BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030623sawa_G06_e47_14.seq (1568 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 50 7e-07 SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 44 4e-05 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 44 6e-05 SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 44 8e-05 SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 38 0.003 SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 38 0.003 SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 37 0.009 SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 36 0.015 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 35 0.035 SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 35 0.035 SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 34 0.047 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 34 0.062 SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 33 0.081 SPAPB17E12.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 33 0.11 SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 33 0.11 SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schi... 33 0.11 SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos... 32 0.25 SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharom... 32 0.25 SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 31 0.43 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 31 0.43 SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 31 0.57 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 30 1.0 SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 29 1.3 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 1.8 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 28 4.0 SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 27 5.3 SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 5.3 SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 27 5.3 SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 27 5.3 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 50.4 bits (115), Expect = 7e-07 Identities = 21/49 (42%), Positives = 30/49 (61%) Frame = +3 Query: 189 QGDVDEELICPICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPVDR 335 Q +V EL C IC G+L DP+ +P C H FC C+ + +++ P CP R Sbjct: 160 QENVSRELECQICFGMLYDPVVSP-CGHTFCGPCLMQALTQSPQCPTCR 207 >SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 963 Score = 44.4 bits (100), Expect = 4e-05 Identities = 19/51 (37%), Positives = 31/51 (60%), Gaps = 2/51 (3%) Frame = +3 Query: 162 DMGFEIKRFQGDVD--EELICPICSGVLEDPLQAPACEHAFCRACITEWIS 308 D+ KR D++ E ++C IC V +D +++ C H FCR C+TE+I+ Sbjct: 691 DLVLASKRKTVDIENQENIVCKICDEVAQDAIES-RCHHTFCRLCVTEYIN 740 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 44.0 bits (99), Expect = 6e-05 Identities = 18/54 (33%), Positives = 28/54 (51%) Frame = +3 Query: 177 IKRFQGDVDEELICPICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPVDRQ 338 ++ FQ + + L CPIC+ L+ P C H +C C+ W+ +CP RQ Sbjct: 73 LETFQ-KIKKTLECPICTEALQRPFTTH-CGHTYCYECLLNWLKESKSCPTCRQ 124 >SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 43.6 bits (98), Expect = 8e-05 Identities = 20/55 (36%), Positives = 26/55 (47%) Frame = +3 Query: 198 VDEELICPICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPVDRQAVTASQLR 362 +D L C IC PL +C H FC CI +++ P CP R S+LR Sbjct: 23 LDSSLRCLICHEYFRAPL-ITSCSHTFCSFCIRDYLREHPMCPACRAPEQESRLR 76 >SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 306 Score = 38.3 bits (85), Expect = 0.003 Identities = 16/48 (33%), Positives = 25/48 (52%) Frame = +3 Query: 216 CPICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPVDRQAVTASQL 359 C +C + P A C H FC +CI W S++ CP+ R + S++ Sbjct: 256 CSLCMEFIHCPA-ATECGHIFCWSCINGWTSKKSECPLCRAFSSPSKI 302 >SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 677 Score = 38.3 bits (85), Expect = 0.003 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +3 Query: 219 PICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPVDRQAVTASQLRP 365 P+ G+ P + P C H C+ W+ RQ TCP+ R++V +Q P Sbjct: 314 PLPRGLDMTPKRLP-CGHILHFHCLRNWLERQQTCPICRRSVIGNQSSP 361 >SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 741 Score = 36.7 bits (81), Expect = 0.009 Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 4/73 (5%) Frame = +3 Query: 138 IQSSTKKADMGFEIKRFQGDVDEELICPICSGVLE--DPLQAPA--CEHAFCRACITEWI 305 ++ + K+A FE D +L CPIC + D QA C H F + C+ +W+ Sbjct: 83 VKRAVKEAWDSFE--PLSNDQLMDLTCPICYDDMNENDEKQATKMPCGHIFGKNCLQKWL 140 Query: 306 SRQPTCPVDRQAV 344 TCP+ R+ V Sbjct: 141 ENHCTCPLCRKEV 153 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 35.9 bits (79), Expect = 0.015 Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 3/52 (5%) Frame = +3 Query: 198 VDEELICPICSGVL---EDPLQAPACEHAFCRACITEWISRQPTCPVDRQAV 344 +DEE C IC + +D +Q P C+H F CI W+ TC + R V Sbjct: 390 IDEEGECTICMEMFKINDDVIQLP-CKHYFHENCIKPWLRVNGTCAICRAPV 440 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 34.7 bits (76), Expect = 0.035 Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 1/75 (1%) Frame = +3 Query: 144 SSTKKADMGFEIKRFQGDVDEELICPICSGVLEDPLQAPACEHAFCRACITEWI-SRQPT 320 +S K + + F G + L+C + ++ + C H F R CI +W+ S Q + Sbjct: 504 ASQKDLEKAGGVFPFSGTDERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNS 563 Query: 321 CPVDRQAVTASQLRP 365 CP+ R AS P Sbjct: 564 CPLCRTKGVASASTP 578 >SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 34.7 bits (76), Expect = 0.035 Identities = 20/68 (29%), Positives = 29/68 (42%), Gaps = 1/68 (1%) Frame = +3 Query: 123 KETDVIQSSTKK-ADMGFEIKRFQGDVDEELICPICSGVLEDPLQAPACEHAFCRACITE 299 +E D +Q KK A + + + + D +C IC P+ A C H FC C Sbjct: 222 REWDSVQEKYKKGAKLEEGMVKNEKKEDIPFVCLICKKDYRSPI-ATTCGHHFCEQCAIT 280 Query: 300 WISRQPTC 323 + PTC Sbjct: 281 RYRKTPTC 288 >SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 34.3 bits (75), Expect = 0.047 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 3/46 (6%) Frame = +3 Query: 201 DEELICPICSG--VLEDPLQAPACEHAFCRACITEWI-SRQPTCPV 329 DEE C IC +D L+ CEH F CI W+ + + +CP+ Sbjct: 200 DEEDFCIICYADYAFDDILRVLPCEHVFHTQCIDTWMTTMKASCPL 245 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 33.9 bits (74), Expect = 0.062 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +3 Query: 213 ICPICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPV 329 IC IC +++ C H +C C+ W+ +CP+ Sbjct: 1091 ICIICRDIIKQGF-ITTCGHLYCSFCLEAWLKHSSSCPM 1128 >SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 33.5 bits (73), Expect = 0.081 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = +3 Query: 132 DVIQSSTKKADMGFEIKRFQGDVDEELICPICSGVLEDPLQAPACEHAFCRACITEWI 305 D + S +KA +G + + V E C IC P + C+H FC AC +++ Sbjct: 103 DAPEESLQKAGVGLSGSKQREVVHHEGTCEICYDEGCLPFFSAECDHEFCLACYRQYL 160 >SPAPB17E12.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 311 Score = 33.1 bits (72), Expect = 0.11 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 1/49 (2%) Frame = +3 Query: 186 FQGDVDEELICPICSGVLEDPLQAPACEHAFCRACITEWISRQP-TCPV 329 F+ +V IC IC +++P + FC CI W+ R P CPV Sbjct: 247 FEINVSTTDICKICGEKIKNPAVL-STGFVFCYPCIQVWLQRHPFKCPV 294 >SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 860 Score = 33.1 bits (72), Expect = 0.11 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 201 DEELICPICSGVLEDPLQAPACEHAFCRACITEWISRQPTC 323 D E +C C G+L+ P + +C H R C TE + P C Sbjct: 788 DSEKVCDNCEGLLDVPFVSYSCLHLVHRDCATETVC--PKC 826 >SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schizosaccharomyces pombe|chr 1|||Manual Length = 94 Score = 33.1 bits (72), Expect = 0.11 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 4/45 (8%) Frame = +3 Query: 216 CPICSGVLED-PLQAPACEHAFCRACITEWIS---RQPTCPVDRQ 338 CP C+ ++ P+ C+H F CI W++ Q CP+DRQ Sbjct: 35 CPQCTSPGDNCPIVWGKCKHIFHAHCIQNWLATSGSQGQCPMDRQ 79 >SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizosaccharomyces pombe|chr 2|||Manual Length = 482 Score = 31.9 bits (69), Expect = 0.25 Identities = 16/54 (29%), Positives = 23/54 (42%), Gaps = 2/54 (3%) Frame = +3 Query: 210 LICPICSGVLEDPLQAPACEHAFCRACI-TEWISRQPTCP-VDRQAVTASQLRP 365 L C +C + + + P C+ FC CI T + CP R+ V L P Sbjct: 280 LACTLCKKLARNACRTPCCDKLFCEECIQTALLDSDFECPNCHRKDVLLDTLNP 333 >SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 107 Score = 31.9 bits (69), Expect = 0.25 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +3 Query: 264 CEHAFCRACITEWISRQPTCPVDRQ 338 C HAF CI+ W++ + CP+D + Sbjct: 74 CNHAFHFHCISRWLNTRNVCPLDNR 98 >SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosaccharomyces pombe|chr 3|||Manual Length = 680 Score = 31.1 bits (67), Expect = 0.43 Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 1/52 (1%) Frame = +3 Query: 216 CPICSGVLEDPLQAPACEHAFCRACITEWI-SRQPTCPVDRQAVTASQLRPV 368 C +C+ C H FC CI + I +RQ CP+ + AS + P+ Sbjct: 627 CSVCNFERWKDRIISLCGHGFCYQCIQKRIETRQRRCPICGRGFGASDVIPI 678 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 31.1 bits (67), Expect = 0.43 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 3/49 (6%) Frame = +3 Query: 177 IKRFQGDVDEELICPICSGVLEDPLQAPA---CEHAFCRACITEWISRQ 314 +K F+ + E CPIC +P+Q P C+HA C C++E I Q Sbjct: 867 LKSFEALITE---CPICCN---EPIQNPLLLNCKHACCGDCLSEHIQYQ 909 >SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1040 Score = 30.7 bits (66), Expect = 0.57 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +3 Query: 204 EELICPICSGVLEDPLQAPACEHAFCRACITEWIS 308 E L C +C V+ + L C H CR C+T I+ Sbjct: 742 ETLQCSLCMDVVAELLIIVPCGHFLCRECLTHVIT 776 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 29.9 bits (64), Expect = 1.0 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = +3 Query: 264 CEHAFCRACITEWISRQPTCPVDR 335 C H + R C+ +W+ + CPV R Sbjct: 647 CHHLYHRQCLLQWMETRSICPVCR 670 >SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 29.5 bits (63), Expect = 1.3 Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 3/40 (7%) Frame = +3 Query: 216 CPIC--SGVLEDPLQAPACEHAFCRACITEWI-SRQPTCP 326 C IC S D + A C+H F R CI +WI + CP Sbjct: 321 CVICLESFTKGDKVVALPCKHEFHRPCIAKWIVDYRHACP 360 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 29.1 bits (62), Expect = 1.8 Identities = 11/26 (42%), Positives = 18/26 (69%), Gaps = 1/26 (3%) Frame = +3 Query: 261 ACEHAFCRACITEWISR-QPTCPVDR 335 AC+H F +ACI +W++ +CP+ R Sbjct: 784 ACKHFFHQACIDQWLTTGNNSCPLCR 809 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 27.9 bits (59), Expect = 4.0 Identities = 13/58 (22%), Positives = 27/58 (46%) Frame = +3 Query: 192 GDVDEELICPICSGVLEDPLQAPACEHAFCRACITEWISRQPTCPVDRQAVTASQLRP 365 GD+ + + P+ +++DP+ P + R+ I + T P +R +T + P Sbjct: 929 GDIPDYFLDPLMFTIMKDPVVLPRSGISIDRSTIKAHLLSDATDPFNRTPLTLDDVTP 986 >SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 Brl1|Schizosaccharomyces pombe|chr 3|||Manual Length = 692 Score = 27.5 bits (58), Expect = 5.3 Identities = 13/40 (32%), Positives = 18/40 (45%), Gaps = 1/40 (2%) Frame = +3 Query: 210 LICPICSGVLEDPLQAPACEHAFCRACITEWIS-RQPTCP 326 L C +C+ P C HAFC C+ + + TCP Sbjct: 637 LKCSVCNFSNWKSKLIPNCGHAFCSNCMEPFYEHKTSTCP 676 >SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 27.5 bits (58), Expect = 5.3 Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 3/46 (6%) Frame = +3 Query: 216 CPICSGVLEDPLQAPA--CEH-AFCRACITEWISRQPTCPVDRQAV 344 C IC + +D L+A C H F +CI W+ + TCP+ ++ V Sbjct: 21 CVIC--LQKDGLRAQLSPCGHDQFDYSCICRWMDQSLTCPICKRHV 64 >SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 205 Score = 27.5 bits (58), Expect = 5.3 Identities = 13/40 (32%), Positives = 18/40 (45%), Gaps = 2/40 (5%) Frame = +3 Query: 255 APACEHAFCRACITEWISRQPTCPVD--RQAVTASQLRPV 368 A C H FC C E + CPV R+ +T + P+ Sbjct: 164 AAKCGHLFCSTCAKELRKKTVPCPVQHCRKRITKKFIFPL 203 >SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 27.5 bits (58), Expect = 5.3 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = +3 Query: 450 LDSLANHLVECEFNPKRPMPCEAGCGLVIPK 542 +D+L +HL E F R + CE GLV P+ Sbjct: 116 IDALESHLSEASFPSLRSVLCERYPGLVSPE 146 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,481,176 Number of Sequences: 5004 Number of extensions: 79463 Number of successful extensions: 192 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 884049604 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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