQLEGSNDKLHDTETEITDLKERIVTLETTVAKQKEDLEVSEQRLGSVEEEVSKNEKEVE 411
Query: 337 KXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQIEITTEKPEXXXXXX 516
K K+ + + EK++ L K D T + +++ +K +L D E + EK +
Sbjct: 412 KLKSE-LETVKEEKNR-ALKKEQDATSRVQRLSEEKSKLLSDLESSKEEEEKSKKAMESL 469
Query: 517 XXXXXXXXXXXMQEVQTDSKQTDEQDKLSVNXLK 618
+ + Q D + + ++ LK
Sbjct: 470 ASALHEVSSEGRELKEKLLSQGDHEYETQIDDLK 503
>At5g55860.1 68418.m06963 expressed protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827);
expression supported by MPSS
Length = 649
Score = 31.9 bits (69), Expect = 1.1
Identities = 33/108 (30%), Positives = 51/108 (47%), Gaps = 2/108 (1%)
Frame = +1
Query: 160 AKGSKA-SNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKR-EEAEKQRIENYENFLXEHKI 333
AK S A NE PE EV QL + D+ +K+ E A+ I++ E +
Sbjct: 254 AKKSLALKNEFDPEFAKKLEV---QLTETYNEIDELQKQMETAKASDIDSVNGVSLE--L 308
Query: 334 QKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQIE 477
+ K + E EKS L +S+ L+ K++HD E+E + +IE
Sbjct: 309 NEAKGLFEKLVEEEKSLQELVESLKAELKNVKMEHD--EVEAKEAEIE 354
>At5g42540.1 68418.m05178 5'-3' exoribonuclease (XRN2) identical to
XRN2 [Arabidopsis thaliana] GI:11875630; contains Pfam
domain PF03159: Putative 5'-3' exonuclease domain
Length = 1012
Score = 31.5 bits (68), Expect = 1.5
Identities = 23/82 (28%), Positives = 42/82 (51%)
Frame = +1
Query: 193 PEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEHKIQKXKATWCDFPES 372
P TP ++ Q KA + + + RE+++K + N L K++K K+ +F
Sbjct: 901 PSQITPKKMNSPQRQKAWKKDETPQSREKSKKLKSSLKVNPL---KMKKTKSPQREFTR- 956
Query: 373 EKSQNNLTKSVDMTLQKKKVKH 438
EK + N+T +T +++VKH
Sbjct: 957 EKKKENITPQRKLTKAQRQVKH 978
>At3g49050.1 68416.m05358 lipase class 3 family protein /
calmodulin-binding heat-shock protein, putative
calmodulin-binding heat-shock protein, Nicotiana
tabacum, PIR:T04107
Length = 477
Score = 30.3 bits (65), Expect = 3.5
Identities = 19/76 (25%), Positives = 38/76 (50%), Gaps = 7/76 (9%)
Frame = +1
Query: 256 DDAKKREEAEKQRIENYENFLXEHKIQ----KXKATWCDFPESEK---SQNNLTKSVDMT 414
++ KK E EKQR+E E+ EH ++ +A D P +E K+ +
Sbjct: 348 ENEKKMEIPEKQRMERQESLAREHNLEYRAALRRAVTLDVPHAESMAYEYGTFDKTQEDE 407
Query: 415 LQKKKVKHDKQELEID 462
++++V+ +++E + D
Sbjct: 408 TEEEEVETEEEEEDTD 423
>At3g30420.1 68416.m03847 hypothetical protein low similarity to
SP|Q9UUA2 DNA repair and recombination protein pif1,
mitochondrial precursor {Schizosaccharomyces pombe}
Length = 837
Score = 30.3 bits (65), Expect = 3.5
Identities = 19/87 (21%), Positives = 46/87 (52%)
Frame = +1
Query: 211 WEVMKEQLLKAKQFTDDAKKREEAEKQRIENYENFLXEHKIQKXKATWCDFPESEKSQNN 390
WE M + +L+ +Q + + E K+ +E Y E +++ + + D+PE + + +
Sbjct: 259 WESMADDVLRKQQRVLNFPQLELKAKE-LEKYTLIEIETLLRQHEKSLSDYPEMPQPEKS 317
Query: 391 LTKSVDMTLQKKKVKHDKQELEIDKEQ 471
+ + V+ +L +++ ++ IDKE+
Sbjct: 318 ILEEVNNSLLRQEF-----QINIDKER 339
>At4g23800.1 68417.m03422 high mobility group (HMG1/2) family
protein similar to HMG2B [Homo sapiens] GI:32335;
contains Pfam profile PF00505: HMG (high mobility group)
box
Length = 456
Score = 29.9 bits (64), Expect = 4.6
Identities = 28/114 (24%), Positives = 61/114 (53%), Gaps = 10/114 (8%)
Frame = +1
Query: 142 MTRFARAKGSKASNEKAPEDGTPWEVMKE--QLLKAKQFTDDAKKREEAEKQRIENYENF 315
M + R K +A ++K E+ +E Q+LK K+ TD+ K+E+A K++ EN
Sbjct: 317 MEEYKRTKEEEALSQKKEEEELLKLHKQEALQMLKKKEKTDNLIKKEKATKKK--KNEN- 373
Query: 316 LXEHKIQKXKATWCDFPESEKSQ--------NNLTKSVDMTLQKKKVKHDKQEL 453
+ +K +K +++ F + E+ + NN T + ++L+ K++ +++++
Sbjct: 374 VDPNKPKKPASSYFLFSKDERKKLTEERPGTNNATVTALISLKWKELSEEEKQV 427
>At1g56210.1 68414.m06460 copper chaperone (CCH)-related low
similarity to copper homeostasis factor
[GI:3168840][PMID:9701579] and farnesylated proteins
ATFP3 [GI:4097547] and GMFP7 [Glycine max][GI:4097573];
contains PF00403 Heavy-metal-associated domain
Length = 364
Score = 29.9 bits (64), Expect = 4.6
Identities = 20/68 (29%), Positives = 33/68 (48%)
Frame = +1
Query: 265 KKREEAEKQRIENYENFLXEHKIQKXKATWCDFPESEKSQNNLTKSVDMTLQKKKVKHDK 444
KK+++ EK +I N +K + CD PESEK + S D VK +K
Sbjct: 125 KKKKKEEKVQITNEATSSGIDNPEKPGSGECDKPESEKPVDEKCLSGDGGETSGPVKEEK 184
Query: 445 QELEIDKE 468
+++ +K+
Sbjct: 185 KDVLKEKD 192
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 29.9 bits (64), Expect = 4.6
Identities = 16/80 (20%), Positives = 37/80 (46%)
Frame = +1
Query: 238 KAKQFTDDAKKREEAEKQRIENYENFLXEHKIQKXKATWCDFPESEKSQNNLTKSVDMTL 417
K+ DD ++ +E +K+R+ E EH+ + K D E+ + D
Sbjct: 97 KSSDSEDDVEEEDERDKRRVNEKERGHREHERDRGKDRKRDREREERKDKEREREKDRER 156
Query: 418 QKKKVKHDKQELEIDKEQIE 477
++++ + ++E ++E+ E
Sbjct: 157 REREREEREKERVKERERRE 176
>At3g13225.1 68416.m01660 WW domain-containing protein contains Pfam
profile PF00397: WW domain
Length = 863
Score = 29.1 bits (62), Expect = 8.0
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = +1
Query: 124 SKVSVRMTRFARAKGSKASNEKAPEDGTPWEVMKEQLLKAKQFTDDAKKREEAEKQRIEN 303
+KV + A + SN+K W+ KE+L +++ DD++ + K+ IE
Sbjct: 714 TKVKRTKKKTVAATSTLRSNKKVSSLVDKWKAAKEELNDSEEEEDDSEILDRKRKREIEE 773
Query: 304 YEN 312
+++
Sbjct: 774 WKS 776
>At2g24440.1 68415.m02921 expressed protein
Length = 183
Score = 29.1 bits (62), Expect = 8.0
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +1
Query: 373 EKSQNNLTKSVDMTLQKKKVKHDKQELEIDKEQIE 477
EK + TK+ ++ KKKVK ++ ++I+KE+ E
Sbjct: 48 EKKKRKTTKAKNVGAAKKKVKKEEVAVKIEKEEEE 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,679,994
Number of Sequences: 28952
Number of extensions: 267372
Number of successful extensions: 731
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 694
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 724
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4048208640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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